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  • 1
    Electronic Resource
    Electronic Resource
    Differential expression of glial fibrillary acidic protein in human glioma cell lines (1989)
    Springer
    Acta neuropathologica 78 (1989), S. 9-15 
    Details
    ISSN: 1432-0533
    Keywords: Glioma ; Cell lines ; Glial fibrillary acidic protein ; cDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have obtained a cDNA fragment to human glial fibrillary acidic protein (GFAP) by immunoscreening a λgt11 human brain cDNA library with antibody to bovine GFAP. The highly homologous nucleotide sequence of this clone with that of the mouse GFAP enabled the identification of this cDNA as one encoding GFAP. As this cDNA hybridized with a single major RNA species in Northern blots of RNA from human and mouse brain tissues and gave one or two bands in Southern blots of human genomic DNA, it was considered to be specific for GFAP. Using this cDNA as a probe we investigated the levels of GFAP expression in ten human glioma cell lines. A 3.5-kb GFAP mRNA was detected in five of the ten glioma cell lines, one of which was U-251 MG cell line and the other four were clones derived from the same tumor (CL1, 2, 3, and 4). There was a difference in the amount of GFAP mRNA among U-251 MG and the four clonal cell lines. Quantitative evaluation of this difference by RNA dot blot analysis revealed that the amount of GFAP mRNA expressed in CL3 was about 1/5 and in CL4 about 1/10 the amount expressed in U-251 MG, CL1, and CL2. Semiquantitative Western blot analysis showed that GFAP levels corresponded to the GFAP mRNA levels in these cell lines. By Southern blot analysis of genomic DNA the GFAP gene was similarly detected in all of these cell lines regardless of the level of GFAP expression. Thus, by using a cDNa to human GFAP we have demonstrated the presence of clonal cell lines from human glioma showing different levels of GFAP expression, which may provide a useful basis for further investigations on the regulation of GFAP gene expression in glial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687396
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  • 2
    Electronic Resource
    Electronic Resource
    Correlation of DNA ploidy and morphological features of human glioma cell cultures with the establishment of cell lines (1988)
    Springer
    Acta neuropathologica 76 (1988), S. 433-440 
    Details
    ISSN: 1432-0533
    Keywords: Glioma ; Tissue culture ; DNA ploidy ; Morphology ; Established cell lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eleven gliomas were serially cultivated and examined for DNA distribution by flow cytometry and simultaneously for morphological features by light microscopy at the various passage levels until passage 50 at most. Seven gliomas (four low-grade gliomas, three anaplastic gliomas) showed a similar DNA distribution pattern with a main diploid and small tetraploid peaks at various passages. In this group, only one culture formed a permanent cell line, whereas six cultures showed a limited growth ranging from 6 to 24 passages. In contrast, the other four gliomas (each an anaplastic glioma) showed a marked change of DNA distribution through passages and finally a single DNA aneuploid population prospered. Each of these four gliomas yielded established cell lines. Thus, it is suggested that the change of DNA ploidy and prosperity of DNA aneuploid populations in flow cytometry might be used as early and reliable indices for the later establishment of glioma-derived permanent cell lines. Since the changes of DNA distribution are frequently associated with the morphological changes, as seen in the latter group, careful tracing of morphological features is valuable in determining of the fate of cultures, especially in the absence of a flow cytometer. The correlation between the potential to become established cell lines and histology of the original gliomas is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686381
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  • 3
    Electronic Resource
    Electronic Resource
    Regional differences in bromodeoxyuridine uptake, expression of Ki-67 protein, and nucleolar organizer region counts in glioblastoma multiforme (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 586-593 
    Details
    ISSN: 1432-0533
    Keywords: Glioblastoma ; Proliferative potential ; Bromodeoxyuridine ; Ki-67 ; Nucleolar organizer region
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate intratumoral differences in indices of tumor cell proliferation, we measured the bromodeoxyuridine labeling index (BrdUrd LI), the Ki-67 protein proliferating cell indices (PCIs) determined by monoclonal antibody MIB 1 in microwave-processed paraffin sections (MIB 1 PCI) and in some eases by monoclonal antibody in frozen sections (Ki-67 PCI), and counts of argyrophilic nucleolar organizer regions (AgNORs) in 20 glioblastomas. In the most actively proliferating areas, MIB 1 and Ki-67 PCIs correlated well with the BrdUrd LI and with each other, while AgNOR counts correlated less strongly with these indices. In less active areas, the MIB 1 PCI and BrdUrd LI changed concomitantly from one area to another within a tumor except in areas of pseudopalisading with necrosis; in these areas the BrdUrd LI decreased significantly compared with neighboring tumor tissue, while the MIB 1 PCI did not. There was very little staining of gemistocytic nuclei with either anti-BrdUrd or MIB 1 monoclonal antibodies; this supports the concept that gemistocytes are mainly quiescent cells. AgNORs in all of the above-mentioned areas varied from tumor to tumor, which suggests that they may indicate some cellular activity other than proliferation. The close correlation between the BrdUrd LI and Ki-67 protein PCIs in corresponding regions of glioblastomas suggests that MIB 1 staining of microwave-processed paraffin sections can be used to evaluate the growth potential of individual glioblastomas and possibly of other gliomas as well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293319
