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  • 1
    Electronic Resource
    Electronic Resource
    Improvement of the Adhesion and Proliferation of Human Osteoblastic-Like Cells with Biomimetic Nanoapatite Coatings (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 385-388 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Biomimetic nanoapatite coatings was developed by functionally modified methods with acombination of topographic, chemical and biomimetic treatments on the surface of titanium (Ti)substrate. The biological behavior and bioactivity of functionally modified SLA implants withchemical and biomimetic treatments (SCB-treated Ti) using body like solution were investigated tocompare with untreated Ti and SLA Ti plates as controls. The cell attachment, proliferation,alkaline phosphotatse (AKP) activity, cell morphology and differentiation were evaluated by usingMTT, RT-PCR, scanning electron microscopy (SEM) and confocal laser-scanning microscope(CLSM) analysis system. The results showed that the cell adhesion and proliferation was enhancedon functionalized titanium surface with nano-scale apatite compared to the controls. SEMmicrographs also revealed that the osteoblast-like cells spreadly grew along the surface. Cellmorphology and differentiation could be further observed distinctly by CLSM graphs. Moreover,mRNA expression of alkaline phosphotatse in nucleus on the SCB-treated Ti increased obviouslyon the third day compared with the controls. The in vitro results demonstrated the remarkableimprovement on cell adhesion and proliferation of the biomimetic nanoapatite on SCB-treated Ti,which could be used for orthopaedic/dental implants
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/52/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.385.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Biological Efficacy of Modified Structure of Polymer Surfaces for Bone Substitute (2007)
    s.l. ; Stafa-Zurich, Switzerland
    Key engineering materials Vol. 330-332 (Feb. 2007), p. 707-710 
    Details
    ISSN: 1013-9826
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Hydroxyapatite (HA) coatings were introduced onto Poly L-lactic Acid (PLLA) polymerin a controlled manner by immobilized urease method with a shortened precipitation time.Osteoblastic-like cellular responses to the composite were examined in terms of cell proliferation,differentiation and cell morphology, as well as the expression of bone-associated genes. The cellsexhibited higher cellular proliferation at 2 and 4 days on the HA/PLLA composite compared toPLLA scaffold, while no significant difference was observed later at 6 days. The alkalinephosphatase (ALP) activity by cells at 7 days was statistically higher on HA/PLLA scaffold than onPLLA. Moreover, the gene expression of ALP and osteocalcin (OC) was up regulated on HA/PLLAcomposite by RT-PCR analysis. The preliminary study suggested that the use of the controlledmodification of hydroxyapatite coating on PLLA scaffold to produce HA/PLLA composite mightenhance cellular activity, indicating the potential use for bone substitute in tissue engineering
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/53/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.330-332.707.pdf
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  • 3
    Electronic Resource
    Electronic Resource
    Recombination and clonal groupings within Helicobacter pylori from different geographical regions[Authors' n] (1999)
    Oxford BSL : Blackwell Science Ltd
    Molecular microbiology 32 (1999), S. 0 
    Details
    ISSN: 1365-2958
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology , Medicine
    Notes: A collection of 20 strains of Helicobacter pylori from several regions of the world was studied to better understand the population genetic structure and diversity of this species. Sequences of fragments from seven housekeeping genes (atpA, efp, mutY, ppa, trpC, ureI, yphC ) and two virulence-associated genes (cagA, vacA) showed high levels of synonymous sequence variation (mean percentage Ks of 10–27%) and lower levels of non-synonymous variation (mean percentage Ka of 0.2–5.6%). Cluster analysis of pairwise differences between alleles revealed the existence of two weakly clonal groupings, which included half of the strains investigated. All six strains isolated from Japanese and coastal Chinese were assigned to the ‘Asian’ clonal grouping, probably reflecting descent from a distinct common ancestor. The clonal groupings were not totally uniform; recombination, as measured by the homoplasy test and compatibility matrices, was extremely common within all genes tested, except cagA. The fact that clonal descent could still be discerned despite such frequent recombination possibly reflects founder effects and geographical separation and/or selection for particular alleles of these genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01382.x
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