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  • 1
    Electronic Resource
    Electronic Resource
    Immunocytochemical staining of Histoplasma capsulatum at the electron microscopic level (1986)
    Springer
    Mycopathologia 94 (1986), S. 157-161 
    Details
    ISSN: 1573-0832
    Keywords: H. capsulatum ; immunocytochemistry ; electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rabbits were immunized with histoplasmin emulsified in Freund's complete adjuvant. Antibody raised in these rabbits was exposed to Histoplasma capsulatum yeast cells, either in tissue culture medium, or after in vitro or in vivo phagocytosis by mouse macrophages. The sites of antibody binding were identified using an immunoperoxidase technique. At least two sites of antibody binding were identified, one to the fungal cell wall and the other to the outer cell membrane. Within 6 h after phagocytosis by macrophages, fungal cell walls appeared roughened, with what appeared to be cell wall antigen released into the phagolysosome, appearing associated with the phagolysosome membrane, and possibly adjacent macrophage cytoplasm. Similar staining of fungal antigen was noted in alveolar macrophages which had ingested Histoplasma capsulatum after a respiratory challenge. This method may be useful in detailing the host/pathogen interactions which occur in human pulmonary histoplasmosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454594
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  • 2
    Electronic Resource
    Electronic Resource
    Cytochemical evidence for the existence of norepinephrine-containing glomus cells in the rat carotid body (1983)
    Springer
    Journal of neurocytology 12 (1983), S. 1041-1053 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Some investigators have postulated that glomus cells of the rat carotid body contain only dopamine (DA), and that the norepinephrine (NE) measured in the carotid body resides only in sympathetic nerve endings and ganglion cells. To investigate this hypothesis, we employed a pharmacologic drug sequence which depleted all carotid body catecholamines and then selectively restored DA levels while keeping NE levels significantly lowered. Analysis of carotid body catecholamines by high performance liquid chromatography (HPLC) validated this drug regimen. Employing this drug treatment, we examined glomus cells after potassium dichromate cytochemical staining in an effort to distinguish those glomus cell vesicles which still contained appreciable amounts of catecholamine, presumably DA. Glomus cells from rats receiving vehicle orl-dopa (100 mg kg−1 ip) alone had 83 and 97% of their cells stained, respectively. However, glomus cells from reserpinized (5 mg kg−1 ip) animals were largely unstained (89%). Carotid bodies from animals treated with reserpine and then, 24 h later, withl-dopa 90 min prior to sacrifice had about 46% of their glomus cells stained while 54% of the cells were unstained. The results of this last group, coupled with our biochemical data which demonstrated that DA levels were comparable to control values but that NE was 80% depleted, suggest that a significant number of glomus cells did not contain enough catecholamine to react with the dichromate. We believe that these unstained cells may normally contain NE and that glomus cells may be of several types, some containing predominantly DA and others NE.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01153349
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of the primate carotid body: a morphometric study of the glomus cells and nerve endings in the monkey (Macaca fascicularis) (1985)
    Springer
    Journal of neurocytology 14 (1985), S. 13-32 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The carotid body of the monkey (Macaca fascicularis) was studied at both the light and electron microscopic levels in an effort to provide a detailed quantitative characterization of this chemoreceptor organ in the primate. Structurally, the monkey carotid body was organized into lobules of from three to eight glomus cells (in section) and their ensheathing supporting cells. Interspersed among the lobules was abundant connective tissue stroma, fibroblasts and mast cells. Fenestrated capillaries, small arterioles and venules also permeated the organ. Each supporting cell partially ensheathed about three glomus cells and could be easily differentiated from glomus cells by their darker cytoplasmic staining, lack of dense-core vesicles and angular nuclear profile. Glomus cells exhibited an intense catecholamine histofluorescence and contained abundant dense-core vesicles. On the basis of dense-core vesicle size, shape and numerical density, four types of glomus cells were identified. The most common type (62% of all glomus cells) contained vesicles with an average diameter of 219 nm and a density of 8 vesicles per μm2 of cytoplasm. The second type possessed larger vesicles (264 nm in diameter) and accounted for about 14% of all glomus cells. A third type of glomus cell contained smaller (167 nm) and fewer (5 vesicles per μm2) dense-core vesicles. The fourth type of glomus cell contained pleomorphic-shaped vesicles with a maximal diameter of 232 nm. Each of these last two types accounted for about 12% of all glomus cells. All four types of glomus cells were innervated, averaging 1.43 nerve endings per glomus cell (in sections). Nerve endings were primarily of the bouton-like variety averaging 2 μm2 in sectional area and containing 34.3 clear-core synaptic vesicles (average size 73.5 nm in diameter) per μm2 of cytoplasm. Of the 57 nerve endings examined in single sections, 16% displayed junctions typical of synaptic specializations and most of these were presynaptic to glomus cells. Glomus cell-glomus cell synapses were not observed. Based on these quantitative observations and on previous studies of carotid body cytoarchitecture in other laboratory species, it appears that the primate organ most closely resembles the cat carotid body, although several differences exist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01150260
