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  • 1
    Electronic Resource
    Electronic Resource
    The formation of lithium aroylnickel carbonylates from the reactions of aryllithiums and nickel carbonyl
    Amsterdam : Elsevier
    Journal of Organometallic Chemistry 5 (1966), S. 305-312 
    Details
    ISSN: 0022-328X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0022-328X(00)83419-1
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Assessment of the degree of restriction fragment length polymorphism in Brassica (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 833-840 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism ; Brassica campestris ; Brassica oleracea ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The feasibility of creating a restriction fragment length polymorphism (RFLP) linkage map in Brassica species was assessed by screening EcoRI-, HindIII-, or EcoRV-digested total genomic DNA from several accessions of B. campestris, B. oleracea, and B. napus using random genomic DNA clones from three Brassica libraries as hybridization probes. Differences in restriction fragment hybridization patterns occurred at frequencies of 95% for comparisons of accessions among species, 79% for comparisons of accessions among subspecies within species, and 70% for comparisons among accessions within subspecies. In addition, species differences in the level of hybridization were noted for some clones. The high degree of polymorphism found even among closely related Brassica accessions indicates that RFLP analysis will be a very useful tool in genetic, taxonomic, and evolutionary studies of the Brassica genus. Development of RFLP linkage maps is now in progress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00258042
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  • 3
    Electronic Resource
    Electronic Resource
    Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 593-600 
    Details
    ISSN: 1432-2242
    Keywords: Brassica rapa ; Brassica oleracea ; Molecular taxonomy ; Phylogenetic tree ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Preliminary analysis using nuclear RFLPs provided evidence that subspecies within Brassica rapa originated from two different centers. One center is in Europe, represented by turnip and turnip rape from which the oilseed sarson was derived. A second center is in South China containing a variety of Chinese vegetables of which pak choi and narinosa seem to be the most ancient forms. Based on RFLP data, the accessions of B. oleracea examined could be divided into three distinct groups, represented by thousand head kale, broccoli and cabbage. Thousand head kale and Chinese kale appear to be the primitive types. Observations of parallel variation among subspecies of both species are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260914
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  • 4
    Electronic Resource
    Electronic Resource
    Brassica taxonomy based on nuclear restriction fragment length polymorphisms (RFLPs) (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 784-794 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Genome evolution ; Phylogenetic tree ; Molecular taxonomy ; Restriction fragment length polymorphisms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction fragment length polymorphisms (RFLPs) of nuclear DNAs have been used to explore the origin and evolution of the six cultivated Brassica species. Extensive RFLP variation was found at the species, subspecies and variety levels. Based on RFLP data from Brassica and related genera, a detailed phylogenetic tree was generated using the PAUP microcomputer program, which permits a quantitative analysis of the interrelationships among Brassica species. The results suggested that 1) B. nigra originated from one evolutionary pathway with Sinapis arvensis or a close relative as the likely progenitor, whereas B. campestris and B. oleracea came from another pathway with a possible common ancestor in wild B. oleracea or a closely related nine chromosome species; 2) the amphidiploid species B. napus and B. juncea have evolved through different combinations of the diploid morphotypes and thus polyphyletic origins may be a common mechanism for the natural occurrence of amphidiploids in Brassica; 3) the cytoplasm has played an important role in the nuclear genome evolution of amphidiploid species when the parental diploid species contain highly differentiated cytoplasms. A scheme for the origins of diploid and amphidiploid species is depicted based on evidence gathered from nuclear RFLP analysis, cpDNA RFLP analysis, cytogenetic studies and classical taxonomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265606
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  • 5
    Electronic Resource
    Electronic Resource
    A linkage map ofBrassica rapa (syn.campestris) based on restriction fragment length polymorphism loci (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 296-304 
    Details
    ISSN: 1432-2242
    Keywords: Brassica rapa ; Brassica campestris ; Restriction fragment length polymorphism ; Linkage map ; Duplicated loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A detailed linkage map ofB. rapa (syn.campestris) was constructed based on segregation of 280 restriction fragment length polymorphism loci, detected by using 188 genomic DNA clones as probes on DNAs from a F2 population of Chinese cabbage ‘MichihilF’בSpring broccoli.’ These genetic markers covered 1,850 centiMorgans (cM) and defined ten linkage groups, which equals the haploid chromosome number of this species. Extensive sequence duplication was evident by the detection of two or more segregating loci with each of 69 clones (36.7% of the total). Although some duplicated loci were randomly distributed throughout the genome, many had linkage arrangements that were conserved on different linkage groups, suggesting that large chromosome fragments were present in multiple copies. However, conservation in the linkage arrangement of duplicate loci throughout entire pairs of linkage groups was not observed. Single-copy loci were often found to be located within conserved duplicated regions, and linkage distances between some loci having conserved duplicated arrangements were substantially different between the duplicated regions. Structural rearrangements, such as insertions, deletions, and inversions or combinations of these events, seemed to be related to the alternations of map distances between duplicated loci and to the dispersal of duplicated chromosome fragments. These results suggest thatB. rapa has evolved in part by duplication of chromosomes or large chromosome fragments with subsequent structural rearrangements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02190615
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular taxonomy of Portuguese tronchuda cabbage and kale landraces using nuclear RFLPs (1991)
    Springer
    Euphytica 58 (1991), S. 221-229 
    Details
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; landrace group ; molecular markers ; numerical taxonomy ; Portuguese coles ; RFLPs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary RFLPs were used to study the genetic relationships of 31 Portuguese coles (Brassica oleracea L.) representing the different ecotypes or landraces grown in Portugal. Other cole crops such as Jersey kale, Chinese kales, common cabbages, broccolis and cauliflower, two nine-chromosome wild species, and one accession of turnip also were included to determine the evolutionary relationships of Portuguese coles to other related coles. Principal coordinates and cluster analyses were conducted using a package of computer programs and RFLP data from 55 nuclear DNA probes, detecting 291 polymorphic restriction fragments. The results showed that the 48 accessions clustered into five groups: (a) a dispersed group with turnip and the wild brassicas; (b) Chinese kales; (c) broccolis and cauliflower; (d) a disperse group including Algarve and Jersey kales, thousand head kale, cabbages, and savoy cabbage; (e) a large and compact group containing all Portuguese tronchuda cabbages and kales. This unique and closely related group containing Portuguese tronchuda cabbages and kales could be further divided into five subgroups corresponding to the major areas of cultivation. The phenetic groupings of Portuguese tronchuda cabbages and kales based on RFLP data corresponded more with their geographic origins (collecting sites) than with their morphological similarities. A mechanism involving geographic isolation and frequent intercrossing within local areas is proposed to explain genetic relationships among Portuguese tronchuda cabbages and kales.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025253
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