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  • 1
    Electronic Resource
    Electronic Resource
    Selecting and designing cell lines for improved physiological characteristics (1994)
    Springer
    Cytotechnology 15 (1994), S. 11-16 
    Details
    ISSN: 1573-0778
    Keywords: Chemostat ; cholesterol ; choline ; glutamine ; glutamine synthetase ; hybridoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract We have developed several approaches to create cell lines with improved characteristics in cell culture. In some cases it has been possible to isolate natural variants with useful properties. Cholesterol independent variants of the mouse NSO myeloma cell line were isolated by cloning in a selective medium. A glutamine independent variant of a hyridoma was isolated by continuous (chemostat) culture under glutamine limited conditions in the presence of glutamate. Choline independent cells were isolated from a choline limited chemostat. In an alternative approach to modifying cell behaviour, we have used recombinant DNA techniques to introduce the glutamine synthetase (GS) gene to a hybridoma. This resulted in glutamine independence and increased productivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00762375
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The mechanism of inhibition of ureogenesis by acidosis (1984)
    Springer
    Bioscience reports 4 (1984), S. 819-825 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In perfused rat liver a decrease of cytosol pH, determined with pH-sensitive microelectrodes7 from 7.2 to 6.85 is associated with a 50% fall in ureogenesis from ammonium chloride. In isolated rat hepatocytes the fall in ureogenesis due to acidosis is associated with decrease in the mitochondrial and cytosolic concentration of citrulline. Limitation of carbamoyl phosphate synthesis and thus citrulline supply could be responsible for the inhibition of ureogenesis observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01138163
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Hormonal modulation of hepatic plasma membrane lactate transport in cultured rat hepatocytes (1990)
    Springer
    Bioscience reports 10 (1990), S. 573-577 
    Details
    ISSN: 1573-4935
    Keywords: lactate transport ; gluconeogenesis ; cultured hepatocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hormonal modulation of hepatic plasma membrane lactate transport was studied in primary cultures of isolated hepatocytes from fed rats to examine the mechanism for the known enhancement of lactate transport in starvation and diabetes. Total cellular lactate entry was increased by 14% in the presence of dexamethasone; this was accounted for by an approximately 40% increase in the carrier-mediated component of entry with no effect on diffusion. A trend of similar magnitude was evident with glucagon. The effects of dexamethasone and glucagon on lactate transport constitute an additional potential mechanism for enhancement of gluconeogenesis by these hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116618
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    Paper (German National Licenses)
    Fulltext
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