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  • 1
    Electronic Resource
    Electronic Resource
    Protein gene-product 9.5 in developing mouse circumvallate papilla: comparison with neuron-specific enolase and calcitonin gene-related peptide (1996)
    Springer
    Anatomy and embryology 194 (1996), S. 365-372 
    Details
    ISSN: 1432-0568
    Keywords: Ontogeny ; Circumvallate papilla ; Taste buds ; Innervation ; Protein gene-product 9.5 ; Neuron-specific enolase ; Calcitonin gene-related peptide ; Immunohistochemistry ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was made to investigate the ontogeny of protein gene-product 9.5 (PGP 9.5)-like immunoreactivity (-LI) in the developing mouse circumvallate papilla (CVP), and its distribution was compared to that of neuron-specific enolase (NSE) and calcitonin gene-related peptide (CGRP). In adult CVP, PGP 9.5-LI was observed in the subgemmal nerve plexus; some thin PGP 9.5-like immunoreactive (-IR) nerve fibers penetrated taste buds and apical epithelium. PGP 9.5-LI was also observed in the spindle-shaped cells in taste buds, and a small number of round- or oval-shaped ganglionic cells in the lamina propria. The distribution of NSE-LI was comparable to that of PGP 9.5-LI. CGRP-LI was observed in the nerve fibers only; distribution of CGRP-IR nerve fibers was similar to that of PGP 9.5-IR nerve fibers, although the number of CGRP-IR nerve fibers was smaller than that of PGP 9.5-IR nerve fibers. At least six developmental stages were defined with regard to the developmental changes in the distribution of PGP 9.5-LI from embryonic day (E) 12 to adulthood: Stage I (E12–13) — a dense nerve plexus of PGP 9.5-IR nerve fibers was detected in the lamina propria beneath the core of newly-formed papilla. Stage II (E14–16) — thin PGP 9.5-IR nerve fibers penetrated the apical epithelium, and a few round-shaped cells in the apical epithelium also displayed PGP 9.5-LI. Stage III (E17–18) — thin PGP 9.5-IR nerve fibers penetrated the inner lateral epithelium of the trench. Stage IV [Postnatal day (P) 0–3] many PGP 9.5-IR nerve fibers penetrated the outer lateral epithelium of the trench; later in this stage, taste buds appeared. Stage V (P5–10) — a small number of PGP 9.5 IR cells in the taste buds appeared, and their number increased gradually. Stage VI (PI4-adult) — the number of PGP 9.5-IR taste cells increased and reached the adult level, while the number of PGP 9.5-IR nerve fibers decreased. The development of NSE-LI was similar to that of PGP 9.5-LI. CGRP-IR nerve fibers were detected at E12 in the lamina propria, and the development of the intraepithelial CGRP-IR nerve fibers was similar to that of PGP 9.5-IR nerve fibers. The present results indicate that invasion by nerve fibers of the epithelium of lingual papillae occurs in a complex manner, and that these nerve fibers may participate in the formation of the taste buds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00198538
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of epidermal growth factor on osteoblastic cellsin vitro (1983)
    Springer
    Calcified tissue international 35 (1983), S. 542-548 
    Details
    ISSN: 1432-0827
    Keywords: Osteoblasts ; Epidermal growth factor ; Alkaline phosphatase ; Collagen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effect of epidermal growth factor (EGF) on clone MC3T3-El cells that have osteoblastic activity was examined by phase-contrast microscopy and electron microscopy; hydroxyproline content, collagen synthesis, collagen pattern, and alkaline phosphatase (ALP) activity were also determined. We found that EGF (0.4 ng/ml) transformed the cells from their normal polygonal shape to a spindle-like morphology by 8 h. This hormone also caused dose-related suppression of hydroxyproline content and ALP activity which was detectable 2 days and 1 day, respectively, after EGF addition. Indomethacin did not affect hydroxyproline content and ALP activity, suggesting that the effect of EGF on the cells may not be mediated by prostaglandins. Epidermal growth factor at concentrations of 2 to 50 ng/ml significantly decreased collagen synthesis in the cells, whereas protein synthesis was stimulated. Electron microscopy demonstrated that collagen fiber formation was also reduced by EGF; an immature type of fibril was observed compared with the typical cross-striated one in the controls. Moreover, the hormone treatment also resulted in the appearance of type III collagen in addition to the type I already present in the cells. These suppressive effects of EGF on MC3T3-El cellsin vitro suggest that this hormone may be involved in bone remodellingin vivo as well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02405091
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  • 3
    Electronic Resource
    Electronic Resource
    Dissecting Aneurysm of the Peripheral Posterior Inferior Cerebellar Artery (2000)
    Springer
    Acta neurochirurgica 142 (2000), S. 1151-1156 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Dissecting aneurysm; posterior inferior cerebellar artery; subarachnoid haemorrhage; anastomosis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary ¶ Dissecting aneurysms of intracranial posterior circulation have recently been shown to be less uncommon than previously thought. However, those involving the posterior inferior cerebellar artery (PICA) and not vertebral artery at all are extremely rare. We report here a case of a patient with a dissecting aneurysm of the lateral medullary segment of PICA which presented as subarachnoid haemorrhage. The aneurysm was treated by trapping surgery and the distant PICA was anastomosed to the occipital artery. The patient showed a slight ataxia immediately after surgery but recovered fully.  Recovery from immediately postoperative cerebellar symptoms due to intra-operative ischemia seemed to be due largely to recovery of flow in the region of cortical branches of PICA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010070044
