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  • 1
    Electronic Resource
    Electronic Resource
    Coamplification of hprt cDNA and γ T-cell receptor sequences from 6-thioguanine resitant human T-lymphocytes
    Amsterdam : Elsevier
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 288 (1993), S. 269-275 
    Details
    ISSN: 0027-5107
    Keywords: Hprt ; Human T-lymphocytes ; Reverse transcription ; γ T-cell receptor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0027-5107(93)90094-V
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  • 2
    Electronic Resource
    Electronic Resource
    Radiation-induced base substitution mutagenesis in single-stranded DNA phage M13 (1981)
    [s.l.] : Nature Publishing Group
    Nature 294 (1981), S. 180-182 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We used single-stranded DNA phage Ml 3 to study independently the effects of target DNA irradiation and the consequences of induction of the SOS repair system. M13 is particularly useful in such an investigation because (1) mutagenesis of single-stranded DNA phages by UV and y irradiation is ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/294180a0
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of thymidylate biosynthesis induces mitotic unequal sister chromatid recombination in Saccharomyces cerevisiae (1984)
    Springer
    Current genetics 8 (1984), S. 211-217 
    Details
    ISSN: 1432-0983
    Keywords: dTMP starvation ; Sister chromatid recombination ; rad mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inhibition of thymidylate biosynthesis has been found to induce deletion of a LEU2 insert from the ribosomal DNA gene cluster of haploid strains of Saccharomyces cerevisiae. Loss of the insert was detected phenotypically by the enhanced production of both sectored (leu+/leu−) and non-sectored (leu−) colonies. Hybridization patterns obtained by Southern blot analysis of DNA from the leu+ and leu− sectors were consistent with the occurrence of unequal sister chromatid recombination. The induction of sectored colonies was prevented by the rad52-1 mutation but not by defects in RAD6. However, the formation of non-sectored leu− colonies was induced by thymidylate depletion in both rad52-1 and rad6 strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417818
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  • 4
    Electronic Resource
    Electronic Resource
    Perspectives on UV light mutagenesis: Investigation of the CHOaprt gene carried on a retroviral shuttle vector (1989)
    Springer
    Somatic cell and molecular genetics 15 (1989), S. 401-409 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The extent to which the cellular processing of shuttle vector-carried genes reflects that of endogenous chromosomal loci has been a subject of considerable controversy. In order to address this issue, we have developed a retroviral-based shuttle vector carrying the Chinese hamster ovary (CHO) adenine phosphoribosyltransferase (aprt) gene stably integrated into the genome to be used for studying mutational specificity in mammalian cells. Initially, we have characterized a collection of UV-induced mutants in a CHO cell background. We have therefore been able to directly compare this shuttle vector data to that previously obtained for UV-induced mutation at the endogenous CHO (aprt)locus. Although some potential differences between the two spectra have been noted, there appears to be a remarkable similarity in the distribution and site specificity of UV-induced mutations. These similarities extend to extrachromosomal shuttle vectors as well and consolidate the role of shuttle vectors as powerful analytical tools for studying mechanisms of point mutagenesis in mammalian cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01534891
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  • 5
    Electronic Resource
    Electronic Resource
    Sequence specificity of mutations induced by benzo[a]pyrene-7,8-diol-9,10-epoxide at endogenousaprt gene in CHO cells (1988)
    Springer
    Somatic cell and molecular genetics 14 (1988), S. 393-400 
    Details
    ISSN: 1572-9931
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have determined the spectrum of mutations induced, by±trans-7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a] pyrene (BPDE) at the endogenous aprt locus in an hemizigous Chinese hamster ovary cell line exposed to 0.7 μM BPDE. Southern analysis of 59 independent mutants revealed no major genomic alterations, indicating that gene inactivation was the result of a point mutation. This conclusion was confirmed by the cloning and sequencing of 21 of these mutants. The predominant mutation, the G∶CT→T∶A transversion, comprised 62% of the spectrum, but other base pair substitutions and frameshifts were recovered. An examination of the target sequences for BPDE mutation revealed that mutations were localized within runs of G∶C base pairs. However, approximately half of these G∶C runs involved a particular sequence—a run of guanines flanked by adenine residues. Of seven such sites within the coding sequence ofaprt, mutations were clustered within five of them. This class of sequence occurs at codon 61 of the human C-Ha-ras1 protooncogene and may account for the selective activation of this codon by BPDE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01534647
