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  • 1
    Electronic Resource
    Electronic Resource
    X-ray titration of binding of β, γ-imido-ATP to myosin in insect flight muscle (1976)
    [s.l.] : Nature Publishing Group
    Nature 262 (1976), S. 613-615 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Unfortunately both structural signs can be faulted, the electron microscopy because of the possibility of fixation or postfixation changes6, and the X-ray diffraction because of the long time which was used to obtain the required exposures2,4. We have tried to overcome these objections by observing ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/262613a0
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  • 2
    Electronic Resource
    Electronic Resource
    The extracellular matrix proteins laminin and fibronectin modify the AMPase activity of 5'-nucleotidase from chicken gizzard smooth muscle
    Amsterdam : Elsevier
    FEBS Letters 195 (1986), S. 82-86 
    Details
    ISSN: 0014-5793
    Keywords: 5'-Nucleotidase Laminin Fibronectin Actin Laminin receptor
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(86)80135-1
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  • 3
    Electronic Resource
    Electronic Resource
    5'-Nucleotidase is involved in chick embryo myoblast spreading on laminin
    Amsterdam : Elsevier
    Cell Biology International Reports 14 (1990), S. 155-164 
    Details
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0309-1651(90)90032-T
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  • 4
    Electronic Resource
    Electronic Resource
    Structure of gelsolin segment 1-actin complex and the mechanism of filament severing (1993)
    [s.l.] : Nature Publishing Group
    Nature 364 (1993), S. 685-692 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The structure of the segment 1 domain of gelsolin, a protein that fragments actin filaments in cells, is reported in complex with actin. Segment 1 binds monomer using an apolar patch rimmed by hydrogen bonds in a cleft between actin domains. On the actin filament model it binds tangentially, ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/364685a0
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  • 5
    Electronic Resource
    Electronic Resource
    Absence of myosin-like immunoreactivity in stereocilia of cochlear hair cells (1982)
    [s.l.] : Nature Publishing Group
    Nature 300 (1982), S. 531-532 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 a, 0.1% SDS-(10%) polyacrylamide gel electrophoresis of calf thymus myosin which was further purified by DEAE-cellulose ion-exchange chromatography and used to prepare antibodies. HC, myosin heavy chain; 20K LC, 20,000-Mr myosin light chain; 16K LC, 16,000-A/r myosin light chain, b, Double ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/300531a0
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  • 6
    Electronic Resource
    Electronic Resource
    Distribution of deoxyribonuclease I (DNase I) and p53 in rat testis and their correlation with apoptosis (1996)
    Springer
    Histochemistry and cell biology 106 (1996), S. 383-393 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The testis is a tissue of high proliferative activity. In this organ, sperm cells (spermatozoa) are produced from stem cells (spermatogonia) by two consecutive steps of cell multiplication and spermatid cytodifferentiation. Mitotic proliferation of spermatogonia generates primary spermatocytes which enter meiosis, leading to the generation of spermatids. The number of cells entering meiosis is held constant, since outnumbering spermatogonia or premeiotic spermatocytes are eliminated by apoptosis (programmed cell death). During apoptosis, the nuclear chromatin is internucleosomally degraded by the activity of a Ca2+, Mg2+-dependent endonuclease. Recent data indicate that deoxyribonuclease I (DNase I) is identical to the apoptotic endonuclease responsible for the internucleosomal DNA degradation. Previous results using primers specific for rat parotid DNase I in a polymerase chain reaction have demonstrated the presence of DNase I-specific gene transcripts in rat testis. We have therefore analysed the presence of DNase I in rat testis by immunohistochemistry and biochemical procedures. The presence of DNase I-like endonucleolytic activity was verified enzymatically. DNase I immunoreactivity was detected in the nuclei of a few spermatogonia and premeiotic spermatocytes, but within the acrosomic vesicle of all spermatids and spermatozoa. In situ hybridisation revealed the accumulation of DNase I-specific gene transcripts in a small number of spermatogonia and/or premeiotic spermatocytes, but in a large number of spermatids. The occurrence of apoptotic DNA fragmentation was investigated by in situ end-labelling (ISEL) of free 3′-OH DNA ends and gave positive nuclear staining of only very few spermatogonia. No positive ISEL staining was observed in maturing spermatids and/or spermatozoa. These data support the notion that, within the seminiferous epithelium, the number of primary spermatocytes entering meiosis is controlled by apoptosis. In addition, they demonstrated that mature sperm cells are equipped with an endonuclease that might be used for DNA degradation during their elimination at later stages of their life span. The expression and distribution of the tumour suppressor gene product, p53, was analysed by immunostaining. Strong p53 immunoreactivity was observed in the nuclei of a number of spermatogonia, of some premeiotic spermatocytes and probably in all spermatids. Thus, p53 expression appeared to parallel that of DNase I. In contrast, p53 immunoreactivity was absent in mature spermatozoa present in the lumen of the testicular tubules or the ductus epididymidis. It is therefore proposed that at later stages of spermatid maturation – most probably before their release as mature spermatozoa – the p53 gene product was either degraded or retained in residual bodies, since p53 immunoreactivity was found to be concentrated within these organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050055
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  • 7
    Electronic Resource
    Electronic Resource
    Activation by ADP and the correlation between tension and ATPase activity in insect fibrillar muscle (1970)
    Springer
    Pflügers Archiv 321 (1970), S. 223-232 
    Details
    ISSN: 1432-2013