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  • 4
    Electronic Resource
    Electronic Resource
    Regional differences in bromodeoxyuridine uptake, expression of Ki-67 protein, and nucleolar organizer region counts in glioblastoma multiforme (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 586-593 
    Details
    ISSN: 1432-0533
    Keywords: KeyWordsGlioblastoma ; Proliferative potential Bromodeoxyuridine ; Ki-67 ; Nucleolar organizer region
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate intratumoral differences in indices of tumor cell proliferation, we measured the bromodeoxyuridine labeling index (BrdUrd LI), the Ki-67 protein proliferating cell indices (PCIs) determined by monoclonal antibody MIB 1 in microwave-processed paraffin sections (MIB 1 PCI) and in some cases by monoclonal antibody in frozen sections (Ki-67 PCI), and counts of argyrophilic nucleolar organizer regions (AgNORs) in 20 glioblastomas. In the most actively proliferating areas, MIB 1 and Ki-67 PCIs correlated well with the BrdUrd LI and with each other, while AgNOR counts correlated less strongly with these indices. In less active areas, the MIB 1 PCI and BrdUrd LI changed concomitantly from one area to another within a tumor except in areas of pseudopalisading with necrosis; in these areas the BrdUrd LI decreased significantly compared with neighboring tumor tissue, while the MIB 1 PCI did not. There was very little staining of gemistocytic nuclei with either anti-BrdUrd or MIB 1 monoclonal antibodies; this supports the concept that gemistocytes are mainly quiescent cells. AgNORs in all of the above-mentioned areas varied from tumor to tumor, which suggests that they may indicate some cellular activity other than proliferation. The close correlation between the BrdUrd LI and Ki-67 protein PCIs in corresponding regions of glioblastomas suggests that MIB 1 staining of microwave-processed paraffin sections can be used to evaluate the growth potential of individual glioblastomas and possibly of other gliomas as well.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293319
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  • 5
    Electronic Resource
    Electronic Resource
    Proliferative Potential of Recurrent Intracranial Meningiomas as Evaluated by Labelling Indices of BUdR and Ki-67, and Tumour Doubling Time (1998)
    Springer
    Acta neurochirurgica 140 (1998), S. 26-32 
    Details
    ISSN: 0942-0940
    Keywords: Keywords: Recurrent meningioma; bromodeoxyuridine; Ki-67; tumour doubling time
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study was designed to provide the reciprocal relationship among labelling indices of 5-bromodeoxyuridine (BUdR LI), Ki-67 (Ki-LI), and tumour doubling time (Td) of recurrent meningiomas. In our series of 182 primary intracranial meningiomas, 46 cases recurred. The average of BUdR LI and Ki LI for nonrecurrent meningiomas were 0.77±0.13% and 4.71±1.96%, respectively. Recurrent meningiomas had significantly higher LIs at the first operation: BUdR LI was 3.77±1.22% and Ki LI was 14.78±3.17%. The recurrent ratio significantly increased with the degrees of each LI. And the linear regression analysis has demonstrated a significant correlation between BUdR and Ki LI. Td was calculated accurately by NIH, a computer software. Td showed a significant inverse correlation with each of the labelling indices. Consequently, BUdR, Ki LIs and Td of individual tumours correlate mutually well. Of the 46 recurrent cases, 4 received radiation after the operation. Td of the irradiated meningiomas tended to be longer than expected for their higher level of BUdR and Ki LIs before radiation therapy. Thus, it was shown that the radiation therapy delays the regrowth of meningiomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007010050053
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  • 6
    Electronic Resource
    Electronic Resource
    Matrix effective potential for electronic response in electron scattering with application to He at 30-400 eV impact energy
    Amsterdam : Elsevier
    Physics Letters A 76 (1980), S. 119-120 
    Details
    ISSN: 0375-9601
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0375-9601(80)90587-3
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  • 7
    Electronic Resource
    Electronic Resource
    New version of program for calculating differential and integral cross sections for quantum mechanical scattering problems from reactance or transition matrices
    Amsterdam : Elsevier
    Computer Physics Communications 21 (1980), S. 97-108 
    Details
    ISSN: 0010-4655
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Computer Science , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0010-4655(80)90079-X
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  • 8
    Electronic Resource
    Electronic Resource
    New version of program for calculating differential and integral cross sections for quantum mechanical scattering problems from reactance or transition matrices
    Amsterdam : Elsevier
    Computer Physics Communications 35 (1984), S. C-653 
    Details
    ISSN: 0010-4655
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Computer Science , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0010-4655(84)82813-1
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Quantum-mechanical calculations of the collinear rearrangement reaction He + H^+"2 -〉 HeH^+ + H at energies up to the dissociation threshold
    Amsterdam : Elsevier
    Chemical Physics Letters 226 (1994), S. 227-234 
    Details
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-2614(94)00737-3
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  • 10
    Electronic Resource
    Electronic Resource
    Quantum mechanical close coupling calculations of collision-induced dissociation in He+H"2. A discretized continuum model
    Amsterdam : Elsevier
    Chemical Physics Letters 216 (1993), S. 613-618 
    Details
    ISSN: 0009-2614
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0009-2614(93)90151-P
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