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Mainly unmutated VH genes rearranged in B cells forming germinal centers in a cutaneous pleomorphic T-cell lymphoma (1999)

Golembowski, Sven ; Gellrich, Sylke ; Lorenz, Pamela ; [et al.]

Oxford, UK : Blackwell Publishing Ltd

Journal of cutaneous pathology 26 (1999), S. 0

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ISSN: 1600-0560

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: B Cells in skin lesions of a pleomorphic cutaneous T-cell lymphoma with reactive germinal center hyperplasia were analyzed for their immunoglobulin VHDJH gene rearrangements by micromanipulation and single cell polymerase chain reaction (PCR) analysis. In B lymphocytes located in germinal center-like structures, we found in 11/16 different VHDJH rearrangements completely Unmutaied VH genes, suggesting that those cells did not undergo antigen-driven selection. Two VH genes showed more than 98% germ-line identity. In only three cells VH segments were somatically mutated to a higher extent, but two of these rearrangements were non-productive. These results diller markedly from what we have previously detected in B cells present in mycosis fungoides, another entity of cutaneous T-cell lymphomas where the Ig gene repertoire resembles the situation in peripheral blood with a significantly higher proportion of mutated VH genes. When investigating the large atypical B cells strongly expressing CD30 which were detected within the T-cell zone outside the germinal centers. we found again, in most cases, that the rearranged VH genes were completely unmutated. The B cells were of polyclonal origin. Due to this comparable Ig gene repertoire and mutational pattern, we suggest that these cells descend from the germinal center centroblasts, which migrated into the T-cell zone and obviously became stimulated to express the CD30 marker. The mieromanipulation technique and molecular analysis on the single cell level may provide an important Input into our understanding of the mechanisms of immune regulation in cutaneous lymphomas.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1600-0560.1999.tb01783.x

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Immunochemical quantification of Cu/Zn superoxide dismutase in prenatal diagnosis of Down's syndrome (1990)

Porstmann, Tomas ; Wietschke, Roselotte ; Cobet, Günther ; [et al.]

Springer

Human genetics 〈Berlin〉 85 (1990), S. 362-366

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ISSN: 1432-1203

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Cu/Zn Superoxide dismutase (SOD) was quantified by enzyme immunoassay for prenatal diagnosis of Down's syndrome. Overall, 154 samples of amniotic fluid, 72 samples of amniotic cells and 31 samples of chorionic tissue were investigated. Due to the large biological variance of the SOD concentrations in normal pregnancies (range for amniotic fluid 10.5–154.9, for amniotic cells 40.0–338.8, and for chorionic tissue 132.2–649.5 g SOD/g protein) the cases of Down's syndrome detected by karyotype analysis were not reliably identified by Cu/Zn SOD quantification. As in erythrocytes obtained from patients with Down's syndrome, a trisomy 21 was easily and accurately detected in the erythrocytes from very small quantities (about 50 μl) of umbilical blood. The SOD concentrations in normal cases (n = 40) varied between 11.4 and 17.3 and in the cases of trisomy 21, as confirmed by karyotyping (n = 4), between 22.5 and 23.2ng/one million cells. SOD quantification in fetal erythrocyte is a helpful additional method in prenatal Down syndrome diagnosis under certain conditions, which are discussed.