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  • 1
    Electronic Resource
    Electronic Resource
    Development, differentiation and derivation of the endocrine polypeptide cells of the mouse pancreas (1973)
    Springer
    Diabetologia 9 (1973), S. 120-129 
    Details
    ISSN: 1432-0428
    Keywords: Pancreatic islets ; endocrine (APUD) cells ; embryology ; neural crest ; immunofluorescence ; ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Studies on the developing mouse pancreas indicate that neuroectodermal cells from the neural crest, identifiable by their APUD-FIF characteristics, colonize the foregut at around the 10th day. Carried into the pancreatic anlagen, their primitive pleomorphic granules are progressively replaced by spherical granules which are ultimately (around 16 days) identifiable as of A, B or D type. — Insulin and glucagon are first demonstrable, by immunofluuorescence, at the 14th day, at which time zymogen granules are detectable by electron microscopy. — It is postulated that the neuroectodermal cell of the neural crest may be the precursor of some or all of the three known endocrine cells of the pancreatic islets. In the case of the A and D cells present evidence is considered sufficiently strong to make this a tenable hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01230691
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  • 2
    Electronic Resource
    Electronic Resource
    Periventricular astrocytosis and acute necrotizing encephalitis (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 90-93 
    Details
    ISSN: 1432-0533
    Keywords: Encephalitis, Acute Necrotizing ; Astrocytosis (Proliferation of Astrocytes) ; Subependymal Cells ; Choroid Plexus ; Enzyme Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine Serie von Ratten mit akuter nekrotisierender Encephalitis wurde untersucht. Das histopathologische Bild war bei allen Tieren gleich. Die periventrikulären Abschnitte, in denen kein morphologischer Hinweis auf Nekrose oder Infektion bestand, zeigten eine floride astrocytäre Reaktion. Die periventrikuläre Astrocytose war mit histologischen Routinemethoden nicht nachweisbar, ließ sich aber enzymhistochemisch gut darstellen. Es wird vermutet, daß diese Astrocyten aus dem Ependym stammen, wobei eine mögliche celluläre Beteiligung seitens des Plexus chorioideus angenommen wird.
    Notes: Summary A group of rats presented with acute necrotizing encephalitis. The histopathological picture was the same in all the animals yet the periventricular regions where no morphologically appreciable signs of necrosis or infection could be found, presented with a florid astrocytic response. This periventricular astrocytosis was undetected with standard morphological methods but well-demonstrated with histoenzymatic techniques. It is presumed that these astrocytes originated in the ependyma with a possible cellular contribution from the choroid plexus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00685313
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The solitary active cells histochemical demonstration of damage-resistant nerve cells with a TPN-diaphorase reaction (1964)
    Springer
    Acta neuropathologica 3 (1964), S. 238-249 
    Details
    ISSN: 1432-0533
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00684399
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  • 4
    Electronic Resource
    Electronic Resource
    A pericapillary cell in the central nervous system of the human embryo and adult (1965)
    Springer
    Acta neuropathologica 4 (1965), S. 442-445 
    Details
    ISSN: 1432-0533
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eine Zellart wird beschrieben, welche in engem Kontakt mit den Capillaren des menschlichen Zentralnervensystems steht und im Gehirn schon in der siebenten Embryonalwoche nachweisbar ist.
    Notes: Summary A cell is described, which appears to be in close attachment to the capillaries of the human central nervous system; and which is seen in the brain as early as the seventh week of embryonic life.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690681
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  • 5
    Electronic Resource
    Electronic Resource
    Anti-gastrin immunofluorescence in the skin ofHyla crepitans and the cytochemistry of the cells involved (1970)
    Springer
    Cellular and molecular life sciences 26 (1970), S. 288-289 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Mit indirekter Immunofluoreszenztechnik wird demonstriert, dass Anti-Gastrinserum (IgG-Fraktion) mit Hautzellen und mit zur Haut gehörenden Drüsen vonHyla crepitans reagiert. Diese, wahrscheinlich Caerulein enthaltenden Zellen, besitzen endokrine Eigenschaften.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01900100
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  • 6
    Electronic Resource
    Electronic Resource
    Zur cytochemischen Lokalisation einiger Glykosidasen und anderer saurer Hydrolasen im Resorptionsepithel des menschlichen Dünndarms (1967)
    Springer
    Journal of molecular medicine 45 (1967), S. 383-387 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Studying the site of hydrolytic enzymes with acid ph-optima in human intestinal absorptive cells the glycosidasesβ-glucosaminidase,β-glucoronidase,α-glucosidase andβ-galactosidase as well as the hydrolases non-specific acid phosphatase, E 600 resistent esterase and arylsulfatase have been investigated using histochemical techniques. Non-specific acid phosphatase activity has been localized additionally by electron cytochemical methods. Reaction product of the above activities is confined to a number of granules lined up beneath the brush border except forα-glucosidase andβ-galactosidase which are not demonstrable on a cytochemical level. Arylsulfatase activity is missing in human intestinal absorptive cells with the technique used but has been shown in corresponding sites of the small intestinal absorptive cell ofPatella vulgata. In agreement with the electron cytochemical demonstration of non-specific acid phosphatase activity and also with earlier biochemical data the granular activity of the various enzymes can be related to lysosomes. The meaning of these findings in relation to absorption is discussed.
