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  • 1
    Electronic Resource
    Electronic Resource
    Expression of α,βA and βB subunits of inhibin or activin and follistatin in rat pancreatic islets
    Amsterdam : Elsevier
    FEBS Letters 319 (1993), S. 217-220 
    Details
    ISSN: 0014-5793
    Keywords: Activin ; Follistatin ; Inhibin ; Islet of Langerhans Rat pancreas
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)80549-A
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  • 2
    Electronic Resource
    Electronic Resource
    An Ultrastructural Study of the Sertoli Cell in the Water Buffalo (Bubalus bubalis) (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 21 (1992), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The ultrastructure of Sertoli cell in the water buffalo (Bubalus bubalis) was observed in a transmission electron microscope. The nucleus had homogeneous nucleoplasm, scarce heterochro-matin and multivesicular nuclear body (MNB). The MNB was composed of numerous vesicles and ribosome-like dense structures. The vesicles varied in size and number and contained a sparse and flocculent substance. In the indentation of the nucleus, aggregates of ribosomes were frequently observed. In the apical and middle region of the cell, long mitochondria and microtubules were distributed parallel to the long axis of the cell. Non-laminated smooth ER and some ribosomes were also recognizable throughout this region. In the basal region, widely-distributed laminated smooth ER was characteristic. Microfilament bundles at ectoplasmic specialization were irregularly arranged. Frequently-emerged nodular processes occasionally separated from basal lamina and formed round structures within Sertoli cytoplasm. Although these characteristics of buffalo Sertoli cell were very similar to those of the bovine studied, the aggregate of ribosomes was more developed in the buffalo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1992.tb00323.x
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  • 3
    Electronic Resource
    Electronic Resource
    Production and Immunohistochemical Characterization of Monoclonal Antibodies Against Ovarian Antigen of the Common Tree Shrew (Tupaia glis) (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 27 (1998), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To study the ovarian function of the common tree shrew (Tupaia glis), monoclonal antibodies (MAb) against its ovarian antigen were produced. Several positive hybridomas were cloned and MAb-T2C9 (IgG) was examined for reactivity. Strong immunoreactivity of MAb-T2C9 was localized within the cytoplasm of luteal cells and in the theca interna cells of Graafian follicles. MAb-T2C9 reacted also with testicular interstitial cells and adrenocortical cells, except those of the zona glomerulosa. Similar reactions were seen in the goat and sheep. Western blotting analysis of the ovine corpus luteum after reaction with MAb-T2C9 revealed a single positive band of ≅60kDa. These findings suggest that MAb-T2C9 recognized protein molecules related to steroid synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1998.tb00156.x
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  • 4
    Electronic Resource
    Electronic Resource
    The Arterial Supply of the Male Reproductive System in the Hamster (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 17 (1988), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The major arteries, the testicular, deferential, prostatic, internal pudendal and external pudendal arteries, supply the male genital organs of the hamster. The same arterial patterns are found in three strains, except that the external pudendal artery arises from the external iliac artery or internal iliac artery in the APG strain, but only from the external iliac artery in the CBN and ACN strains. The deferential and prostatic arteries form the arterial loop on the dorsal surface of the ventrolateral lobe of the prostate. This loop may play some importance parts in the functional interaction between the epididymis and the prostate. The caudal epididymal artery arises more frequently from the spermatic cordai portion of the testicular artery than from the cranial epididymal artery, and it never arises directly from the abdominal part of the testicular artery as reported in the rat, mouse and rabbit. The deferential artery may join with the cranial vesicular artery to form the common trunk (umbilical artery), but sometimes these arteries arise directly from the internal iliac artery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1988.tb00568.x
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  • 5
    Electronic Resource
    Electronic Resource
    Testicular Dynamics in Syrian Hamsters Exposed to Both Short Photoperiod and Low Ambient Temperature (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The object of this study was to determine the details of morphological dynamics of spermatogenesis in Syrian hamsters exposed to both short photoperiod and low ambient temperature. Eight-week-old male hamsters, kept in a long photoperiod (14 h L, 10 h D), were transferred to a short photoperiod (6 h L, 18 h D) and kept there for 13 weeks to induce testicular regression. Some hamsters were then transferred from the room at 23°C to that at 5°C (5°C group). Remaining hamsters were continuously kept at 23°C (23°C group). Thereafter, the morphology was examined. As a result, it took only 8 weeks until spermatogenesis recovered in the 23°C group. However, it was not until 20 weeks that spermatogenesis was recognized in the 5°C group. As the regulation of seasonal testicular activity is characterized by coordinated shifts in the relationships among mitosis, meiosis, and apoptosis, the changes in the proliferative and apoptotic activities were examined. Although no significant difference in proliferative activity of spermatogonia between the 5°C and the 23°C groups was confirmed, a notable increase in the rate of apoptosis was observed in the 5°C group. Furthermore, this increase was more salient during the hibernation period. These findings suggest that both cold ambient temperature and hibernation caused the delay of testicular recrudescence and this delay arose from the increase of apoptotic activity but not the change in proliferative activity in spermatogonia in the 5°C group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2005.00599.x
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  • 6
    Electronic Resource
    Electronic Resource
