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  • 1
    Electronic Resource
    Electronic Resource
    FORESTED WETLANDS IN EASTERN CONNECTICUT: THEIR TRANSITION ZONES AND DELINEATION (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the American Water Resources Association 16 (1980), S. 0 
    Details
    ISSN: 1752-1688
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Geography
    Notes: : The delineation of inland wetlands requires close field examination of the biological and physical gradients (transition zones) between wetlands and bordering uplands. As part of a study on the detection and delineation of inland wetlands in eastern Connecticut by remote sensing techniques, this effort was designed to investigate vegetation distribution and composition and selected physical and chemical properties of the soils of wetland to upland transition zones in deciduous wetland forests. Field research was conducted during the growing season of 1975 within a test area consisting of the 45 mi2 Town of Mansfield, Connecticut. Changes in vegetation composition and structure, soil pH, and soil water content were determined along line transects extended over wetland to upland transition zones. Differences in soil pH occurred along the transects but were of such magnitude that they probably have little impact on plant distribution. There were significant changes in soil water content along the wetland to upland gradients. Discriminant analysis applied to statistical “index of abundance” data describing vegetation distribution among the various zones (wetland, transition, upland) showed which plant species best distinguish wetlands from uplands. Of the criteria studied, vegetation composition and distribution, soil water content, and relief are the most useful criteria for delineating deciduous wetland forests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1752-1688.1980.tb02386.x
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  • 2
    Electronic Resource
    Electronic Resource
    Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 57-64 
    Details
    ISSN: 1432-2242
    Keywords: Brassica oleracea ; Linkage map ; Restriction fragment length polymorphism ; Duplicate loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A detailed genetic linkage map of Brassica oleracea was constructed based on the segregation of 258 restriction fragment length polymorphism loci in a broccoli × cabbage F2 population. The genetic markers defined nine linkage groups, covering 820 recombination units. A majority of the informative genomic DNA probes hybridized to more than two restriction fragments in the F2 population. “Duplicate” sequences having restriction fragment length polymorphism were generally found to be unlinked for any given probe. Many of these duplicated loci were clustered non-randomly on certain pairs of linkage groups, and conservation of the relative linkage arrangement of the loci between linkage groups was observed. While these data support previous cytological evidence for the existence of duplicated regions and the evolution of B. oleracea from a lower chromosome number progenitor, no evidence was provided for the current existence of blocks of homoeology spanning entire pairs of linkage groups. The arrangement of the analyzed duplicated loci suggests that a fairly high degree of genetic rearrangement has occurred in the evolution of B. oleracea. Several probes used in this study were useful in detecting rearrangements between the B. oleracea accessions used as parents, indicating that genetic rearrangements have occurred in the relatively recent evolution of this species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224016
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic analysis of morphological variation in Brassica oleracea using molecular markers (1994)
    Springer
    Theoretical and applied genetics 87 (1994), S. 721-732 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism ; Brassica oleracea ; Quantitative trait loci ; Morphological variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cross between the open-pollinated Brassica oleracea cabbage cultivar ‘Wisconsin Golden Acre’ and the hybrid broccoli cultivar ‘Packman’ was used with molecular markers to investigate the genetic control of morphological variation. Twenty-two traits derived from leaf, stem, and flowering measurements were analyzed in 90 F2 individuals that were also classified for genotype by restriction fragment length polymorphism (RFLP) markers. Seventy-two RFLP loci, which covered the mapped genome at an average of 10 map-unit intervals on all nine linkage groups, were tested individually for associations to phenotypic measurements by single factor ANOVA, and markers with significant associations (P〈0.05) were used to develop multilocus models. These data were utilized to describe the location, parental contribution of alleles, magnitude of effect, and the gene action of trait loci. Single marker loci that were significantly associated (P〈0.05) with trait measurements accounted for 6.7–42.7% of the phenotypic variation. Multilocus models described as much as 60.1% of the phenotypic variation for a given trait. In some cases, different related traits had common marker-locus associations with similar gene action and genotypic class ranking. The numbers, action, and linkages, of genes controlling traits estimated with marker loci in this population corresponded to estimates based on classical genetic methods from other studies using similar, or similarly-wide, crosses. There was no evidence that genome duplication accounted for a significant portion of multiple genes controlling trait loci over the entire genome, but possible duplications of trait loci were identified for two regions with linked, duplicated marker loci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222898