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  • 4
    Electronic Resource
    Electronic Resource
    Dopamine β-hydroxylase-like immunoreactivity in the rat and cat carotid body: a light and electron microscopic study (1985)
    Springer
    Journal of neurocytology 14 (1985), S. 131-144 
    Details
    ISSN: 1573-7381
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Immunocytochemical localization of dopamine β-hydroxylase (DBH) was used to study the synthesis and storage sites of norepinephrine (noradrenaline) in the rat and cat carotid bodies. In the rat carotid body some parenchymal cells exhibited strong DBH-like immunoreactivity (DBH-I), while others displayed only faint DBH-I. In a typical parenchymal cell cluster, most cells with strong DBH-I were irregular in shape and appeared to partially surround those with weak DBH-I which usually were rounded in contour. In the cat carotid body most parenchymal cells showed a strong to moderate DBH-I. In both the rat and cat carotid bodies varicose nerve fibres with DBH-I were associated primarily with blood vessels. All autonomic ganglion cells examined, which were associated with the rat carotid body, showed DBH-I. Electron microscopy revealed that most DBH-I in the strongly positive cells of the rat carotid body was associated with dense granules (possibly corresponding to dense-cored vesicles of various sizes), although some was found in other sites. In oval cells with less DBH-I, reactivity resided in some of the large granules. In the cat carotid body the glomus cells contained more granules of various sizes and shapes than did those of the rat carotid body. Most of the cat glomus cell granules exhibited DBH-I activity. Our results indicate that some of glomus cells in the rat and most of the glomus cells in the cat contain DBH and therefore may be sites of norepinephrine synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01150267
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  • 5
    Electronic Resource
    Electronic Resource
    The use of small group case-based exercises in human gross anatomy: A method for introducing active learning in a traditional course format (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Clinical Anatomy 7 (1994), S. 357-366 
    Details
    ISSN: 0897-3806
    Keywords: small groups ; case studies ; gross anatomy ; active learning ; Life and Medical Sciences ; Miscellaneous Medical
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: As part of the curricular change at the University of Rochester School of Medicine and Dentistry, we focused on active learning and greater integration of basic and clinical sciences. With these objectives in mind, this report describes the use of small-group, case-based exercises in our gross anatomy course and provides one example of integrating such activities into a traditional course. In addition to formal lectures and laboratory dissection, students meet approximately every fourth class period in small groups to discuss a clinical case which focuses on the relevant anatomy taught at that time. Two first-year students lead each small group; one fourth-year student facilitator also attends to provide clinical correlations, answer questions, and reinforce the anatomy. Formative feedback suggests students enthusiastically endorse the self-directed active learning; they feel these exercises offer both a valuable approach to learning and an opportunity to practice presentation and leadership skills. First-year students enjoy the interaction with fourth-year facilitators and the fourth-year students appreciate the opportunity to review basic science material. Our data suggest that students learn to “think” about the anatomy, and we hope learn to use their understanding and knowledge base in a practical fashion. Moreover, these case-based exercises can fit nicely into a variety of curricular formats, especially where problem-based tutorials may not be feasible or desirable. © 1994 Wiley-Liss, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ca.980070608
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  • 6
    Electronic Resource
    Electronic Resource
    Morphology of the carotid sinus wall in normotensive and spontaneously hypertensive rats (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 218 (1987), S. 426-433 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The morphology of the carotid sinus region of the internal carotid artery was studied in spontaneously hypertensive rats (SHR) at 5, 8, 16, and 24 weeks of age. The carotid sinus region occupied the proximal millimeter of the internal artery, and was easily recognizable by the presence of an extensive adventitial capillary plexus, which was absent on adjacent arteries (e.g., common and external carotid arteries). Methylene blue-stained whole-mount preparations showed the extent of baroreceptor nerves over the sinus. Baroreceptor fibers terminated in distinctive bulbous-like endings, which, at the ultrastructural level, were filled with mitochondria. No differences were noted in the sinus adventitial capillary network or baroreceptor distribution between SHR and age-matched Wistar-Kyoto (WKY) normotensive control animals. With the onset of a significant rise in SHR blood pressure, the carotid sinus wall increased in thickness and total vessel size. The wall/lumen ratios were significantly larger in the SHR than in age-matched WKY ratios in all age groups. SHR carotid sinus vessel enlargement was uniform throughout the vessel tunics, with no significant change in the proportion of the tunica media occupied by smooth muscle cells. The increase in the carotid sinus wall thickness associated with increasing hypertension could affect the ability of the sinus to distend and may play a secondary role in the maintenance of hypertension by compromising baroreceptor nerve ending sensitivity.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092180410
