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  • 1
    Electronic Resource
    Electronic Resource
    Limulus amebocyte lysate test for endotoxemia (1981)
    Springer
    Journal of molecular medicine 59 (1981), S. 213-218 
    Details
    ISSN: 1432-1440
    Keywords: Endotoxin ; Limulus test ; Leucocytosis ; Liver cirrhosis ; Septicemia ; Endotoxin ; Limulus Test ; Leukozytose ; Leberzirrhose ; Sepsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die fragliche Spezifität des Limulus Amebozyten Lysat (LAL) Testes bei der Diagnose der Endotoxinaemie stellt einen limitierenden Faktor für seinen klinischen Einsatz dar. Mit Hilfe eines Femtogramm-sensitiven spektrophotometrischen LAL Testes zeigten 35 von 36 septischen postoperativen Patienten eine gute Korrelation (fast 100%) zwischen positiven LAL Testen und Blutkultur positiven gram-negativen Bakteriaemien. 20 Patienten dieser Gruppe zeigten eine signifikante Korrelation (2p〈0.05) zwischen erhöhten Leukozytenzahlen und Escherichia coli Endotoxin Äquivalenten (EcEä). Bei 22 Patienten mit Leberzirrhose und einer potentiell enterogenen Endotoxinaemie konnten LAL positive Reaktionen (100%) nachgewiesen werden, aber 9 von 22 korrelierten signifikant (2p〈0.01) zwischen Leukozytose und erhöhten EcEä. Eine signifikante Korrelation zwischen EcEä und Leukozytenzahlen wurde für alle Neonaten und Schwangeren (2p〈0.001, 2p〈0.01) festgestellt. In vitro Tests zeigten, daß Leukozyten positive LAL Tests bewirkten. Überstände der mit Ficoll sedimentierten groben Leukozyten-Präparationen induzierten eine LAL Positivität in einer dosisabhängigen Form, während alle Reagentien per se, die für die Präparation gebraucht wurden, negativ waren. Diese Arbeit legt die Vermutung nahe, daß hitze-labile Faktoren von Leukozyten neben Endotoxin für positive LAL Reaktionen verantwortlich sind.
    Notes: Summary The question of specificity of Limulus amebocyte lysate (LAL) test in the diagnosis of endotoxemia has been a limiting factor of its clinical application. Using a femtogram-sensitive spectrophotometric LAL assay 35 of 36 septic postoperative patients showed an excellent correlation (almost 100%) between positive LAL tests and culture-proven gramnegative bacteremia. Twenty patients of this group demonstrated a significant correlation (2p〈0.05) between elevated total white blood cell counts and Escherichia coli Endotoxin equivalents (EcEe). All 22 liver cirrhotic patients with potentially enteric endotoxemia yielded LAL positive reactions (100%) but 9 of 22 correlated significantly as to leucocytosis and elevated EcEe (2p〈0.01). A significant correlation between EcEe and leucocyte counts was found for all neonates and all parturients respectively (2p〈0.001, 2p〈0.01). In vitro tests showed that leucocytes gave positive LAL tests. Supernates of a Ficoll sedimented crude leucocyte preparations, induced LAL positivity in a dose dependent manner, while all reagents per se used in the preparation, were negative. This study suggests that heat-labile factors from leucocytes apart from endotoxin are responsible for positive LAL reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476578
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  • 2
    Electronic Resource
    Electronic Resource
    Temperature effects of dissociative electron attachment to CF"3Cl
    Amsterdam : Elsevier
    Chemical Physics Letters 231 (1994), S. 460-466 
    Details
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-2614(94)01400-0
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  • 3
    Electronic Resource
    Electronic Resource
    Polanyi rules for ultrafast unimolecular reactions: simulations for HCo(CO)4(1E)* .fwdarw. hydrogen atom + cobalt tetracarbonyl (1993)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 97 (1993), S. 12485-12490 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100150a007
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  • 4
    Electronic Resource
    Electronic Resource
    Photodissociation Dynamics of Organometallic Complexes: Model Simulation for H + Co(CO)4 .rarw. HCo(CO)4 .fwdarw. HCo(CO)3 + CO (1994)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 98 (1994), S. 9823-9830 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100090a016
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  • 5
    Electronic Resource
    Electronic Resource
    Temperature determination of transient species by degenerate four wave mixing: Application of the independently determined power law of the transition dipole moment and geometric factors (1996)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 104 (1996), S. 9698-9703 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: We investigated the dependence of the degenerate four wave mixing (DFWM) signal intensities on the electronic transition dipole moment of isolated lines in the A 1Σ+←X 1Σ+ transition band of NaH. We applied a new method to determine this dependence in transient species without previous knowledge of the sample temperature. By using different appropriate pairs of DFWM lines sharing common lower level, the relative population difference is eliminated. We found that this ratio is well described by a power law (μ1/μ2)x where μi is the electronic one-photon transition dipole moment. As a result of saturation the exponent x depends on the total laser energy deposited in the medium. The observations are in good agreement with a modified two-level model, which includes the effects of polarization of the laser beams used in the experimental setup by introducing geometric factors. The exponent of the integrated signal intensities varies between 3 and 8 for high and low laser intensities with a rapid decrease to high intensities. This makes it possible to compare signal intensities at different DFWM laser intensities in order to obtain relative level populations, i.e., temperature, with high sensitivity. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.471732