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  • 6
    Electronic Resource
    Electronic Resource
    A mutant of Escherichia coli K12 deficient in the 5′–3′ exonucleolytic activity of DNA polymerase I (1973)
    Springer
    Molecular genetics and genomics 124 (1973), S. 69-82 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new mutation affecting DNA polymerase I of Escherichia coli is described. Strains carrying mutation polA107 are similar to polA1 strains in their sensitivity to methyl methanesulphonate (MMS), thymine deprivation, their reduced ability to repair MMS treated phage λ and are unable to propagate a phage λred - mutant. Like the polA1-recBC combination, polA107-recBC double mutants are inviable. However, in contrast to polA1 mutants, polA107 mutants grow almost normally in the presence of acridine orange. PolA107 bacteria are more sensitive to UV and X-ray irradiation than Pol+ strains but not as sensitive as polA1 strains. Following X-ray irradiation, DNA degradation in the polA107 strains is as extensive as in the polA1 strain. X-ray induced single-strand breaks, however, are repaired in the polA107 strain but not in the polA1 strain. Following UV irradiations in contrast to the polA1 strain, only low levels of DNA degradation were observed in the polA107 strain. Complementation for MMS or radiation resistance between the polA107 and polA1 mutations was not observed. In the following paper it is shown that the polA107 strain contains a normal level of DNA polymerizing activity but lacks the associated 5′–3′ exonucleolytic activity found in DNA polymerase I.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267166
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  • 7
    Electronic Resource
    Electronic Resource
    A mutant of Escherichia coli K12 deficient in the 5′–3′ exonucleolytic activity of DNA polymerase I (1973)
    Springer
    Molecular genetics and genomics 124 (1973), S. 83-96 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The enzymatic properties of purified DNA polymerase I from a strain of Escherichia coli K12 with a mutation in the polA gene have been studied. The polymerizing activity of the mutant enzyme is similar to that of the enzyme from isogenic wild-type cells, when the activity is measured on exonuclease III treated calf-thymus DNA. Also the 3′–5′ exonucleolytic activity is not significantly different for both enzyme preparations. The 5′–3′ exonucleolytic activity of DNA polymerase I isolated from the mutant strain, however, is much lower than that of wild-type DNA polymerase I. The products formed by the action of the wild-type and the mutant enzyme on nicked circular double-stranded DNA of phage ϕX174′ (RFII DNA) were analysed by sucrose-gradient sedimentation and electron-microscopy. When RFII DNA was incubated with wild-type enzyme 80% of the molecules were converted into linear molecules. All linear molecules were shorter than one phage genome. Only 25% of the molecules were branched. After incubation of RFII DNA with the mutant enzyme 62% of the molecules have become linear. More than 90% of these linear molecules were branched and the majority of them was longer than one phage genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267167
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  • 8
    Electronic Resource
    Electronic Resource
    The genetic characterization of lexB32, lexB33 and lexB35 mutations of Escherichia coli: Location and complementation pattern for UV resistance (1977)
    Springer
    Molecular genetics and genomics 157 (1977), S. 83-89 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of LexB have been isolated by their resistance to lysogenic induction by thymine starvation, their resistance to thymine starvation and on the basis of their UV sensitivity. Here, three mutations identified originally in strains lacking mutagenic response to UV-irradiation, unmB (Kato and Shinoura, 1977), have been further characterized, mapped by P1-mediated transduction with srl into the recA-tif-zab-lexB cluster at the lexB position and analysed for complementation with various lexB and recA mutations. From the results it was concluded that unmB mutations are identical to lexB mutations; consequently these mutations have been termed lexB32, lexB33 and lexB35. The mutations lexB33 and lexB35 do not complement any of the other lexB mutations and define therefore a new complementation type. The lexB32 mutation, which like the lexB34 mutation, results in moderate UV sensitivity has a complementation pattern similar to that of lexB34. However, unlike lexB34 the lexB32 behaves like a leaky mutation. The results are discussed in relation to the recA gene product and its control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268690
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