ISSN:
1432-0983
Keywords:
Mutagen hyper-resistance
;
Yeast
;
Base sequence
;
Gene disruption
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A multi-copy plasmid containing the SNQ3 gene confers hyper-resistance to 4-nitroquinoline-N-oxide (4NQO), Trenimon, MNNG, cycloheximide, and to sulfometuron methyl in yeast transformants. Restriction analysis, subcloning, and DNA sequencing revealed an open reading frame of 1950 bp on the SNQ3-containing insert DNA. Gene disruption and transplacement into chromosomal DNA yielded 4NQO-sensitive null mutants which were also more sensitive than the wild-type to Trenimon, cycloheximide, sulfometuron methyl, and MNNG. Hydropathic analysis showed that the SNQ3-encoded protein is most likely not membrane-bound, while the codon bias index points to low expression of the gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00312733
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