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  • 4
    Electronic Resource
    Electronic Resource
    MIB1 immunopositivity is associated with rapid regrowth of pituitary adenomas (1997)
    Springer
    Acta neurochirurgica 139 (1997), S. 426-432 
    Details
    ISSN: 0942-0940
    Keywords: Pituitary adenomas ; rapid regrowth ; MIB1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Pituitary adenomas are generally regarded as benign tumours, but they may recur. We identified eight patients with pituitary adenomas that showed rapid regrowth within 2 years of initial surgery. We estimated the percentage of cells in each specimen that showed positive immunostaining for MIB1 (a novel anti-Ki-67) and compared the values to those of 40 adenomas that showed no regrowth. The mean MIB1 index for 40 adenomas that showed no evidence of regrowth was 0.19±0.06%. This was significantly (p〈0.0001) lower than that for adenomas that showed rapid regrowth (1.27±0.31%), based on the initial resected specimens. Immediately after detection of rapid regrowth and in adenomas that were resistant to bromocriptine or irradiation, the MIB1 index was always greater than 1.0%. Most patients with rapidly regrowing adenomas were well controlled by radiation therapy. Our results suggest that a MIB1 index greater than 1.0% may be a useful predictor of rapid regrowth of pituitary adenomas and may be useful for planning of therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01808879
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  • 5
    Electronic Resource
    Electronic Resource
    A Case of Non-Functioning Pituitary Adenoma Accompanied by a Large Cyst (1998)
    Springer
    Acta neurochirurgica 140 (1998), S. 293-294 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: CSF; large cyst; pituitary adenoma; magnetic resonance imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050100
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  • 6
    Electronic Resource
    Electronic Resource
    Secretion of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 by Meningiomas Detected by Cell Immunoblot Analysis (1999)
    Springer
    Acta neurochirurgica 141 (1999), S. 481-486 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Meningioma; matrix metalloproteinase-9; tissue inhibitor of metalloproteinase-1; cell immunoblot.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary  To identify features of meningiomas that infiltrate dura mater, and to examine the role Matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) secretion in meningiomas, a cell immunoblot assay study was performed in 20 meningiomas. MMP-9 secretion was detected in 20 (100%) meningiomas and was independent of histological features. TIMP-1 secretion was detected in 8 (40%) meningiomas. For a cell immunoblot volume which included approximately 1350 cells, there were 50.4±49.4 dots which showed immunoreactivity to MMP-9 and 2.0±4.2 which showed immunoreactivity to TIMP-1. The number of dots that showed immunoreactivity to MMP-9 was not significantly different between tumours with atypical and benign histological features. TIMP-1 secretion was found in only 8 (40%) specimens and the percentage of TIMP-1 secreting cells was significantly lower than that of MMP-9 secretion. Thus, we propose that meningiomas classified histologically as both atypical and benign have the potential to infiltrate dura mater.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050328
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  • 7
    Electronic Resource
    Electronic Resource
    Transsphenoidal “Cross Court” Approach Using a Slightly Modified Speculum to Reach Pituitary Adenomas with Lateral Growth (2000)
    Springer
    Acta neurochirurgica 142 (2000), S. 1055-1058 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Cavernous sinus; pituitary adenoma; lateral growth; transsphenoidal surgery.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary ¶ Background. The purpose of this study was to evaluate the usefulness of an oblique “cross court” transsphenoidal approach using a speculum with slightly modified projections for pituitary adenomas showing lateral extension.  Method. The projection of the speculum on the side of the cavernous lesion was shortened by 5 mm. The tip of the longer projection was placed in the sphenoid sinus, while the end of the shorter projection was placed on the concha sphenoidalis. An oblique transsphenoidal view was then easily obtained by opening the speculum. The contralateral nasal incisura had to be widened in some cases to achieve a more oblique view.  Findings. Twenty-eight patients diagnosed with pituitary adenoma showing lateral growth underwent adenomectomy under direct observation of the medial portion of the cavernous sinus using this approach. Postoperative normalization of hyperendocrinopathy or gross total removal of the tumour was achieved in the majority of patients.  Interpretation. An oblique transsphenoidal approach using this speculum and contralateral minor maxillary osteotomy afforded a direct view of the medial cavernous sinus, sufficient for the removal of adenomas showing minor to moderate lateral growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010070062
