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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Anatomy and embryology 123 (1963), S. 490-497 
    ISSN: 1432-0568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In mice of an inbred Japanese dd-strain, age changes in the thymus weight were studied for both sexes from 5 days until 300 days of age. Further studies were made on effects of gonadectomy on the thymus in both male and female mice which were gonadectomized at 35 days and 100 days of age. 1. The thymus grows markedly at the same rate in both sexes in early life until at about 35 days it reaches a maximum in weight. Then it decreases in weight due to age involution. During adolescence and early maturity until about 100 days the decrease in thymus weight is relatively rapid, and it is more prominent in males than in females. During sexual maturity after 120 days until 300 days so far examined, the decrease in the thymus weight is very slow, and the thymus is significantly heavier in females than in males. 2. The sex difference in the thymus weight is no longer evident in gonadectomized mice. The thymus of the male responds to castration by a remarkable increase in weight, while the thymus of the female is little influenced by ovariectomy. From these considerations it is surmised that the sex difference in the thymus weight is due to that the thymus in the male is suppressed by the testis, namely, the male gonadal hormone.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Clinical Anatomy 8 (1995), S. 208-213 
    ISSN: 0897-3806
    Keywords: ligament ; joint capsule ; vein ; temporomandibular joint ; Life and Medical Sciences ; Miscellaneous Medical
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In the human temporomandibular joints of 14 Japanese cadavers, we observed a new retinacular ligament that connected to the retrodiskal pad posterolaterally and was accompanied by a vein draining the retrodiskal pad. This retinacular ligament originated from lateral to posterolateral part of the joint. The origin included the articular tubercle of the zygomatic process of the temporal bone. The ligament descended along the mandibular ramus to insert into the fascia of the masseter muscle at the mandibular angle. The vein originated from the venous plexus in the retrodiskal pad and ran parallel to the retinacular ligament, giving off branches to the retromandibular, transverse facial, and superfacial temporal veins. There were no remarkable differences in the size of the retinacular ligaments among individuals as well as between the left and right sides. The retinacular ligament had firm fibrous connections with the posterolateral part of the retrodiskal pad, where the external fibrous membrane of the articular capsule was lacking. These findings suggest that the retinacular ligament and its accompanying vein function together to maintain blood circulation during jaw movement and may also be related to the development of occlusal disorders. © 1995 WiIey-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 57 (1962), S. 667-678 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Influences of pregnancy and lactation on the thymus were quantitatively and microscopically studied in the mouse. Thytnic involution due to pregnancy occurs in later pregnancy and reaches a maximum at parturition. After parturition the involuted thymus regenerates, but lactation has an inhibitory influence on the regeneration. The possible significance of the thymic changes is discussed particularly in relation to the adrenocortical activity. Histologically the cortex exhibits prominent alterations during the involution and regeneration. The pattern of depletion and repopulation of lymphocytes in the cortex is similar to that in other types of acute involution. The medulla also undergoes microscopic changes which are revealed particularly by the histometric examination of its components.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 59 (1963), S. 513-529 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron microscopic studies have been made of the epithelial reticular cells of the thymus in mice of both sexes ranging in age from 5 to 8 weeks. The epithelial cells generally have long cytoplasmic processes by which they are interconnected and form a network throughout the organ. The processes adhere tightly to one another by desmosomes. At the surface of the organ the processes constitute a thin sheet, and a basement membrane is discernible close and parallel to the free surface of the epithelial sheet. In the cortex the meshes of the epithelial reticulum are filled with numerous lymphoid cells and relatively few mesenchymal reticular cells. The epithelial cells in the cortex are characterized by their slender cytoplasmic processes and by the presence of large round vesicles which contain coarsely granulated, dense material. By the presence of the vesicles as well as desmosomes at junctions of the cytoplasmic processes the epithelial cells can be distinguished from other cells. For comparison