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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Chromatography A 445 (1988), S. 285-289 
    ISSN: 0021-9673
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biotechnology techniques 9 (1995), S. 705-708 
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A simple system for the aerobic microbial degradation of volatile substances without stripping losses is described. The air circulates in a closed system, which differs from others by the way in which the O2 consumed is supplemented. The system does not require any measuring or control equipment. We have used the proposed device for the degradation of diesel fuel and kerosene in soil columns.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 16 (1996), S. 237-244 
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The remediation of polluted soils by microbial communities is a complex process, characterized by many competitive and cooperative mutual relations between the individual organisms. For the overall characteristic of such processes, top-down systems analyses promise a better description for prognostic aims than bottom-up approaches.This is demonstrated using the EVOLON model to fit the cumulative O2 consumption data of a microbial ecosystem metabolizing diesel fuel in polluted soil. The EVOLON is a generic top-down model providing close approximation to the experimental data.The advantage of using this model instead of other similar models is demonstrated by a comparison of the deviations between model values and experimental data (residuals).
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 356 (1996), S. 378-384 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The residual fractions remaining after microbial degradation of diesel fuel, different deparaffinized raffinates and extracts from long-term contaminated soils were analyzed by liquid chromatography, gas chromatography, infrared spectrometry and mass spectrometry. The quantity of saturated hydrocarbons decreased after the microbial treatment, whereas the portion of polar compounds increased. The total content of aromatics changed only insignificantly. n-Paraffins 〈 C26 were found to be no longer present in mineral oils degraded to exhaustion. Infrared spectrometry revealed oxygen compounds in the residues, mainly ketones, fatty acids and esters. Elementary analysis confirms the presence of nitrogen, oxygen and sulphur compounds in the degraded products. The gas chromatograms of high boiling oils, as well as of residues and extracts, consist mainly of a large base “envelope” (about 95% of the total area); thus gc/ms coupling reaches the limits of its applicability. However, mass spectrometry with direct inlet gives valuable information regarding hydrocarbon type analysis. The results revealed the preferable degradation of alkanes, 1-ring aliphatics and benzenes and an enrichment of condensed cycloaliphatics and aromatics. The latter compounds are known to be resistant to microbial attack.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The microbial activity of pristine and contaminated soils was investigated by measuring the following parameters: glucose induced respiration, dimethylsulphoxide reduction and the hydrolysis of fluorescein diacetate. The viable counts were determined by the plate count method.The ability of the autochthonous microorganisms of the investigated soils to degrade diesel fuel was determined in a closed system on the basis of the oxygen consumption and by direct measurements of the hydrocarbon concentrations.As expected, compost showed the highest microbial activity with regard to all three parameters, followed by the grassland and the arable soil samples which were also found to have high activity. However, soils that had been exposed to mineral oil for a long period of time showed significantly lower values.Microorganisms from contaminated sites had a high degradation potential; few pristine soils reached similar turnover rates. The investigations showed that the level of the degradation of diesel fuel in pristine soils correlated with their microbial activity, but this correlation was not found in the investigated contaminated soils.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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