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  • 1
    Electronic Resource
    Electronic Resource
    A new approach to enhance growth and nodulation of Acacia mangium through aeroponic culture (1997)
    Springer
    Biology and fertility of soils 25 (1997), S. 7-12 
    Details
    ISSN: 1432-0789
    Keywords: Key words Aeroponic culture ; N2-fixation ; Acacia mangium ; Bradyrhizobium spp. ; Hypernodulation ; Tree saplings ; Imperata cylindrica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This work was designed to determine whether a plant culture method on non-solid media could be used as an alternative for inoculation of Acacia mangium with selected strains of Bradyrhizobium spp. A. mangium seedlings were grown and inoculated with Bradyrhizobium strain Aust13c and strain Tel2 in hydroponics, aeroponics and sand. Aeroponics was found to be the best system of the three, allowing the production of tree saplings 1 m in height after only 4 months in culture. Moreover, compared to plants grown in liquid or sand media, aeroponically grown saplings inoculated with Bradyrhizobium spp. developed a very high number of small nodules distributed all along the root system, resulting in an increase in nitrogen and chlorophyll content in plant tissues. We propose aeroponics as an alternative method to classical soil inoculation procedures for the production of hypernodulated legume tree saplings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050272
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterisation of Nocardioides sp. SP12, an atrazine-degrading bacterial strain possessing the gene trzN from bulk- and maize rhizosphere soil (2003)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 221 (2003), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: We report the characterisation of Nocardioides sp. SP12, an atrazine-degrading bacteria isolated from atrazine-treated bulk- and maize rhizosphere soil. Based on 16S rDNA alignment, strain SP12 showed close phylogenic relationships with Nocardioides sp. C157 and Nocardioides simplex. Internal transcribed spacer (ITS) sequences of strain SP12 were longer than those of other Nocardioides sp. and present Ala- and Ile-tRNA unlike Actinomycetales. Nocardioides sp. SP12 presents a novel atrazine catabolic pathway combining trzN with atzB and atzC. Atrazine biodegradation ends in a metabolite that co-eluted in HPLC with cyanuric acid. This metabolite shows an absorption spectrum identical to that of cyanuric acid with a maximal absorption at 214.6 nm. The mass of the atrazine metabolite is in concordance with that of cyanuric acid according to mass spectrometry analysis. Quantitative PCR revealed that the ITS sequence of Nocardioides sp. SP12 was at a lower number than the one of trzN in atrazine-treated soil samples. It suggests that trzN could also be present in other atrazine degrading bacteria. The numbers of trzN and ITS sequences of Nocardioides sp. SP12 were higher in the maize rhizosphere than in bulk soil.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0378-1097(03)00168-X
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Differential display analysis of RNA accumulation in arbuscular mycorrhiza of pea and isolation of a novel symbiosis-regulated plant gene (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 37-44 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsGlomus mosseae ; Pisum sativum L. ; Transmembrane protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differential RNA display was used to analyze gene expression during the early steps of mycorrhiza development on Pisum sativum following inoculation with Glomus mosseae. Seven out of 118 differentially displayed cDNA fragments were subcloned and sequenced. One fragment corresponded to part of the fungal 25S ribosomal RNA gene and a second one showed similarity to a human Alu element. The others were derived from plant genes of unknown function. One of the fragments was used for the isolation of a full-length cDNA clone. It corresponded to a single-copy gene (psam1) which is induced during early symbiotic interactions, and codes for a putative transmembrane protein. Northern and RNA dot blot analyses revealed enhanced accumulation of psam1 RNA after inoculation with G. mosseae of wild-type pea and an isogenic mutant deficient for nodule development (Nod−, Myc+).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050543
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    Paper (German National Licenses)
    Fulltext
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