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  • 4
    Electronic Resource
    Electronic Resource
    Assessment of the degree of restriction fragment length polymorphism in Brassica (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 833-840 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism ; Brassica campestris ; Brassica oleracea ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The feasibility of creating a restriction fragment length polymorphism (RFLP) linkage map in Brassica species was assessed by screening EcoRI-, HindIII-, or EcoRV-digested total genomic DNA from several accessions of B. campestris, B. oleracea, and B. napus using random genomic DNA clones from three Brassica libraries as hybridization probes. Differences in restriction fragment hybridization patterns occurred at frequencies of 95% for comparisons of accessions among species, 79% for comparisons of accessions among subspecies within species, and 70% for comparisons among accessions within subspecies. In addition, species differences in the level of hybridization were noted for some clones. The high degree of polymorphism found even among closely related Brassica accessions indicates that RFLP analysis will be a very useful tool in genetic, taxonomic, and evolutionary studies of the Brassica genus. Development of RFLP linkage maps is now in progress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00258042
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  • 5
    Electronic Resource
    Electronic Resource
    Quantitative trait analysis of fruit quality in cucumber: QTL detection, confirmation, and comparison with mating-design variation (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 53-61 
    Details
    ISSN: 1432-2242
    Keywords: Genetic map ; QTL ; Cucumis sativus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cross within C. sativus var. sativus (GY14 x P1432860) and molecular markers were used to determine the number, magnitudes of effect, and overall variation described for genes conditioning the quantitatively inherited traits of length, diameter, seed-cavity size, color, L/D (length/diameter), and S/D (seed-cavity size/diameter). QTL effects were detected with MAPMAKER/QTL using 100 F3 lines evaluated in a replicated field trial of two harvests over 2 years at one location. Multilocus models were constructed by fixing significant intervals and re-scanning using MAPMAKER/ QTL. Marker inclusion in multilocus models was compared to an ANOVA “backward elimination” procedure. Generally the same loci were associated with QTLs among the two methods of model construction. Heritabilities of individual QTLs were confirmed by analysis of related backcrosses (67 BC1P1 lines and 68 BC1 P2 lines). The majority of QTLs were confirmed in at least one backcross population. Pairs of backcrosses allowed overall additive variances and heritabilities to be calculated using a North Carolina Design III (NCIII design) and estimates were compared to overall variances attributable to markers. Heritability estimates using markers were comparable, but generally lower than additive variances estimated by co-variance relationships in the NCIII design. This suggests that neither the number nor the magnitude of QTL effects were overestimated. The utility of backcrosses to confirm individual QTLs and the overall variance described by QTLs is recommended to avoid false positives and over-estimation of effects. The number of QTLs, and/or the proportions of phenotypic variation described by markers and the mating design, agreed with previous reports of heritabilities employing similar germplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220858
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  • 6
    Electronic Resource
    Electronic Resource
    A comparative map of wild rice (Zizania palustris L. 2n=2x=30) (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 677-684 
    Details
    ISSN: 1432-2242
    Keywords: Key words RFLPs ; Synteny ; Oryza ; Oat ; Map
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We present the first genetic map of wild rice (Zizania palustris L., 2n=2x=30), a native aquatic grain of northern North America. The map is composed principally of previously mapped RFLP (restriction fragment length polymorphism) genetic markers from rice (Oryza sativa 2n=2x=24). The map is important as a foundation for genetic and crop improvement studies, as well as a reference for genome organization comparisons among Gramineae species. A comparative mapping approach with rice is especially useful because wild rice is grouped in the same subfamily, Oryzoideae, and no other mapping comparison has yet been made within the subfamily. As rice is the reference point for mapping and gene cloning in cereals, establishing a consensus map within the subfamily identifies conserved and unique regions. The genomes of wild rice and rice differ in total DNA content (wild rice has twice that of rice) and chromosome pairs (wild rice=15 versus rice=12). The wild rice linkage map reported herein consists of 121 RFLP markers on 16 linkage groups spanning 1805 cM. Two linkage groups consist of only two markers. Colinear markers were found representing all rice linkage groups except #12. The majority of rice loci mapped to colinearly arranged arrays in wild rice (92 of 118). Features of the map include duplication of portions of three rice linkage groups and three possible translocations. The map gives basic information on the composition of the wild rice genome and provides tools to assist in the domestication of this important food source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051530
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