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  • 7
    Electronic Resource
    Electronic Resource
    Localization of enkephalin-like immunoreactivity in the cat carotid and aortic body chemoreceptors (1982)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 203 (1982), S. 405-410 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The purpose of this study was to determine if enkephalin-like immunoreactivity was present in the glomus cells of the carotid and aortic body peripheral arterial chemoreceptors. Cat carotid and aortic bodies were reacted with antisera to met- and leu-enkephalin using the indirect peroxidase-antiperoxidase immunocytochemical method of Sternberger (1979). Both the carotid and aortic bodies demonstrated clusters of immunoreactive cells for both met- and leuenkephalin. Additionally, met-enkephalin-like immunoreactivity was observed in many of the dense-core vesicles of the glomus cells of the carotid body. The glomus cells of these chemoreceptors are known to contain catecholamines which may modulate chemoreceptor activity. The presence of opioid peptide-like substances co-existing with the glomus cell catecholamines, perhaps in the same vesicles, may have important implications for a trophic influence of these peptides on glomus cell chemoreceptor modulation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092030310
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of salt loading on the fractional volume of atria-specific granules in dahl salt-sensitive and salt-resistant rats (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 218 (1987), S. 157-161 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cardiac atria are known to play a role in blood volume homeostasis, secreting a peptide that induces a potent natriuresis and diuresis. This peptide is atrial natriuretic factor (ANF), and its primary site of storage is within atria-specific granules found in atrial cardiocytes.Since salt loading results in an increase in circulating levels of ANF, our aim was to determine if the atria-specific granule population in the cardiocytes of Dahl rats would decrease accordingly. To this end, the fractional volume of the atria-specific granules was determined by ultrastructural morphometric analysis in the Dahl salt model of hypertension. This analysis was performed on the right atria of Dahl salt-resistant (DR) and salt-sensitive (DS) rats fed either a low-salt (0.4%) or high-salt (8%) diet for 12 weeks prior to sacrifice. DR and DS rats fed a low-salt diet had significantly reduced plasma sodium levels and osmolalities, and a significantly lower mean arterial blood pressure than did rats fed a high-salt diet. The fractional volume of atria-specific granules was significantly lower in salt-loaded DR (P〈0.01) and DS (P〈0.025) rats than in their respective low-salt controls. This significant decrease in atrial granules corresponds to the reported decrease in the storage of atrial ANF in salt-loaded rats, and provides a morphological verification of the biochemical studies. Moreover, these results, in combination with a growing body of physiological data, lend support to the hypothesized role of ANF in the regulation of water-electrolyte balance, which may play an important role in cardiovascular pathophysiological states related to hypertension.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092180210
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  • 9
    Electronic Resource
    Electronic Resource
    Inverse correlation between “Synaptic” ribbon number and the density of adrenergic nerve endings in the pineal gland of various mammals (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 205 (1983), S. 93-99 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The number of “synaptic” ribbons was inversely correlated with the density of the adrenergic nerve endings of the pineal gland compared among a diverse group of species including the fox, cat, rat, cotton rat, white-footed mouse, Djungarian hamster, ground squirrel, and chipmunk. The concentration of norepinephrine paralleled the number of adrenergic nerve terminals in the pineal glands of the cotton rat, rat, and ground squirrel, the only species in which norepinephrine concentrations were measured. The number of ribbon fields paralleled numbers of “synaptic” ribbons in all species examined. Adrenergic nerve endings were observed primarily within the perivascular spaces, although some endings also were found among parenchymal cells. Adrenergic nerve endings forming synaptic junctions with pinealocytes were not observed in any of these species, nor was there any physical association between these nerve endings and “synaptic” ribbons.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092050112
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  • 10
    Electronic Resource
    Electronic Resource
    Presence of dense-core vesicles in pinealocytes of the cat (1982)
    Springer
    Cell & tissue research 222 (1982), S. 695-698 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Ultrastructure ; Dense-core vesicles ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dense-core vesicles, 90–200 nm in diameter, were found in pinealocytes of the cat. They were present both in perikarya and cell processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213868
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