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  • 8
    Electronic Resource
    Electronic Resource
    Matrix metalloproteinase-9 secretion by human pituitary adenomas detected by cell immunoblot analysis (1996)
    Springer
    Acta neurochirurgica 138 (1996), S. 1442-1448 
    Details
    ISSN: 0942-0940
    Keywords: Matrix metalloproteinase-9 ; pituitary adenomas ; cavernous sinus invasion ; cell immunoblot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Twenty-two pituitary adenomas were examined on the secretion of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) using a cell immunoblot assay, and discussed regarding an association between cavernous sinus invasion and the secretion of these proteins. The cell immunoblot assay, a kind of immunoblot procedure, is able to detect proteins at the single cell level and to detect the incidence of tumour cells secreting the target proteins in the total tumour cell population. The incidence of tumour cells secreting MMP-9 was significantly higher in invasive adenomas than in noninvasive ones. On the other hand, TIMP-1 secretion was not detected in any adenomas in this study. This result suggested that MMP-9 secretion, and especially the number of MMP-9-secreting cells, may be associated with cavernous sinus invasion of pituitary adenomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01411124
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  • 9
    Electronic Resource
    Electronic Resource
    Demonstration of the Optic Pathway in Sellar/Juxtasellar Tumours with Visual Disturbance on MR Imaging (1998)
    Springer
    Acta neurochirurgica 140 (1998), S. 541-548 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Optic pathway; sella turcica; MR studies; brain tumour; visual disturbance.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary  Objective. To prospectively compare the demonstration of the intracranial optic pathway in patients with sellar/juxtasellar tumours and with clinical evidence of visual disturbance using either spoiled gradient recalled acquisition in steady state (SPGR) or conventional spin echo (CSE) T1-weighted imaging.  Materials and Methods. We studied 108 patients with sellar/juxtasellar tumours presenting visual disturbance. Visualization of the optic pathway (nerves, chiasm, tracts) was compared between CSE T1-weighted coronal image and SPGR coronal image. In 18 patients, SPGR imaging was performed before and after administration of Gd-DTPA and visualization of the optic pathway was compared.  Results. On CSE T1-weighted coronal images of 108 patients with visual disturbance, the rates for visualization of the optic nerves, chiasms and tracts were 50%, 77.8% and 89.8% respectively. In contrast, on SPGR coronal image the rates were 80.6%, 96.3% and 92.6% respectively. The rates of visualization of the optic pathway were greater in non-enhanced that in those with enhancement. The rates of visualization in patients with recurrent tumours were less than those in patients with primary tumours. The rate of visualization of optic nerves in patients with meningioma was less than in patients with pituitary adenoma, craniopharyngioma or Rathke cleft cyst.  Conclusion. The rate of visualization of optic pathway structures on SPGR imaging (without enhancement) is greater than that on CSE T1-weighted imaging. It is important to understand the accurate position of the optic pathways with using new MR modality especially in surgical planning for lesions around the sella turcica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050138
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  • 10
    Electronic Resource
    Electronic Resource
    The function of the hypothalamo-pituitary axis in brain dead patients (1993)
    Springer
    Acta neurochirurgica 123 (1993), S. 64-75 
    Details
    ISSN: 0942-0940
    Keywords: Brain death ; hypothalamic hormone ; pituitary hormone ; cerebral blood flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to find out the function of the hypothalamo-pituitary axis in brain dead patients, pituitary and hypothalamic hormone concentrations were measured and several anterior pituitary releasing tests were carried out in 39 brain dead patients. In addition, cerebral blood flow measurements were simultaneously performed. In almost all cases, the blood concentration of pituitary and hypothalamic hormones were above the sensitivity of the assay. Anterior pituitary releasing tests indicated that efficient functions of the hypothalamus were severely suppressed, while the normal secretory mechanism of the anterior pituitary was partially preserved in brain dead patients. Histological changes of hypothalamic neurons varied from barely detectable ghost cells to nearly normal cells even in the same case. Although, the remaining circulation seemed not to be sufficient enough to maintain integrated hypothalamo-pituitary function, as shown by the examinations of cerebral blood flow, the presence of hypothalamic hormones in the systemic circulation suggests that these hormones were released and carried from the hypothalamus by minimal flow which is preserved even after the diagnosis of brain death.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476288
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