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  • 6
    Electronic Resource
    Electronic Resource
    91. Die offene Peritonealspülung zur Behandlung der schweren Peritonitis (1980)
    Springer
    Langenbeck's archives of surgery 352 (1980), S. 323-323 
    Details
    ISSN: 1435-2451
    Keywords: Peritonitis ; Open treatment ; Irrigation-drainage ; Peritonitis ; Spülung ; Therapie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die offene Bauchwandstabilisierung, kombiniert mit Zulaufschläuchen in allen 4 Quadranten, ermöglicht eine kontinuierliche offene dorsoventrale Dauerspülung der Bauchhöhle bei der schweren Peritonitis. 10 Patienten mit fortgeschrittenen Stadien der Peritonitis wurden durchschnittlich 5 Tage mit ca. 401/24h Elektrolytlösungen gespült. 9 Patienten verstarben, Sektionsbefunde ergaben eine weitgehende Kontrolle der lokalen Keimbesiedelung. Die hohe Letalität ist bedingt durch sekundäre Organschäden der invasiven Sepsis.
    Notes: Summary Continuous open dorsoventral irrigation-drainage of the peritoneal cavity using copious amounts (401/24h) of elektrolyte solution was instituted in ten patients with severe and advanced peritonitis. Although nine patients died, autopsy revealed nearly complete local control of infection; the high mortality was due to secondary manifestations of invasive sepsis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01292052
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  • 7
    Electronic Resource
    Electronic Resource
    Altered potassium homeostasis in Crohn's disease (1983)
    Springer
    European journal of nuclear medicine 8 (1983), S. 245-249 
    Details
    ISSN: 1619-7089
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The total body potassium (TBK), serum potassium, and the number of red blood cell ouabain-binding sites was studied in 94 patients with Crohn's disease. TBK was measured by counting the endogenous 40K in a whole body counter. TBK was 87%±13% in 94 patients with Crohn's disease, while in control subjects, it was 97%±12% (n=24). This significant reduction in TBK was accompanied by normal serum potassium levels (4.4±0.5 mM). TBK was significantly correlated with the Crohn's disease activity index (r=0.79, n=113, P〈0.01). The number of red cell ouabain binding sites measured by equilibrium binding of 3H-ouabain showed a significant increase in the number of Na−K pumps in Crohn's disease (396±65, n=27) compared with the control group (290±45; n=24). These results support the suggestion that changes in TBK may regulate the synthesis of Na−K pump molecules. The total body potassium depletion and the need for a preoperative nutritional support in Crohn's disease are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00522514
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  • 8
    Electronic Resource
    Electronic Resource
    Obstruktion des linken Hemikolons: Subtotale Kolektomie, intraoperative Lavage oder Kolostomie? (1992)
    Springer
    Langenbeck's archives of surgery 377 (1992), S. 253-256 
    Details
    ISSN: 1435-2451
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210283
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  • 9
    Electronic Resource
    Electronic Resource
    Der postoperative Energiebedarf nach großen abdominal-chirurgischen Eingriffen: Vergleich der Messung durch indirekte Calorimetrie mit rechnerischer Schätzung (1989)
    Springer
    Langenbeck's archives of surgery 374 (1989), S. 138-145 
    Details
    ISSN: 1435-2451
    Keywords: Energy expenditure ; Abdominal surgery ; Indirect calorimetry ; Postoperative nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Nach großen abdominalchirurgischen Eingriffen ist der Energiebedarf frühoperativ um 30% and spätpostoperativ um 50%, bezogen auf den nach Harris-Benedict ermittelten basalen Energiebedarf, erhöht. Der Vergleich der indirekt calorimetrischen Meßwerte mit den Schätzwerten nach der Harris-Benedict-Formel zeigt für spätpostoperative Patienten eine gute Übereinstimmung (Korrelationskoeffizient r=0,76). Ähnlich genaue Angaben lassen sich auch mit einer einfachen Multiplikation des Körpergewichtes mit dem Faktor 30 erzielen. Eine aufgetretene Sepsis steigert den postoperativen Energiebedarf nicht erheblich, die Korrelation zwischen Messung und Schätzung ist jedoch gering. Eine einstiindige Meßdauer hat sich in unserer Meßserie als ausreichend genau erwiesen, wie auch der Vergleich mit Ergebnissen zeigt, die unter Verwendung von doppelt markiertem radioaktiven Wasser gemacht wurden.