the cytological characteristics of the mesenchymal reticular cells are also described. In the medulla two types — reticular and hypertrophic — of epithelial cells are recognized. The cells of reticular type are irregularly stellated in shape with extended cytoplasmic processes. Their cytoplasm often contains considerable amounts of fine filaments in bundles. Due to the relative abundance of free ribonucleoprotein particles and other cytoplasmic components, the cytoplasm appears relatively electronopaque as compared with that of the cells of the other type. The plasma membrane of the cells of reticular type sometimes invaginates into the cytoplasm to enclose a lumen which contains substance of low density and sometimes fine filaments. A basement membrane-like layer is discernible close to the infolded plasma membrane in the lumen. The cells of hypertrophic type are relatively large and round with a few shorter cytoplasmic processes. They are characterized by the abundance of the smooth endoplasmic reticulum which appears as vesicle or sac of small size. These cells often possess peculiar vesicles the wall of which is provided with microvilli projecting into the lumen. Some of these vesicles carry cilia on their wall in addition to the microvilli. The cells of hypertrophic type often undergo degeneration. The degenerating cells are concentrically surrounded by a few neighboring cells of both hypertrophic and reticular types, and Hassall's corpuscles are formed.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 262 (1990), S. 199-201 
    ISSN: 1432-0878
    Keywords: Microfibrils ; Elastic fibers ; Reticular fibers ; Lymph node ; Tannic acid-uranyl acetate staining ; Mouse bgJ/bgJ-C57BL/6
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fibrous components other than collagen fibrils in the reticular fiber of mouse lymph node were studied by electron microscopy. Bundles of microfibrils not associated by elastin and single microfibrils dispersed among collagen fibrils were present. The diameter of the microfibrils was 13.29±2.43 nm (n=100). Elastin-associated microfibrils occurred at the periphery of the reticular fiber. Elastin was enclosed by microfibrils, thus forming the elastic fiber, which was clearly demonstrated by tannic acid-uranyl acetate staining. In the reticular fiber of lymph nodes, the elastic fiber consisted of many more microfibrils and a small amount of elastin. These microfibrils, together with the collagen fibrils, may contribut to the various functions of the reticular fibers.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 264 (1991), S. 215-219 
    ISSN: 1432-0878
    Keywords: Erythroblast ; Cytokinesis ; Cytoplasmic bridge ; Fetal liver ; Erythropoiesis ; Electron microscopy ; dd Mice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A unique cytoplasmic connection between erythroblasts was studied by electron microscopy in mouse hemopoietic tissues (fetal liver, fetal and neonatal spleen and adult bone marrow). Many pairs of interphase erythroblasts were connected by a “cytoplasmic bridge” that was very thin and sometimes long in comparison with telophase bridges. The stage of maturation of the cells in a pair was similar. Small numbers of microtubules ran along the cytoplasmic bridge; a mid-body was not seen. The plasma membrane at approximately the middle of the bridge bulged to form a ring-shaped ridge filled with dense amorphous substances; this was called a “bulging ring”. Thus, the cytoplasmic bridge between erythroblasts did not morphologically correspond to the telophase bridge in the usual cytokinesis. Cytoplasmic bridges were observed in various differentiating stages of erythroblasts, whereas other cell types of the hemopoietic lineage did not have such a bridge. The cytoplasmic bridge is unique to erythroblasts and provides an evidence for the atypical cytokinesis of the erythroblastic lineage.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 56 (1962), S. 445-464 
    ISSN: 1432-0878
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A histological study has been made of the thymus in mice during acute involution and regeneration following administration of hydrocortisone. The cortex undergoes remarkable changes in the microscopic structure during involution and regeneration. During involution the lymphocytes in the cortex rapidly decrease and are removed. Then a rapid replacement of lymphocytes occurs during regeneration. On the basis of formation and repopulation of lymphocytes the regenerative process of the cortex is divided into seven phases. The reconstitution of the cortex proceeds more rapidly in females than in males. Newly formed lymphocytes take origin from the mesenchymal cells in the cortex. Such mesenchymal cells become distinguishable from epithelial reticular cells during involution. They appear to engulf destroyed lymphocytes and debris during involution and then transform into immature lymphoid cells during early regeneration. The findings may support the recent reutilization concept that destroyed lymphocytes are