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  • 1
    Electronic Resource
    Electronic Resource
    Characteristics of 63.6° cylindrical energy analyzer used as charge exchange neutral particle analyzer (1999)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 70 (1999), S. 2661-2664 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A charge exchange neutral particle analyzer including a newly designed multichannel 63.6° cylindrical electrostatic energy analyzer was constructed. Since the energy analyzer can simultaneously adopt a small radius of cylindrical electrodes and a large width of an entrance slit, high incident current can be analyzed while the size of the analyzer remain compact. Characteristics of the analyzer were investigated using hydrogen beam with energies from about 0.5 to 5 keV. The experimental results are compared well with the numerical analysis. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1149825
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  • 2
    Electronic Resource
    Electronic Resource
    Fast two-dimensional potential measurement at the thermal barrier region of the tandem mirror Gamma 10 (1999)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 70 (1999), S. 4251-4259 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A gold neutral beam probe system was improved by adopting a multichannel ion detector and adding sweeping functions of beam energy, deflector voltage, and electrode voltage of the analyzer to the system to measure the fluctuations and time evolution of the two-dimensional space potentials with fast resolving time during one shot. Positions of the beam spot on the multichannel detector corresponding to the ionizing points of the beam in the plasma were simulated precisely as a function of the beam energy and the injection angle. A potential derivation formula was determined taking into consideration both the numerical and experimental results, and the reproducibility of the potential profile was checked. Two-dimensional potential profiles were measured in the optimization experiment of the microwave injection angles for formation of the plug potential. © 1999 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1150062
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  • 3
    Electronic Resource
    Electronic Resource
    Metabolic Activities of Rat Liver Preparations on Retinol, β-Carotene, and Lycopene (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 691 (1993), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1993.tb26198.x
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  • 4
    Electronic Resource
    Electronic Resource
    Differences within Mono- and Dizygotic Twin-Pairs in Spectrotypes and Clones of IgG2 Antibodies to Pneumococcal Polysaccharide Type 1 and C-Polysaccharide After Vaccination (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 40 (1994), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Spectrotypes and clones of antibodies against pneumococcal capsular polysaccharide (Ps) type 1 and C-polysaccharide (C-Ps) were determined before and after immunization with a polyvalent pneumococcal Ps vaccine in 84 mono- or dizygotic twins. The method used was a micromodification of a rapid isoelectric focusing-amnity (lEF-affinity) immunoblot technique in agarose permitting characterization of isotype, light chain and Gm type. After vaccination the anti-type 1 Ps + anti-C-Ps clones were different in 75% of the monozygotic and 79% of the dizygotic twins. The anti-type 1 Ps clones differed among 72% of the monozygotic and 85% of the dizygotic twins (P 〉 0.05). Each twin had from zero to three clones producing IgG2 antibodies against type 1 Ps, A total of six different clones could be distinguished among all the twins. Vaccination enhanced the already actively secreting B-cell clones in 56 twins and newly recruited clones in 11 of the 84 twins; six among the 48 mono- and five among the 36 dizygotic twins. These new clones differed among the twins. Spectrotypes varied between all twins within the pairs. The fact that all twins differed in spectrotype is due to post-translational microheterogenity of the antibodies, events which are thus not genetically determined. The observation that even mono zygotic twins possessed and responded with different clones within the pairs indicates that the V-region genes, which determine the final specificity of B cells, either differ from the original germ-line V region genes, e. g. owing to hypermutations or junctional diversity, or the rearranged germ-line genes occur accidentally although highly restricted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1994.tb03484.x
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  • 5
    Electronic Resource
    Electronic Resource