    Notes: Summary Energy requirement after major abdominal operations, as calculated according to the formula for basic energy expenditure by Harris-Benedict, was increased by 30% in the early and by 50% in the late postoperative period. Correlation of these calculated values to measurements by indirect calorimetry was good. Even more simply a good estimation of caloric requirements can be obtained by multiplication of the body weight with a factor 30. The development of septic complications does not increase considerably postoperative energy expenditure, however, the correlation between measured and estimated values becomes poor. As supposed from isotope studies measuring gas exchange for indirect calorimetry for one hour provides sufficiently stable results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01261723
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  • 10
    Electronic Resource
    Electronic Resource
    Technik der laparoskopischen Cholezystektomie (1991)
    Springer
    Langenbeck's archives of surgery 376 (1991), S. 367-374 
    Details
    ISSN: 1435-2451
    Keywords: Laparoscopic cholecystectomy ; Surgical technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine Technik der laparoskopischen Cholezystektomie beschrieben, die in allen wesentlichen Operationsschritten der konventionellen Technik der offenen Cholezystektomie angeglichen ist. Das Operationsteam ist wie bei der offenen Technik positioniert. Der 1. Assistent führt die Videokamera, so daß der Operateur die eigentlichen operativen Schritte mit beiden Händen durchführen kann. Die Präparation erfolgt durch stumpfes Auseinanderdrängen der Infundibulumstrukturen und Abzupfen des Binde- und Fettgewebes von den Leitstrukturen A. cystica and Ductus cysticus stets in Richtung auf den Ductus choledochus. Das Auslösen der Gallenblase erfolgt durch Elektrokoagulation. Bei 178 Eingriffen, die von 8 Operateuren durchgeführt wurden, war die durchschnittliche Operationszeit 50 min. An Komplikationen traten 1 Fall von Zystikusstumpfinsuffizienz sowie ein intestinale Perforation auf; beide Fälle waren reoperationspflichtig. Ein 3. Fall von postoperativer Cholorrhoe heilte konservativ aus. Es trat kein Fall von Gallenwegsläsion auf. Die Umsteigerate betrug 2,5%. Die neue Methode erscheint hinreichend sicher und durch ihre Ähnlichkeit zur konventionellen Technik leicht erlernbar, so daß sie als Standardvorgehen empfohlen werden kann.
    Notes: Summary A new technique for laparoscopic cholecystectomy is described which regards as far as possible the conventional approach to cholecystectomy. Positioning of the operating team is identical to normal cholecystectomy, and the surgeon is able to use both hands to operate since the camera is handled by the assistant. Dissection of the infundibulum is performed in the socalled “tease-and-tear” technique. The peritoneal layer is opened by coagulation. The cystic duct and artery are bluntly dissected by a commercially available dissector. Fat and connective tissue are gently torn off from both structures. After closure of cystic duct and artery by clips, the gallbladder is cut out by thermocoagulation. 178 operations were performed by 8 surgeons; mean duration of the operation was 60 min. A change to open cholecystectomy was necessary in 2.5%. Three complications occurred, requiring reoperation in one case of insufficiency of the cystic duct and another one with intestinal perforation. In the third case, bile leakage from an aberrant bile-duct occurred but dried up spontaneously after a few days. No death occurred nor were there any lesions of the common bile-duct. Conclusively, this new technique seems to be safe and simple to teach due to its approximity to the conventional technique and is recommended as a standard procedure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186431
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