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  • 1
    Electronic Resource
    Electronic Resource
    Degradation of polychlorinated biphenyls by hairy root culture of Solanum nigrum (1997)
    Springer
    Biotechnology letters 19 (1997), S. 787-790 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Polychlorinated biphenyls (PCBs) present in the commercial mixture Delor 103 were transformed by hairy root culture of Solanum nigrum. Plant growth regulators kinetin, 2,4-dichlorophenoxy-acetic acid, benzylaminopurin and/or naph-thaleneacetic acid, influenced the cells' growth and transformation of PCBs in a different manner. The cells were able to transform PCBs even if they ceased growing. Young inoculum (16 days) had a 20% lower PCB conversionthan did older inocula (37, 68 days) while biomass increase was much higher using young inoculum. With increasing size of inoculum, transformation of PCBs was stimulated. After 30 days of incubation the average amount of residual PCBs was 40% of the controls at initial PCB concentration of 100 ppm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018348511978
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Metabolism of polychlorinated biphenyls by Solanum nigrum hairy root clone SNC-9O and analysis of transformation products (2000)
    Springer
    Plant and soil 225 (2000), S. 109-115 
    Details
    ISSN: 1573-5036
    Keywords: environment ; hairy root ; phytoremediation ; plant cells ; polychlorinated biphenyls ; Solanum nigrum ; transformation products
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The study investigates aspects of PCB metabolism by a hairy root culture of Solanum nigrum L. (clone SNC-9O) in vitro. Standard conditions were established for efficient, up to 72% PCB conversion (22 individual PCB congeners examined in commercial mixture Delor 103, 5 g fresh biomass in 100 ml media shaken with 5 mg PCB for 14 days). The conversion products formed from three monochlorobiphenyls were monohydroxychlorobiphenyls and dihydroxychlorobiphenyls, while six dichlorobiphenyls yielded different monohydroxydichlorobiphenyls. Efficiency of the transformation of individual PCB congeners was evaluated together with phytotoxic effect on the clone SNC-9O. Major metabolites of monochlorobiphenyls analysed after extraction from biomass were hydroxylated at the position 4, and 4′, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026551728196
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A chemoenzymatic synthesis of the O-glycosides (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 10 (1998), S. 676-681 
    Details
    ISSN: 0899-0042
    Keywords: β-glucosidase from almond ; reverse hydrolysis ; transglucosylation ; enantioselectivity ; enantiomeric excess ; absolute configuration ; alkyl β-D-glucopyranoside ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Almond β-glucosidase was used to catalyze the synthesis of alkyl β-d-glucopyranosides 1b-3b starting from either d-glucose (4) or phenyl-β-d-glucopyranoside (5) and the racemic alcohols 1a-3a. The enzymic reactions were provided in different acetonitrile/water mixtures [9:1 (v/v) for the reverse hydrolysis, and 1:9 (v/v) for the transglycosylation]. Both enzymic reactions, that is, the reverse hydrolysis and the transglucosylation, are enantioselective processes. The enantiomeric purity of products 1b-2b of the enzymic reactions varied between 75 and 86% ee, the values of which were based on the analysis of the aglyconic parts (1c-2c) of the alkyl β-d-glucopyranoside molecules (1b-2b). Chirality 10:676-681, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 4
    Electronic Resource
    Electronic Resource
    Alkane assimilation ability of Pseudomonas C12B originally isolated for degradation of alkyl sulfate surfactants (1995)
    Springer
    Biotechnology letters 17 (1995), S. 765-770 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Pseudomonas C12B (NCIMB 11753) is able to utilize a broad range of alkyl sulfates. The growth on n-alkanes of different chain lenght (C6–C16) was tested. Pseudomonas C12B assimilated hydrocarbons from C9–C16. Growth rate on n-decane (1%) that was chosen as the typical sole source of carbon and energy depended on oxygen supply. The addition of surfactants (Triton X-100 and Tween-80) in a nontoxic concentrations resulted in increased biomass yield. Under optimal growth conditions Pseudomonas C12B exhibited the maximal growth rate and yield with C11 as the sole carbon source.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00130366
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    Paper (German National Licenses)
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