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  • 1
    Electronic Resource
    Electronic Resource
    Permeability changes during cell fusion (1973)
    Springer
    The journal of membrane biology 14 (1973), S. 293-303 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary During Sendai virus-mediated fusion of mouse ascites cells, there is a loss of intracellular metabolites; at the same time accumulation from the medium is inhibited. This failure to maintain selective permeability does not occur at 0°C; it is unaffected by cytochalasin B, which inhibits fusion. It therefore represents a discrete, temperature-dependent event and may result from a weakening in membrane architecture necessary to achieve fusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868082
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  • 2
    Electronic Resource
    Electronic Resource
    Hexose transport in hybrids between malignant and normal cells (1982)
    [s.l.] : Nature Publishing Group
    Nature 296 (1982), S. 588-588 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] IT HAS been reported by White et a/.1 that a decreased Km for hexose transport correlates with malignancy in matched pairs of hybrids between malignant and normal cells. Hexose transport was measured by the uptake of 0.1-5 mM 2-deoxy[3H]glucose at 20 C; in these conditions, uptake was found to be ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/296588a0
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  • 3
    Electronic Resource
    Electronic Resource
    Microvilli and cell swelling (1976)
    [s.l.] : Nature Publishing Group
    Nature 262 (1976), S. 52-54 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Table 1 Binding of 131I-con A by Lettree cells Time of inc. Additions Control cells Swollen cells* (min) 131I-bound (c.p.m.)t 0 1 5,000 10,000 5 ? 68,000 72,000 10 . ? 107,000 105,000 5 a-MM 8,000 9,500 0 Cold con A 8,800 7,200 5 Cold con A 54,000 67,000 ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/262052a0
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  • 4
    Electronic Resource
    Electronic Resource
    Myxoviruses do not induce non-specific alterations in membrane permeability early on in infection (1983)
    Springer
    Archives of virology 77 (1983), S. 139-153 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The permeability characteristics of cells infected with myxoviruses have been studied by measuring the concentrative uptake of nutrients, the concentration of intracellular K+, and the maintenance of the Na+ gradient across the plasma membrane. Cells either show no change at all (Sendai virus-infected BHK cells and measles virus-infected Vero cells) or they show a decreased ability to concentrate nutrients, while intracellular K+ and the Na+ gradient remain unchanged (Sendai and influenza virus-infected L-1210 cells, measles virus-infected lymphocytes and mumps virus-infected L-41 cells). In no case, therefore, was a change observed that resembles the non-specific increase in membrane permeability induced by haemolytic paramyxoviruses (35, 42) or the non-specific membrane leakiness postulated to take place in infected cells (8, 9). A preliminary account of some of these findings has been presented (39).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309263
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  • 5
    Electronic Resource
    Electronic Resource
    Permeability changes resulting from virus-cell fusion: temperature-dependence of the contributing processes (1985)
    Springer
    Molecular and cellular biochemistry 66 (1985), S. 163-173 
    Details
    ISSN: 1573-4919
    Keywords: membrane fusion ; permeability change ; Sendai virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary A new assay for membrane fusion, using the fluorescent probe pyrene-sulphonyl-phosphatidyl ethanolamine, has been developed. Fusion between the envelope of Sendai virus and human erythrocytes or Lettre cells has a Q10 of ∼4 at 37° C, increasing to ∼7 at 7 ° C; there is no lag to onset of fusion. Viral neuraminidase has a Q10 of 2.3 between 37° C and 4° C. Its action limits the extent of fusion by causing the elution of virus; this effect is particularly marked at low temperature because of the difference in Q10 for fusion and neuraminidase. The temperature-dependence of the initiation of permeability changes following the removal of inhibitory amounts of Ca2+ is ∼2; thus membrane fusion is the principal temperature-sensitive step during the permeabilization of cells by Sendai virus. A recovery process, by which cells become insensitive to the removal of Ca2+ and which therefore limits the extent of permeabilization, has a Q10 of 7.4 between 37° C and 21° C. It is concluded that the lag to onset of permeability changes is not due to a lag in virus-cell membrane fusion, but to the gradual acquisition of a threshold level of membrane damage; the extent of permeabilization depends on the rate of fusion relative to the rates of neuraminidase and recovery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220784
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  • 6
    Electronic Resource
    Electronic Resource
    Virally induced alterations in cellular permeability: A basis of cellular and physiological damage? (1981)
    Springer
    Bioscience reports 1 (1981), S. 431-448 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Virally induced permeability changes occur when haemolytic paramyxoviruses are added to cells; similar (though not identical) changes take place during infection of ceils with viruses from several families (including paramyxoviruses). These changes occur in intact) viable cells) and precede subsequent cytopathic effects) to which they are likely to contribute. There is accumulating evidence to suggest that virally induced permeability changes may also underlie the physiological and clinical consequences of viral infection in certain situations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01121577
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  • 7
    Electronic Resource
    Electronic Resource
    Membrane changes during viral infection (1982)
    Springer
    Bioscience reports 2 (1982), S. 609-612 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314224
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  • 8
    Electronic Resource
    Electronic Resource
    A novel method for measuring intracellular pH and potassium concentration (1983)
    Springer
    Bioscience reports 3 (1983), S. 631-642 
    Details
    ISSN: 1573-4935
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The concentration of Na+and K+ and the pH in the cytoplasm of Lettré cells was measured by monitoring the net flux of H+, Na+, or K+ across the plasma membrane which had been rendered permeable to these ions by the action of Sendal virus. Ion flux was measured directly by analysis of cell composition, or indirectly by observing the change in membrane potential of cells treated with a specific ionophore. Cytoplasmic concentrations of cations were obtained by establishing the concentration of the cation in the medium at which addition of Sendai virus causes no change in cytoplasmic cation content. The value of Lettré-cell pH was confirmed by direct measurement employing 3tp nuclear magnetic resonance, and the values of Na+ and K+ concentration were confirmed by analysis of cell cation and water content. Lettré cells suspended at 32°C in Hepes-buffered saline at pH 7.3 maintain a cytosolic pH of 7.0 and contain 30 mM Na+ and 80 mM K+.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01172873
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  • 9
    Electronic Resource
    Electronic Resource
    Effect of Ca2+-antagonists on virally-induced cell-permeability changes (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 2 (1984), S. 249-253 
    Details
    ISSN: 0263-6484
    Keywords: Calcium ; Ca2+-antagonists ; calmodulin ; calmodulin inhibitors ; intracellular cation changes ; permeability changes ; virally-induced permeability changes ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Sendai virus-mediated permeability changes in cells are affected by extracellular Ca2+ or Mn2+ as follows: the lag period to onset of permeability changes is lengthened and the subsequent extent of leakage is reduced. Drugs that block Ca2+ action in excitable cells, such as verapamil and prenylamine, and drugs that inhibit the action of calmodulin, such as trifluoperazine and R24571, have an effect opposite to that of Ca2+: lag is shortened and extent of leakage is increased. The concentration at which either type of drug shows 50% of maximal effect is similar to the concentration at which 50% of binding by drug to calmodulin is achieved. It is concluded that calmodulin may be involved in protecting cells against virally-mediated membrane damage; alternatively the action of calmodulin-binding drugs may not be as specific as currently thought.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290020412
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