phagocytized and reutilized by reticular cells in heteroplastic differentiation into immature lymphoid cells. In the cortex PAS-positive sudanophilic cells which are derived from the perivascular and subcapsular connective tissue appear with involutionary changes. They become gradually reduced again with progress of the regeneration of the cortex. During involution the medulla are temporarily filled with lymphocytes migrated from the cortex. The epithelial reticular cells in the medulla are found grouped in cords or clumps in the severely involuted thymus. In the medulla there are two types of PAS-positive epithelial reticular cells; one contains a large, colloid-like, PAS-positive inclusion within the cytoplasm and the other has cytoplasm diffusely filled with PAS-positive substance. During involution and early regeneration, the former type increases while the other shows almost no significant changes. Hassall's corpuscles somewhat increase in frequency during involution and early regeneration.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 271 (1993), S. 103-106 
    ISSN: 1432-0878
    Keywords: Skin ; Langerhans cell ; ATPase-histochemistry ; Ultrastructure ; Ia antigen ; Four avian species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The occurrence of cells resembling mammalian Langerhans cells in the avian epidermis was studied by ATPase histochemistry, Ia immunoreactivity and electron microscopy. The existence of MHC class II antigen-(Ia) expressing, ATPase-positive dendritic cells, which are ultrastructurally similar to mammalian Langerhans cells except for the absence of Birbeck granules, was demonstrated. These cells may be a basic component of the immune system of birds.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 164 (1969), S. 47-65 
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Thymic rudiments from mouse embryos varying in gestational from 10-13 days were examined by light and electron microscopy in an effort to learn the origin of thymic lymphocytes. Lymphocytes were recognized and distinguished from mesenchymal cells and thymic epithelial cells by their round shape, larger nucleoli, and high concentration of cytoplasmic ribosomes and dearth of endoplasmic reticulum. No lymphocytes could be found at the earliest stage of development of the third pharyngeal pouch  -  at approximately ten days' gestational age. They first appeared in the mesenchyme surrounding the third pouch at a later stage, (approximately 11 days' gestation), but in most cases did not appear in the thymic epithelium until the parathyroid and thymus began to differentiate at approximately 12 days' gestation. No cells were seen that appeared to be transitional between lymphocytes and epithelial or mesenchymal cells and it was concluded that these observations support the hypothesis that lymphocytes first reach the thymic parenchyma by immigration from the surrounding mesenchyme. Most of the lymphoctyes found in the region of the thymus at these stages of development were large lymphocytes. The origin of these early lymphocytes remains unknown.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0003-276X
    Keywords: Type VI collagen ; Mouse ; Masseter tendon ; Electron microscopy ; ATP treatment ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background and Methods: The association of masseter tendon type VI collagen with other extracellular matrix (ECM) components was examined from osseous attachment to myotendinous junction by immunohistochemistry and transmission electron microscopy with ATP treatment and enzyme digestion.Results: In the tendon proper, fibrocytes extended their processes among bundles of striated collagen fibrils and associated with adjacent cells through amorphous materials, thus forming a three-dimensional network. The amorphous or filamentous material was observed around the fibrocyte cell body and along the cell processes, where the localization of type VI collagen was confirmed by immunohistochemistry using anti-type VI collagen antibody. After treatment with 20 mM adenosine 5′-triphosphate (ATP), 100 nm periodic fibrils, an aggregated form of type VI collagen, were formed in the place where amorphous or filamentous material was present before the treatment. In myotendinous junction, the ATP-aggregated periodic fibrils were observed to associate with the external lamina of the muscle cells as well as among junctional tendon collagen fibrils. In the tendonbone boundary, ATP-aggregated periodic fibrils were observed around fibrocartilage-like cells in the uncalcifying area but not in the calcification front. Prolonged ATP treatment or hyaluronidase predigestion caused the formation of type VI collagen periodic fibrils in the area near the calcified matrix.Conclusions: The distribution of type VI collagen in mouse masseter tendon is different in different anatomical position. This may reflect the different functional demand for this collagen. © 1995 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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