    Keywords: Muscle Contraction ; Adenosine Triphosphatase ; Adenosine Diphosphate ; Temperature ; Glycerol Extracted Insect Muscle ; Muskelkontraktion ; Adenosinetriphosphatase ; Adenosine-diphosphat ; Temperatur ; Glycerolextrahierter Insektmuskel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect of ADP on the tension and ATPase activity of glycerinated fibres of the flight muscles of the giant water bugLethocerus cordofanus has been investigated. Both increased, by a variable amount, when the ADP concentration was raised to 1 mM, but the increase was reversed when the concentration was raised further. In another series of experiments, the effect of temperature on the ATPase activity and tension was measured. There was a positive correlation between ATPase activity and tension in both types of experiment, the slopes being similar to those reported by other workers when calcium ion concentration or mean muscle length were altered. The positive feedback resulting from the increase of ATPase activity caused by ADP explains at least in part the variability of the results, and provides a possible explanation of the “high tension state” in glycerinated fibrillar muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00588443
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  • 8
    Electronic Resource
    Electronic Resource
    Basal cells of H-Dunning tumor are myoepithelial cells (1991)
    Springer
    Histochemistry and cell biology 95 (1991), S. 341-349 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Recent immunohistochemical studies have shown that basal cells in human prostatic epithelium are not myoepithelial cells. Since in the literature the Dunning tumor, originally described as a rat prostate carcinoma derived from the dorsolateral prostate of a Copenhagen rat, was reported to have myoepithelial cells, a comparative immunohistochemical and ultrastructural study was performed in the H-, HIF- and AT3-lines of the Dunning tumor, the male accessory sex glands (ventral, dorsal, lateral prostate, coagulating gland, bulbourethral gland) and the mammary gland of both Copenhagen and Wistar rats. Mono- and polyclonal antibodies directed against intermediate filament proteins (cytokeratin, desmin, vimentin) and the contractile proteins (α-actin, muscle type specific myosin, tropomyosin) were used along with phalloidin decoration of F-actin. As in the human prostate, none of the rat prostate lobes in either strain did contain basal cells expressing cytokeratin along with α-actin, myosin and tropomyosin. Cells representing fully differentiated myoepithelial cells, however, were present as anticipated in the mammary gland, the bulbourethral gland and the H-tumor line of the Dunning tumor. This finding is difficult to reconcile with the contention of a prostatic origin of the H-Dunning tumor. Further studies are required to classify the epithelial parental tissue in order to define the true origin of the H-Dunning tumor and the tumor lines derived thereof.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266961
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  • 9
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of villin in the normal human pancreas and in chronic pancreatitis (1991)
    Springer
    Histochemistry and cell biology 95 (1991), S. 383-390 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Human pancreatic tissue was investigated by immunohistochemistry using a polyclonal antibody against the actin binding protein villin, which participates in the formation of actin filament bundles in the microvilli. In cells of the different parts of the pancreatic duct system as well as in the acinar cells villin immunoreactivity was located mainly at the apical cell surface. This was confirmed by the ultrastructural demonstration of microvilli on the surface of duct and acinar cells, which exhibited the typical actin bundles. In chronic pancreatitis the staining for villin in duct-like structures of degenerative pancreatic tissue was irregular or even absent. This correlated with the electron microscopic observation of duct-like structures known as tubular complexes composed of cells devoid of microvilli at the apical cell surface. At the light microscopical level degenerative structures without lumen and of unknown origin showed a strong staining for villin at their basal cell surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266966
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  • 10
    Electronic Resource
    Electronic Resource
    The molecular basis of contractility Part I (1974)
    Springer
    Basic research in cardiology 69 (1974), S. 88-104 
    Details
    ISSN: 1435-1803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Muscular contraction stems from a sliding movement of thick and thin filaments relative to each other. These filaments run parallel to the direction of the muscle fibre. The thin filaments, which issue from the Z-membrane into the sarcomere, consist of 60% actin, and 40% regulator proteins (tropomyosin and troponin). The thick filament consists almost entirely of myosin. The two types of filaments form on overlapping region, the length of which depends on the degree of shortening of the muscle. The mechanical work which is produced by the muscle during shortening is produced by the so-called myosin cross-bridges, which appear to attach to actin, change their angle, and then detach in a cyclical “rowing-boat” manner. During such a cycle, ATP is split to ADP and inorganic phosphate, and therefore the production of mechanical work is based on the interaction of actin and myosin at the expense of chemical energy, which is supplied in the form of ATP. In the last 20 years, the structure of the involved proteins and their aggregation and ordering in the filaments has been widely investigated. Similarly, the rough nature of the electromechanical coupling process has been explained. i. e. the sequence of events which is necessary to activate a resting muscle into contraction via a neural impulse. At the present time, much interest is concentrated on the kinetic analysis of ATP hydrolysis and the modification of certain reaction steps by actin. From experiments of this sort, a more detailed and propound understanding of the nature of the mechanochemical energy coupling mechanism can be expected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01910790
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