    Notes: Zusammenfassung Ausgehend von der Frage nach dem Ort der Substratspaltung der sauren hydrolytischen Enzyme in der Resorptionszelle des menschlichen Dünndarms wurden die Glykosidasenβ-Glucuronidase,β-Glucosaminidase,α-Glucosidase undβ-Galaktosidase sowie die Hydrolasen unspezifische saure Phosphatase, E 600 resistente Esterase und Arylsulfatase mit enzymhistochemischen Methoden untersucht. Die saure Phosphatase wurde darüber hinaus mit elektroncytochemischer Technik lokalisiert. Das Reaktionsprodukt dieser Aktivitäten ließ sich mit Ausnahme der Disaccharidasenα-Glucosidase undβ-Galaktosidase lokalisieren. Es war in feinen Granula unmittelbar unterhalb des aktivitätsfreien Bürstensaumes angeordnet. Eine Arylsulfatase-Aktivität konnte am Resorptionsepithel des Menschen nicht nachgewiesen werden. Die genannten Disaccharidasen zeigten eine diffuse Verteilung des Reaktionsproduktes mit einem Intensitätsmaximum im apikalen Zellanteil. In Übereinstimmung mit dem elektroncytochemischen Bild und mit früheren biochemischen Befunden wird die granuläre Aktivität auf die Lysosomen bezogen. Die Bedeutung der Befunde in bezug auf die Resorption wird diskutiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01738748
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  • 7
    Electronic Resource
    Electronic Resource
    A Direct Histochemical Method for the Demonstration of Sialic Acid (1963)
    [s.l.] : Nature Publishing Group
    Nature 198 (1963), S. 1273-1275 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] A DIRECT histochemical method for demonstrating the presence of sialic acid using Bial's reagent was described by Diezel1. This method gives positive results in conventionally fixed tissues only when the histological dye orcein is substituted, as it sometimes inadvertently is, for the required ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1981273a0
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  • 8
    Electronic Resource
    Electronic Resource
    Cytochemical Demonstration of Ubiquinones in Animal Tissues (1963)
    [s.l.] : Nature Publishing Group
    Nature 199 (1963), S. 1063-1066 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SUCCINATE dehydrogenase cannot be demonstrated histochemically in material embedded in paraffin wax1. Nor can it be demonstrated in freeze-dried blocks, or in air-dried cryostat sections, if these have been incubated for brief periods in low melting point waxes. While studying this enzyme ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1991063a0
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  • 9
    Electronic Resource
    Electronic Resource
    Leucine Aminopeptidase in Rat Parathyroid and its Relation to Parathyroid Hormone Production (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 1532-1533 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The glands were removed from the animals with a thin rim of adjacent thyroid tissue and embedded in liver to prevent distortion during sectioning. Fresh frozen 8-jx sections were cut on a remotely controlled cold microtome (cryostat) and incubated in a medium containing L-leucyl-p-naphthylamide ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/1811532a0
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  • 10
    Electronic Resource
    Electronic Resource
    Cytochemical Localization of Succinic Dehydrogenase in Mitochondria from Periplaneta americana (1958)
    [s.l.] : Nature Publishing Group
    Nature 181 (1958), S. 702-703 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Histochemical methods for showing this enzyme system depend on the acceptance of hydrogen by water-soluble tetrazolium salts and their reduction to coloured, insoluble, formazan dyes. N R H O N N E H Tetrazolium i H N "NT Formazan This reaction is not reversible in biological ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/181702a0
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