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  • 1
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 91 (1987), S. 1900-1905 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 94 (1990), S. 2883-2889 
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0922-338X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1998
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract On radiographs flattening of the femoral head is evaluated by the Epiphyseal Index (EI). Using the ability of MRI to show articular cartilage and the physis clearly, we wish to propose the use of an Epiphyseal Index for MRI (EIM) and demonstrate the value in LCPD. Fifteen patients were examined using 1.5T MR scanner and T1-weighted coronal images were obtained. EIM was calculated as a ratio of height and width of cartilaginous contour surrounding epiphysis, and was compared between normal and the three radiographic stages. EIM of normal hips were ranged from 0.39 to 0.60 and had a tendency to decrease with increasing age. All cases of Stage 1 (avascular necrosis) were within the normal range (n=4, mean=0.51, SD=0.045). EIM of Stage 2 (fragmentation,n=15, mean=0.31, SD=0.055) were smaller than that of Stage 1. Stag 2 and 3 (residual,n=12, mean=0.31, SD=0.077) could not be distinguished by EIM. EIM was useful to show the flattening of epiphysis with growth and very important for differenciation between Stage 1 and 2 LCPD.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 72-76 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Culture conditions of Schizochytrium limacinum SR21 for the purpose of microbial docosahexaenoic acid (DHA) production were investigated. The strain SR21 showed a wide tolerance to salinity; that is, the optimum salinity was between 50% and 200% that of sea water. Monosaccharides (glucose and fructose) and glycerol supported good cell growth and DHA yield. Di- and polysaccharides, oleic acid, and linseed oil gave low DHA yields. A high content of DHA (more than 30% of total fatty acids) was obtained from culture on glucose, fructose, and glycerol, and also the strain had simple polyunsaturated fatty acid profiles. The major polyunsaturated fatty acids other than DHA were n-6 docosapentaenoic acid only, and the contents of icosapentaenoic acid and arachidonic acid were less than 1%. Using corn steep liquor as a nitrogen source, a high total fatty acid content was obtained. The total fatty acid content in the dry cell weight increased as the concentration of the nitrogen source decreased, reached more than 50%. An increase in carbon source concentration led to a high DHA yield. A maximum DHA yield of more than 4 g/l was obtained in both glucose and glycerol media at 9% and 12% respectively. S. limacinum SR21 was thought to be a promising resource for microbial DHA production yielding a good level of productivity as well as a simple polyunsaturated fatty acid profile.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth responses for bacteria culturable at 4 °C, isolated randomly from three different habitats comprising surface seawater, deep-sea water (5000 to 6000 m) and deep-sea sediments (ca. 2200 to 6000 m), were tested for growth at temperatures from 4 to 30 °C. All 67 isolates grew well at 4 and 20 °C, indicating that they were psychrotrophic. Compared with the surface seawater, psychrotrophic bacterial groups from the deep-sea were rich in bacteria with higher growth yield at 4 °C and lower doubling time at 30 °C. According to Ratkowsky's square root analysis, the deep-sea water group was characterized by lower predicted minimum growth temperature (mean Tmin = 266.2 K) than the surface-water group (269.9 K), while the lowest Tmin was measured in the deep-sea sediment group (263.9 K). Lower Tmin was also found in a deeper group among the sediment bacterial group. These data suggest that most of deep-sea bacterial isolates consist of members possessing higher adaptability to low-temperature environments. Psychrotrophs from deep-sea water also demonstrated an abundance of Gram-negative bacteria which were tentatively identified as belonging to the genus Moraxella, while psychrotrophs from surface water were more diverse. Bacillus spp. and Gram-positive coryneform bacteria dominated in deep-sea sediments, decreasing with increasing depth. Restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA of Moraxella-like bacteria showed that deep-sea strains were different from surface strains and from the four known species tested belonging to the Moraxella–Psychrobacter group.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Journal of oceanography 49 (1993), S. 353-367 
    ISSN: 1573-868X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract The plume of particulate DNA (0.7–1μg/l) was extended from a black smoker vent (c.a. 1340 m depth) to the west-southwest directions in the Izena bottom-water region. High concentrations of particulate materials (70–110 μg C/l; 300–570 μg S/l) were also detected in the bottom-waters. Microscopic observation showed that the bottom-waters were rich in alcian blue-stainable large amorphous particles which contained coccoid and rod-shaped microbial cells mostly smaller than 1μm. These microbial matrix compounds appeared to contribute to low P-DNA/P-C ratio (0.011±0.008;n=27) in the vent environment. Sulfur was detected in various kinds of particles in the waters, while the content varied with calcium. Microbial population in the P-DNA plume water was in the order of 105 cells/ml and the most (〉99.9%) were non-culturable. The composition of culturable heterotrophs differed between the bottom-waters and surface sediments surrounding the vent; contributions of low temperature (4°C)-culturable bacteria and manganese-oxidizing bacteria to the total heterotrophs were higher in the sediments than in the waters. In contrast, percentages of orange-pigmented heterotrophs and microorganisms capable of growing in thiosulfate- and ammonia-based media to the total heterotrophs were higher in the waters than in the sediments. These results suggest that the culturable bacterial community in the bottom-waters was nutritionally versatile. Izena hydrothermal activities seemed to have a great influence in concentrations and compositions of particulate mateirals and in biomass and compositions of microbial community in the vicinity of this aphotic deep-sea environment.
    Type of Medium: Electronic Resource
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