    An Ultrastructural Study on the Leydig and Sertoli Cells in the Immature Lesser Mouse Deer (Tragulus javanicus) (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Leydig and Sertoli cells of the immature lesser mouse deer testes, obtained in East Malaysia, were observed using light and transmission electron microscopy (TEM). The testes were fixed in 5% glutaraldehyde, post-fixed in 1% OsO4, dehydrated in ethanol, and embedded in Araldite M. Serial semi-thin sections were cut, stained with toluidine blue and observed using light microscopy. Serial ultra-thin sections were cut, stained with uranyl acetate and lead citrate, and examined using TEM. As a result, ultrastructurally, two types of underdeveloped filament bundles were infrequently recognized in Leydig cells, but not in other testicular cells. One type was the underdeveloped bundles of actin filaments (approximately 5 nm in diameter), which were found in the nucleus of Leydig cells. The other type was the underdeveloped bundles of intermediate filaments (approximately 10 nm in diameter), which were found in the cytoplasm of Leydig cells. A multivesicular nuclear body (MNB) – specifically present in the Sertoli cell nucleus of ruminant testes – was infrequently observed. The MNB is situated in the vicinity of nuclear membrane, still in an underdeveloped stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2005.00589.x
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  • 7
    Electronic Resource
    Electronic Resource
    Histochemistry of Complex Carbohydrate in the Major Salivary Glands of Hoary Bamboo Rats (Rhizomys purinosus) (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 27 (1998), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The major salivary glands (parotid glands, monostomatic sublingual glands and submandibular glands) were obtained from hoary bamboo rats (Rhizomys purinosus) and fixed in Bouin's solution. Paraffin sections were subjected to a battery of staining methods including lectin staining for demonstration of complex carbohydrates. Among the three major salivary glands, unique histochemical features were observed in the submandibular gland. Different from most myomorpha species, submandibular glands of the hoary bamboo rats have two types of secretory cells in the secretory endpieces. One type of cells showed positive reactions with Alcian blue (AB)(pH2.5), periodic acid-Schiff (PAS) and some lectins (peanuts agglutinin, Griffonia simplicifolia I. Machura pomifera agglutinin). The granular ducts, which exist in animals belonging to suborder myomorpha, were not observed in the submandibular glands of this animal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1998.tb00172.x
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  • 8
    Electronic Resource
    Electronic Resource
    The Evidence of Carotid Body in the Carotid Rete of the Shiba Goat (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Anatomia, histologia, embryologia 23 (1994), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The carotid body was found in/on the cavernous sinus of the carotid rete of the Japanese miniature Shiba goat by light and electron microscopies. The carotid body-like aggregated cell group consisted of two types of cells arranged in the lobule. The first type contained membrane-bound granular vesicles; these cells resembled to the type-I cells in the carotid body. The second type contained no cytoplasmic vesicles, and were located in the periphery, investing the type-I cells. These features appear to be consistent with those of the type-II cells in the carotid body. The nerve ending showed two peculiar features in the intercellular space of type-I cells. The clear vesicle-containing nerve ending showed a structural feature indicative of efferent synapsis. The small granular vesicle-containing nerve ending has been described as a baroreceptor-like ending by VERNA (1979). All of these features are typical characteristics of the carotid body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.1994.tb00246.x
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  • 9
    Electronic Resource
    Electronic Resource
    Immunohistochemical Localization of Inhibin and Steroidogenic Enzymes in the Ovary of Common Tree Shrew (Tupaia glis) and Northern Smooth-tailed Tree Shrew (Dendrogale murina) (2000)
    Oxford, UK : Blackwell Science, Ltd
    Anatomia, histologia, embryologia 29 (2000), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: To study the ovarian function of the Order Scandentia, the localization of inhibin and steroidogenic enzymes (3β-hydroxysteroid dehydrogenase/isomerase and aromatase) in the ovaries of common tree shrew (Tupaia glis) and northern smooth-tailed tree shrew (Dendrogale murina) was immunohistochemically analysed. As in the results reported for other mammals, inhibin α-chain was localized in the follicular epithelium of secondary or Graafian follicles in the two species. The localization of aromatase in the ovary of these two species, however, was different. In the common tree shrew, the aromatase was localized in the thecal cells, whilst in other mammals it is localized in the granulosa cells. These results indicate that in the ovary of the common tree shrew, the oestradiol may be synthesized in the thecal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0264.2000.00277.x
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  • 10
    Electronic Resource
    Electronic Resource
    Peculiar Bundles of Filaments in Leydig Cells of the Lesser Mouse Deer (Tragulus javanicus): an Ultrastructural Study (2003)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 32 (2003), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Leydig cells of lesser mouse deer (Tragulus javanicus) testes were observed using light and transmission electron microscopies. Sexually mature lesser mouse deer were obtained in East Malaysia. The testes were perfused with 5% glutaraldehyde, postfixed with 1% OsO4, dehydrated in ethanol and embedded in Araldite. The semithin sections were cut, stained with toluidine blue and observed under light microscopy. The ultrathin sections were cut, stained with uranyl acetate and lead citrate, and examined using a JEM-1200 transmission electron microscope. As a result, two types of filament bundles were frequently recognized in Leydig cells, but not in other testicular cells. These bundles were clearly seen at even a light microscopic level. One type was bundles of actin filaments (approximately 5 nm in diameter). These structures were found not only in the cytoplasm but also in the nucleus. The other type was bundles of intermediate filaments (approximately 10 nm in diameter). These structures were found only in the cytoplasm. The existence of filament bundles has never been reported in the testicular cells of another mammalian species. Thus, while bundles of actin and intermediate filaments are specifically present in the Leydig cells of the lesser mouse deer, their functions are still unclear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2003.00499.x
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