    Human IgG but not IgM Antibodies can Protect Mice from the Challenge with Live O6 Escherichia coli (2005)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 62 (2005), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We evaluated the ability of human anti-lipopolysaccharide (LPS) O6 immunoglobulin G (IgG) and IgM antibodies to protect mice challenged with Escherichia coli serotype O6:K2ac. Purified whole IgG, commercial gammaglobulin, whole IgM-effluent, pool of normal human serum (NHS), agammaglobulinaemic serum (test groups) or phosphate-buffered saline (control group) was injected into adult male 18 h before a challenge with viable O6 E. coli. The mortality rate was assessed over a period of 72 h. To determine the opsonic and phagocytic activity of the antibody isotypes, we incubated peritoneal macrophages from the control and test groups collected at different times after challenge with the live bacteria with acridine orange for fluorescent analysis. Tumour necrosis factor (TNF)-α and interleukin (IL)-6 were quantified in serum of both the test and control groups. All mice that received commercial gammaglobulin or NHS survived. Purified whole IgG (containing 1.1 mg/l of anti-LPS O6 IgG antibodies) protected 87.5% of the animals tested in this experiment, while whole IgM-enriched effluent with 1.5 mg/l of anti-LPS O6 IgM antibodies protected only 12.5%. The agamma serum showed no protective capacity compared with PBS (serving as control). The minimal concentration of anti-LPS O6 IgG antibodies able to protect 50% of animals was 0.137 mg/l of purified whole IgG. Whole IgM-enriched effluent showed no protective capacity independently of the concentration tested (0.048–17.0 mg/l of anti-LPS O6 IgM antibodies). Fluorescent analysis of peritoneal macrophages from animals pretreated with purified whole IgG showed no bacteria at 8 h after the challenge. By contrast, whole IgM effluent showed an increasing number of live bacteria at the same time. Mice that had received whole IgM effluent (1.5 mg/l of anti-LPS O6 IgM antibodies) before the challenge with LPS O6 presented 20.5 µg/l of IL-6 and 1.5 µg/l of TNF-α. Serum from animals pretreated with purified IgG did not present any detectable pro-inflammatory cytokine. Our findings suggest that IgG but not IgM antibodies protect animals from a challenge with E. coli O6 serotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.2005.01669.x
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  • 6
    Electronic Resource
    Electronic Resource
    Elevated Levels and Different Repertoire Profile of Colostral Anti-LPS Antibodies May Have a Significant Role in Compensating Newborn Immunity (2001)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 53 (2001), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A high prevalence of systemic infections caused by enterobacteria such as Escherichia coli is observed during the neonatal period. Lipopolysaccharide (LPS) is one of the major factors responsible for septic shock caused by these Gram-negative bacteria. We have recently demonstrated the presence of anti-LPS immunoglobulin (Ig)G antibodies in cord blood with a repertoire identical to that found in maternal serum. In the present study, we analyzed anti-LPS O111 antibody isotypes in maternal serum and colostrum from mothers and in cord serum from their respective full-term (n = 30) and preterm (n = 13) neonate infants. The main isotype found in serum samples from mothers of term infants was IgM (range between 28 and 54 mg/l), followed by IgA (1–2 mg/l) and IgG (2–3 mg/l). The range of IgG antibody concentrations in cord blood was between 2 and 3 mg/l, as a result of placental transfer. A novel observation in our study was that the LPS bands recognized by colostral antibodies were completely different from those recognized by IgG in serum. Colostral IgA antibodies recognized several bands not bound by serum IgG antibodies from the respective maternal serum, independently of the antibody quantity. In addition, we verified the pattern of LPS recognition by serum IgA and colostral IgA antibodies was identical, what suggested that the antibody isotype found in serum could probably be derived from differentiated IgA-positive cells which were homing to the mucosa through the mucosal homing mechanism. Identical pattern of recognition was obtained comparing the IgA and IgM isotypes in colostrum. Slight differences in the pattern of recognition were found between colostral and serum IgM antibodies. The fact that colostral antibodies recognize much more bands than serum antibodies may be important for the host to mount an effective immune response in the intestinal lumen, in order to prevent excessive absorption of LPS, reducing possible systemic effects caused by the molecule.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.2001.00921.x
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  • 7
    Electronic Resource
    Electronic Resource
    Beam emittance measurements of magnetron type negative ion source
    Amsterdam : Elsevier
    Nuclear Instruments and Methods 184 (1981), S. 583-585 
    Details
    ISSN: 0029-554X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0029-554X(81)90768-0
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of lard, palm and rapeseed oils life conservation in aged mice
    Amsterdam : Elsevier
    Mechanisms of Ageing and Development 60 (1991), S. 267-274 
    Details
    ISSN: 0047-6374
    Keywords: Brain ; Dietary fat and oils ; Docosahexaenoic acid ; Linolenic acid ; Liver ; Survival
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0047-6374(91)90039-3
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  • 9
    Electronic Resource
    Electronic Resource
    Antioxidant activity of 6-phosphatidyl-L-ascorbic acid
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 172 (1990), S. 385-389 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(90)90684-F
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  • 10
    Electronic Resource
    Electronic Resource
    Antioxidant activity of phosphatidyl-L-ascorbic acid
    Amsterdam : Elsevier
    Free Radical Biology and Medicine 9 (1990), S. 18 
    Details
    ISSN: 0891-5849
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0891-5849(90)90231-7
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