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  • 1
    Electronic Resource
    Electronic Resource
    Transformation of haploid Datura innoxia protoplasts and analysis of the plasmid integration pattern in regenerated transgenic plants (1993)
    Springer
    Plant cell reports 12 (1993), S. 390-394 
    Details
    ISSN: 1432-203X
    Keywords: Datura ; haploids ; direct gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We developed a highly efficient transformation protocol for the PEG-mediated direct transfer of plasmid DNA into protoplasts of haploid Datura innoxia. Vectors harbouring a neomycin phosphotransferase II gene or a hygromycin B phosphotransferase gene under the control of different promoters were used in the transformation experiments. Various amounts of plasmid DNA were applied without any carrier DNA to show the direct influence of the plasmid DNA concentration on the transformation efficiency. Approximately 95% of the selected calli were regenerated to plants; 20% of them remained haploid. Total DNA of different transgenic plants was analysed with regard to the integration pattern of the plasmid DNA. Plants carrying only one or two copies of the vector DNA were observed as well as individuals with multi-copy integration (up to ten or more copies).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234698
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation of Vicia narbonensis via Agrobacterium-mediated gene transfer (1991)
    Springer
    Plant cell reports 9 (1991), S. 535-538 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Shoot tips and epicotyl-segments of Vicia narbonensis were co-cultivated with Agrobacterium tumefaciens strain C58C1 pGV 3850 HPT, carrying a plasmid coding for hygromycin-phosphotransferase. On callus-induction medium containing 60 mg/l hygromycin for selection, approximately 18% of the explants produced hygromycin-resistant callus. After transfer to regeneration-medium these calluses produced hygromycin-resistant and nopaline-positive somatic embryos which could be regenerated to plantlets. The integration of the T-DNA into the plant genome was confirmed by Southern analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232326
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Seed specific expression of the 2S albumin gene from Brazil nut (Bertholletia excelsa) in transgenicVicia narbonensis (1995)
    Springer
    Molecular breeding 1 (1995), S. 295-301 
    Details
    ISSN: 1572-9788
    Keywords: Brazil nut protein, grain legumes, leguminB4 promoter ; seed storage protein ; sulphur amino acid deficiency ; transgenic plants ; Vicia narbonensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to evaluate the possibility of enhancing the sulphur amino acids in grain legumes, we have transferred the gene for the methionine-rich 2S albumin from Brazil nut (Bertholletia excelsa) controlled by a seed-specific promoter intoVicia narbonensis. The coding region of the 2S albumin gene that has been completely synthesized was fused to the seed-specific leguminB4 promoter fromVicia faba. Transgenic lines ofV. narbonensis were generated viaAgrobacterium-mediated gene analysis of leaves, stems, roots and seeds of four R0 plants revealed that the expression of the foreign 2S albumin gene occurred in a seed-specific manner and that the foreign protein is present at levels ranging from 1% to 4.8% of total SDS-soluble seed protein. Since the current protocol for transformation ofV. narbonensis has not yet been published, a detailed description of the method is given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02277429
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Transcription and in vivo expression of aMicrocystis aeruginosa plasmid (1990)
    Springer
    Current microbiology 20 (1990), S. 365-368 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A restriction map of the plasmid pMA1 ofMicrocystis aeruginosa is presented. The total RNA of the plasmid-containing strain HUB 5-2-4 was isolated and hybridized with the resident plasmid, pMA1, of the strain HUB 5-2-4. A transcript of 1800 b was detected. The minicell-producing strain X 984 ofEscherichia coli was transformed with pMA1 cloned into pUC18. Two polypeptides sized 50 kD and 43 kD respectively were synthesized in a specific manner in transformed cells; this indicates that the cyanobacterial transcription and translation signals are recognized byE. coli.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02095861
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    Paper (German National Licenses)
    Fulltext
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