Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Light and electron microscopic localization of GABAA-receptors on cultured cerebellar granule cells and astrocytes using immunohistochemical techniques (1991)
    Springer
    Neurochemical research 16 (1991), S. 341-346 
    Details
    ISSN: 1573-6903
    Keywords: GABAA-receptors ; neurons ; astrocytes ; immunogold labeling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract GABAA-receptors were localized in explant cultures of rat cerebellum and in dissociated primary cultures of rat cerebellar granule cells and rat cerebellar astrocytes using the monoclonal antibody bd-17 directed against the β-subunit of the GABAA/benzodiazepine/chloride channel complex. At the light microscope level specific staining of GABAA-receptors was localized in various types of neurones in explant cultures of rat cerebellum using the indirect peroxidase-antiperoxidase (PAP) technique, whereas no specific staining was found in astrocytes. At the electron microscope level labeling of GABAA-receptors was observed in the plasma membrane of both the cell bodies and processes in dissociated primary cultures of cerebellar granule cells using an indirect preembedding immunogold staining technique which in contrast to the classical PAP technique allows quantitative estimations to be performed. Quantification of the labeling intensity revealed a higher concentration of GABAA-receptors per μm plasma membrane in the cell bodies than in the processes. In discrete areas an extremely high density of the GABAA-receptors was observed. No specific labeling of GABAA-receptors was observed in dissociated primary cultures of cerebellar astrocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00966097
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Uptake of3H-noradrenaline and3H-5-hydroxytryptamine in cultured rat brainstem (1975)
    Springer
    Cellular and molecular life sciences 31 (1975), S. 354-356 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Die zelluläre Lokalisation der Aufnahme von3H-NA und3H-5HT in den Hirnstamm von Ratten wurde am Modell der Nervengewebskultur untersucht. Im Gegensatz zu den Aminosäure-Transmittern, welche in Neurone und Gliazellen aufgenommen werden, zeigen Monoamine nur eine Aufnahme in Zellkörper und Fortsätze von Neuronen, nicht jedoch in Gliazellen. Es besteht eine Korrelation zwischen den Ergebnissen von fluoreszenzmikroskopischen Versuchen und unseren autoradiographischen Untersuchungen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01922583
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Action of GABA and glycine on the membrane potential of cultured astrocytes and the extracellular K+-concentration (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 1396-1396 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The depolarization of cultured astrocytes by GABA and glycine correlates in amplitude and time course with the increase of the extracellular K+-concentration during perfusion with these amino acids. It is suggested that the glial depolarization is caused by an efflux of K+ from neighbouring neurones activated by the amino acid transmitters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01960118
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Uptake ofl-glutamate andl-aspartate in neurones and glial cells of cultured human and rat spinal cord (1976)
    Springer
    Cellular and molecular life sciences 32 (1976), S. 219-222 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiographic investigations on the uptake ofl-glutamate andl-aspartate have shown that the amino acids were taken up by neurones as well as by glial cells of cultured human and rat spinal cord. The activity of glutamate and aspartate varied considerably between individual neurones, whereas glial cells showed a more even distribution of the labelled amino acids. Our results suggest that both neurones and glial cells are involved in the uptake of amino acid transmitters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01937776
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Ionic mechanisms underlying the depolarization of L-glutamate on rat and human spinal neurones in tissue culture (1973)
    Springer
    Cellular and molecular life sciences 29 (1973), S. 1244-1247 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Die Wirkung von Glutamat wurde auf das Membranpotential von Rückenmarkneuronen des Menschen und der Ratte in Gewebekultur untersucht. Entfernung der Natriumionen aus der extrazellulären Flüssigkeit führt zu einem Verschwinden der durch Glutamat erzeugten Depolarisation. Diese Befunde weisen darauf hin, dass Glutamat, welches eine vermutliche Überträgersubstanz im Rückenmarkist, die Permeabilität der Neuronenmembran für Natriumionen erhöht. Die Versuche zeigen ferner, dass die Gewebekultur ein ausgezeichnetes Modell ist zur Abklärung von ionalen Mechanismen, welche der Wirkung von Überträgersubstanzen im Zentralnervensystem zugrunde liegen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01935098
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    The effects of methyl mercury on morphological and histochemical properties of human and rat spinal cord and cerebellum in tissue culture (1974)
    Springer
    Cellular and molecular life sciences 30 (1974), S. 1300-1304 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung An Nervengewebskulturen menschlicher Foeten und neugeborener Ratten wurden Untersuchungen über die toxische Wirkung von Methylquecksilber (MMC) auf Neurone und Gliazellen im Rückenmark und Cerebellum durchgeführt. Die durch MMC erzeugte Degeneration zeigte eine deutliche Abhängigkeit von der Konzentration (10−6–10−4 M) sowie von der Expositionszeit. Die histochemischen Befunde zeigen, dass MMC den Gehalt von Succinatdehydrogenase stark vermindert, die Acetylcholinesterase-Aktivität jedoch kaum beeinflusst.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01945197
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Autoradiographic localization of the uptake of3H-β-alanine in rat nervous tissue cultures (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 1519-1521 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Autoradiographic studies on the uptake of3H-β-alanine have shown that, in spinal cord and brain stem cultures, both neurones and glial cells have accumulated the amino acid. In contrast, in cultures of cerebellum and dorsal root ganglia,3H-β-alanine was only taken up by glial elements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01932387
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Ionic mechanisms associated with the depolarization by glutamate and aspartate on human and rat spinal neurones in tissue culture (1976)
    Springer
    Pflügers Archiv 363 (1976), S. 43-48 
    Details
    ISSN: 1432-2013
    Keywords: Ionic mechanisms ; Amino acid depolarization ; CNS tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The action of glutamate and aspartate was studied on the membrane potential of human and rat spinal neurones in tissue culture. Both amino acids caused a depolarization of the cell membrane, the size of which was dependent on the concentration of the amino acids in the bathing fluid. In order to study ionic mechanisms associated with the amino acid depolarization, the ionic composition of the extracellular fluid was altered. Removal of sodium ions from the bathing solution reversibly reduced or abolished the depolarization produced by glutamate and aspartate suggesting that the action of these amino acids is associated with an increased sodium permeability. Substituting lithium for sodium ions also reversibly abolished the depolarization by glutamate indicating that in contrast to the effect of lithium on the action potential, this ion cannot replace sodium for the glutamate depolarization. These experiments show that the method of tissue culture is a suitable model to study ionic mechanisms underlying the action of neurotransmitters in the mammalian and especially in the human CNS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00587400
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...