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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 15 (1985), S. 0 
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The prevalence of storage mite allergy was investigated in an epidemiological study of respiratory symptoms in farmers on Gotland, an island in the Baltic Sea. A questionnaire concerning work-related and chronic symptoms from the airways and eyes was completed by 2578 farmers. A sample of 440 farmers subsequently underwent examination comprising skin-prick tests (animal danders, pollens, moulds and house dust mite), blood sampling for RAST against four storage mites (Acarus siro, Lepidoglyphus destructor. Tyrophagus putrescentiae and Glycyphagus domesticus) and total IgE analyses. Immediate onset hypersensitivity reactions in the airways and eyes were very common among active farmers on Gotland, the prevalence being as high as 40.0%. The prevalence of atopic allergy was 15.6%. Allergy to storage mites was diagnosed, from the case history and a positive RAST to at least one of the four storage mites, in fifty-two of 440 studied farmers (12%), corresponding to a calculated prevalence of storage mite allergy in the whole farming population of Gotland of 6.2%. The corresponding prevalence among farmers with hypersensitivity symptoms was 15.4%and among those with possibly IgE-mediated symptoms it was as high as 37.8%. The present study clearly demonstrates a high prevalence of allergy to storage mites among farmers with respiratory symptoms.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Initially, parainfluenza-3 (PI-3) virus was chosen since we had previously used its envelope glycoproteins in experiments with a micelle vaccine3,12. The glycoproteins were prepared by centrifugation of the solubilized virus layered on a sucrose gradient containing 0.2% Quil A. The details of this ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0851
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Of 112 patients (maximum age 70 years) with acute nonlymphocytic leukemia, 62 (55%) went into remission on an induction therapy of cytosine arabinoside and daunorubicin. 20 patients were randomized for maintenance treatment consisting of chemotherapy only and 22 patients for combined chemo-immunotherapy. The chemotherapy consisted in 5-day courses of daunorubicin and cytosine arabinoside and of thioguanine and cytosine arabinoside, alternating every month. The chemo-immunotherapy group also received weekly intracutaneous injections of 109 allogeneic nonirradiated leukemic myeloblasts and 106 BCG organisms (Glaxo) by Heaf gun. The median duration of the first remission was 164 days for the chemotherapy group and 464 days for the chemo-immunotherapy group. The corresponding median times of survival were 344 days for the first group and 734 days for the second group. The difference concerning median duration of survival is statistically significant. Thus immunotherapy seems to prolong survival.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Archives of virology 53 (1977), S. 323-333 
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Parainfluenza-3 virus was isolated by affinity chromatography, including a purification step with immobilized lectinVicia erwilia. The peplomers of disintegrated virus were similarily isolated using another carbohydrate-specific lectinVicia crotalaria. The whole virion and the peoplomers were both active as antigens in the leucocyte migration inhibition, lymphocyte stimulation and skin hypersensitivity tests. The remaining virus material, freed of detergents used for virus disintegration and containing nucleocapsids, did not act as antigen in these tests of cell-mediated immunity.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Planta 169 (1986), S. 33-37 
    ISSN: 1432-2048
    Keywords: Carthamus ; Microsome ; Oil-body formation ; Triacylglycerol synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microsomal membrane preparations from the developing cotyledons of safflower (Carthamus tinctorius L.) seed catalyse the formation of triacylglycerol fromsn-glycerol 3-phosphate and linoleoyl-CoA. Conditions of incubation were achieved in which the rate of triacylglycerol synthesis approached activities which were compatible with oil accumulation observed in vivo. Reaction mixtures which contained the microsomes took on a white soup-like appearance as triacylglycerol synthesis proceeded and sufficient oil was produced to form a white fat-pad at the surface after centrifugation. The development of the oil bodies in the microsomal membranes was studied by electron microscopy and showed that lipid droplets were formed in or on the membrane surface and were then released as apparently naked entities into the surrounding medium. The ontogeny of the oil droplet in vitro is discussed in terms of oil-body formation in vivo.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1573-2576
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The inflammatory response, antigen retention, and antigen localization was studied in mice after immunization with influenza virus glycoproteins presented in two physically defined forms-micelles and ISCOMS (immunostimulating complexes). Two hours after intraperitoneal injection, the proportion of polymorphonuclear leukocytes (PMNs) in peritoneal lavage cells increased from less than 1% to 82% in ISCOM-treated mice and from 〈1% to 41% of the total cell count in micelle-treated mice. For both treatment groups, the proportion of PMNs returned to around zero 24 h postimmunization. Total recovery of radioactive antigen was significantly greater (P〈0.05) in ISCOM-treated than in micelle-treated mice at one, two, and eight days postinjection. At all times tested, animals given ISCOMs had significantly more radioactive antigen in their spleens than animals given micelles. By electron microscopy ISCOMs were found to attach externally to the plasma membrane or within phagosomes of macrophages in close association with the membranes.
    Type of Medium: Electronic Resource
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