ISSN:
1573-739X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract In a joint effort, 4 existing Quality Control programmes organized in September 1978 a test on the determination of antiepileptic drugs and made an inventory of the various methods of analysis used. To approx. 700 laboratories 3 lyophilized calf s serum specimens, each of them spiked with 8Ae drugs, were distributed together with an extensive questionnaire. The number of laboratories reporting results, were: phenobarbital 601, phenytoin 624, carbamazepine 398, 10, 11- epoxide-Cbz 15, primidone 434, ethosuximide 302, valproic acid 167 and clonazepam 26; approx. 95% of them also completed the questionnaire. As to themethods per Q.C. programme,Emit was mainly used in USA,Glc in Europe,Hplc in USA (Rp systems) and in The Netherlands (sp systems),Ria in USA and Scandinavia. As to theresults per method,Emit, Glc andHplc produced similar results in therapeutic and sub-toxic concentrations ofPb, Dph andPri; in the sub-therapeutic range of these drugs,Glc andHplc gave better results thanEmit (which produced values 10–20% higher than the theoretical ones). InEsm andVal, Glc-methods are preferred while inCbz, Hplc is the method of choice, followed byEmit. InClona, severalGlc andHplc methods are still under investigation. Spectrophotometric determinations (mainly used inPb, Dph andCbz) proved to be not reliable. In tables, results per technique are subdivided into approx. 5 methods. This enables to distinguish betweene.g. Glc-derivatization with and without previous clean up or betweenEmit-automatic 〈 10Μl and 50Μl samples. Per drug, the various methods of assay are discussed; also, methods and results are given when drugs were determined simultaneously. Bovine serum (calfs serum) proved to be a suitable matrix in all methods used.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02293428
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