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  • 1
    Electronic Resource
    Electronic Resource
    No evidence of mycoplasmas in peripheral blood mononuclear cell fraction of HIV-infected patients (1994)
    Springer
    Infection 22 (1994), S. 204-206 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei HIV-infizierten Patienten wurde mittels eines Genus-spezifischen PCR-Assay versucht, die Prävalenz von Mykoplasmen in mononukleären Zellen des peripheren Blutes zu bestimmen. In keinem der 25 untersuchten Fälle (CDC-Stadium 2, n=8; Stadium 3, n=2; Stadium 4, n=15) und zehn HIV-seronegativen Kontrollen waren Mykoplasmen in peripheren mononukleären Zellen nachzuweisen. In allen HIV-seropositiven Fällen waren HIV-spezifische Sequenzen mittels PCR nachzuweisen, was als interne Kontrolle dienen kann. Diese negativen Ergebnisse unterstützen die Hypothese nicht, daß Mykoplasmen eine Bedeutung als Kofaktoren der Progression der HIV-Infektion zu AIDS zukommt.
    Notes: Summary In this study, the prevalence of mycoplasmas in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals was investigated using a mycoplasma genusspecific PCR assay. No mycoplasmas were detected in the PBMC samples from any of the 25 HIV-infected individuals (CDC 2, n=8; CDC 3, n=2; CDC 4, n=15) or ten HIV-seronegative controls. As an internal control, HIV specific sequences were detected in the samples from all HIV-seropositives. These negative results do not support a suggested role of mycoplasmas as co-factor in the progression of HIV infection towards AIDS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01716704
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  • 2
    Electronic Resource
    Electronic Resource
    16S rRNA based polymerase chain reaction compared with culture and serological methods for diagnosis ofMycoplasma pneumoniae infection (1994)
    Springer
    European journal of clinical microbiology & infectious diseases 13 (1994), S. 401-405 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The use of a 16S rRNA based polymerase chain reaction (PCR) for the detection ofMycoplasma pneumoniae infection was investigated. Sputum samples from 34 patients with respiratory illness and evidence of pneumonia as judged by chest X-ray were analyzed by PCR and microbiological culture. Throat swabs from 14 healthy individuals were used as controls. For serology, an enzyme immunoassay for the detection of immunoglobulin M antibodies and a complement fixation assay were performed. Evidence ofMycoplasma pneumoniae infection was obtained in ten patients (29 %), eight of whom were found positive by both PCR and serology. Two of the sputum samples from these eight patients were negative by culture. Of the remaining two patients positive forMycoplasma pneumoniae, one was positive by PCR and culture but negative by serology, and one was found positive by serology but negative by PCR and culture. Thirteen of the 14 controls were negative by both PCR and serology. One control, however, was negative by serology but positive by PCR, which was probably due to asymptomatic carriage ofMycoplasma pneumoniae. The results of this study indicate the suitability of the PCR for the detection ofMycoplasma pneumoniae in clinical samples as well as its potential value as an additional tool for the diagnosis of infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01971997
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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