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  • 1
    Electronic Resource
    Electronic Resource
    Pleiotropic and differential phenotypic expression of two sn (snowflake) mutant alleles of Neurospora crassa: analysis in homokaryotic and heterokaryotic cells (1993)
    Springer
    Current genetics 23 (1993), S. 129-133 
    Details
    ISSN: 1432-0983
    Keywords: Fungal growth ; Neurospora crassa ; Snowflake ; Heterokaryons
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutations sn (snowflake) JL301 and C136, in the centromere region of linkage group I in Neurospora crassa, are at 0.6–3.0 map units to the left of the os-4 locus. Strains carrying snJL301 produce very short aerial hyphae and only arthroconidia, and do not grow in high salt media. snC136 strains produce aerial hyphae, with abnormally large and rounded blastoconidia, at the top of the agar slant cultures, and revert to wild-type growth in high salt media. Studies with forced primary heterokaryons indicate that snJL301 is recessive while snC136 is a semi-dominant and gene-dose dependent allele, with respect to the wild-type. Taken together the results show that: (1) the sn mutations are allelic with a differential pleiotropic phenotype, and (2) snC136 may code for a partially functioning gene product while sn-JL301 appears to be a null allele.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00352011
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Antibodies against the 59 kDa polypeptide of the N. crassa 8–10 nm filaments immunodetect a 59 kDa polypeptide in specialized rat epithelial cells (1991)
    Springer
    Molecular and cellular biochemistry 106 (1991), S. 125-131 
    Details
    ISSN: 1573-4919
    Keywords: neurospora crassa filaments ; intermediate filaments ; rat tongue epithelial polypeptide ; epithelial cell differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract P59Nc is a polypeptide associated with bundles of cytoplasmic and nuclear filamentous structures of 8–10 nm of diameter in Neurospora crassa cells. It is immunologically unrelated to both higher and lower eucaryotic tubulin and actin proteins and is detected weakly by the anti IFA monoclonal antibody. We analyze here the immunological relationship between P59Nc and intermediate filament (IF) mammalian proteins by using anti P59Nc, anti keratin, anti vimentin and anti IFA antibodies. Anti P59Nc antibodies detected a 59 kDa polypeptide from rat and bovine tissues which copurifies with polypeptides of the IF family. Neither P59Nc nor the 59 kDa rat polypeptide were recognized by anti keratin or anti vimentin antibodies. Immunostaining of rat tongue sections with anti P59Nc antibodies showed that the 59 kDa rat polypeptide is present in the cortical cytoplasm of suprabasal epithelial cells. The results indicate that P59Nc shares common antigenic determinants with a still uncharacterized 59 kDa polypeptide from mammalian tissues which have extractability and immunological properties similar to those of IF polypeptides and shows a tissular distribution and a cellular localization similar but distinguishable from keratins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230178
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Clathrin coated vesicles in Neurospora crassa (1987)
    Springer
    Molecular and cellular biochemistry 77 (1987), S. 63-70 
    Details
    ISSN: 1573-4919
    Keywords: clathrin ; coated vesicles ; N. crassa coated vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Electropherograms of Neurospora crassa homogenates showed a polypeptide with a mobility slightly lower than that of a standard sample of clathrin (from bovine brain). Subcellular fractionation of the homogenate resulted in a 20-fold enrichment of the putative N. crassa clathrin in the microsomal fraction. Further fractionation of the microsomal fraction by glass bead permeation chromatography yielded a fraction enriched about 150-fold relative to the homogenate. Coated vesicles (42.5 ± 2.5 nm diameter) were found in this preparation by electron microscopy of negatively stained specimens. Ribosomes were virtually absent from this sample. N. crassa clathrin remained associated with the coated vesicles after repeated centrifugation and homogenization steps, even in the presence of 0.4 M-NaCl, but was released by treatment with Tris buffer pH 8.5. However the polypeptide was again sedimentable after dialysis against Mes buffer pH 6.5. Under the electron microscope this sediment resembled the empty coats of higher eukaryotes. The results taken together indicate that a clathrin-like protein occurs in wild type cells of N. crassa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00230151
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    In vivo cloning of DNA into multicopy cosmids by mini-Mu-cosduction (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 546-549 
    Details
    ISSN: 1617-4623
    Keywords: Transposition ; Genomic libraries ; Recombinant DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A general in vivo procedure for cloning Escherichia coli genes into cosmids has been developed. The method we describe here uses a deleted Mu phage (a mini-Mu) to transpose E. coli genes into cosmids during mini-Mu replication. The resulting cosmids clones are packaged in-vivo into λ phage particles. Plasmids carrying a particular DNA sequence can be selectively recovered after infection of a new host with the in vivo constructed genomic cosmid library. This system was used succesfully to clone several E. coli genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338096
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    Paper (German National Licenses)
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