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  • 1
    Electronic Resource
    Electronic Resource
    Plasmatubules in the pollen tubes of Nicotiana sylvestris (1988)
    Springer
    Planta 173 (1988), S. 35-41 
    Details
    ISSN: 1432-2048
    Keywords: Membrane extension ; Nicotiana (plasmatubules) ; Nutrient uptake (pollen tube) ; Plasma membrane ; Plasmatubule ; Pollen tube
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ultrastructural studies of the pollen tubes of Nicotiana sylvestris grown in the pistil revealed an extensive development of plasmatubules formed by evaginations of the plasma membrane. The plasmatubules occurred as twisted tubular structures in the periplasmic space along the tube wall and, in cross section, exhibited circular profiles with an outer diameter of 28±4 nm. They were also seen in deep, pocket-like invaginations of the plasma membrane and in this case the profiles had an outer diameter of 34±8 nm. In the pocket-like invaginations they were partially branched and often closely packed to form groups with obvious patterns. The enlargement of the plasma-membrane area resulting from plasmatubules formed along the tube wall was about six-to tenfold. Pollen tubes grown in vitro exhibited poorly developed plasmatubules. It is suggested that the large extension of the plasma membrane could enhance the uptake of nutrients, and thus might be responsible for the comparatively fast growth of pollen tubes in the pistil. Moreover, it is also assumed that the turnover rate of the Golgi apparatus must be higher in pollen tubes growing in vivo than in vitro, in order to provide a sufficient amount of membrane for the formation of the plasma membrane with its tubular modifications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394484
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of S-locus glycoprotein genes from Brassica oleracea and B. campestris in transgenic plants of self-compatible B. napus cv Westar (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 101-109 
    Details
    ISSN: 1432-2145
    Keywords: Self-incompatibility ; S-locus genes ; Brassica napus ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Self-compatible Brassica napus var ‘Westar’ was transformed with SLG, the S-locus-derived gene that encodes S-locus-specific glycoproteins (SLSG). Four allelic variants of SLG isolated from self-incompatible B. oleracea and B. campestris strains homozygous for different S alleles were used. We show that the transgenic plants synthesized SLSG with the same apparent charge, molecular weight, and antigenic properties as that produced by the corresponding self-incompatible strains from which the cloned SLG genes were isolated. In addition, transgene-encoded SLSG was detected specifically in the papillar cells of the stigma, and was correctly targeted to the papillar cell wall. However, SLSG was produced at reduced levels in transgenic plants relative to self-incompatible strains. The introduction of the SLG genes did not confer a self-incompatibility phenotype on the ‘Westar’ cultivar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194868
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructural responses of tobacco pollen tubes to heat shock (1989)
    Springer
    Protoplasma 153 (1989), S. 104-110 
    Details
    ISSN: 1615-6102
    Keywords: Nicotiana sylvestris ; Pollen tube growth ; Heat shock ; Ultrastructure ; Endoplasmic reticulum ; Mitochondria ; Golgi apparatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of elevated temperatures on semivivo growth and ultrastructure of tobacco pollen tubes was investigated. Tube growth was decreased by about 50% at 35 °C, independent of the duration of treatment, and at 40 °C and above there was no growth of tubes. Heat treatment caused ultrastructural changes like accumulation of membranous materials, concentric stacking of rough endoplasmic reticulum, reduction in vesicle production by dictyosomes, increase in the fenestrated regions of the Golgi cisternae, swelling of mitochondrial saccules and increase in the electron density of the mitochondrial matrix. Furthermore, the dictyosomes of the treated tubes showed significant increase in the number of cisternae from 30 to 45 °C. The temperature induced changes were persistant at least for 24 h in 35 °C grown pollen tubes. The possible reasons for the tube growth inhibition are discussed on the basis of the ultrastructural alterations caused by elevated temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322470
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Pentachlorophenol affects mitochondria and induces formation of golgi apparatus-endoplasmic reticulum hybrids in tobacco pollen tubes (1987)
    Springer
    Protoplasma 141 (1987), S. 112-120 
    Details
    ISSN: 1615-6102
    Keywords: Nicotiana sylvestris ; Pollen tube growth ; Pentachlorophenol ; Golgi apparatus ; ER ; Mitochondria ; Semivivo technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The toxic effect of pentachlorophenol (PCP) on the growth and ultrastructure of tobacco pollen tubes was tested using a semivivo technique of tube culture. In this technique the pollen tubes were allowed to grow in the pistilin situ for 24 hr before they protruded from the cut end of the style and came into contact with the medium containing PCP. The inhibitory effect of different PCP concentrations was determined by measuring the length of tube bundles. The intracellular action of PCP was analysed by electron microscopy. This biocide caused four obvious alterations in the pollen tube ultrastructure: (1) swelling of the mitochondrial saccules; (2) enlargement of the dictyosomes by the increase of the cisternal diameter and the number of cisternae per stack; (3) formation of cup-shaped Golgi apparatus-endoplasmic reticulum hybrid structures (GER hybrids) showing continuities of ER and Golgi cisternae; (4) formation of stacked and/or concentric arrangements of rough ER cisternae. It is suggested that swelling of saccules was directly due to the uncoupling of oxidative phosphorylation whereas the changes of the endomembrane system were caused by energy depletion due to the inhibition of ATP synthesis. These changes are consistant with dynamic concepts of dictyosome and ER function when membrane formation exceeds membrane use in the production of secretory and transition vesicles. Thus, the enlargement of the dictyosomes and the formation of GER hybrids are thought to result from inhibition of budding of vesicles from the Golgi apparatus or from both the ER and the Golgi apparatus, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01272892
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    Paper (German National Licenses)
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