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  • 1
    Electronic Resource
    Electronic Resource
    A new approach to enhance growth and nodulation of Acacia mangium through aeroponic culture (1997)
    Springer
    Biology and fertility of soils 25 (1997), S. 7-12 
    Details
    ISSN: 1432-0789
    Keywords: Key words Aeroponic culture ; N2-fixation ; Acacia mangium ; Bradyrhizobium spp. ; Hypernodulation ; Tree saplings ; Imperata cylindrica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This work was designed to determine whether a plant culture method on non-solid media could be used as an alternative for inoculation of Acacia mangium with selected strains of Bradyrhizobium spp. A. mangium seedlings were grown and inoculated with Bradyrhizobium strain Aust13c and strain Tel2 in hydroponics, aeroponics and sand. Aeroponics was found to be the best system of the three, allowing the production of tree saplings 1 m in height after only 4 months in culture. Moreover, compared to plants grown in liquid or sand media, aeroponically grown saplings inoculated with Bradyrhizobium spp. developed a very high number of small nodules distributed all along the root system, resulting in an increase in nitrogen and chlorophyll content in plant tissues. We propose aeroponics as an alternative method to classical soil inoculation procedures for the production of hypernodulated legume tree saplings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003740050272
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    DNA fingerprinting of Peronospora parasitica, a biotrophic fungal pathogen of crucifers (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 490-496 
    Details
    ISSN: 1432-2242
    Keywords: Peronospora parasitica ; Non-culturable pathogenic fungus ; DNA fingerprinting ; RAPDs ; Genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fungus Peronospora parasitica (Pers. ex Fr.) Fr. is an obligate biotroph infecting a wide range of host species in the family Cruciferae. Isolates from different hosts are morphologically similar, and pathotypes are usually distinguished on the basis of host range. Random Amplified Polymorphic DNA (RAPD) fingerprints were generated from a range of P. parasitica isolates from different Brassica species. Reaction conditions, in particular DNA template, primer and Mg2+ concentrations, were optimized to ensure that amplifications were reproducible. Possible artefacts arising through host plant DNA were assessed by including such DNA in control reactions. Confirmation that diagnostic RAPD bands were generated from fungal DNA was also obtained by Southern hybridization of a RAPD band to genomic fungal DNA. By screening 20 decamer primers, 2 were found to detect sufficient genetic variation to allow complete differentiation between pathotypes. These results illustrate the potential value of RAPDs for detecting polymorphisms between isolates of a non-culturable plant pathogenic fungus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223666
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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