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Digitale Medien

Subcellular Distribution of Opioid Peptides in Rat Hypothalamus and Pituitary (1984)

Molineaux, C. J. ; Rosenberger, J. G. ; Cox, B. M.

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 43 (1984), S. 0

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ISSN: 1471-4159

Quelle: Blackwell Publishing Journal Backfiles 1879-2005

Thema: Medizin

Notizen: Homogenates of rat anterior lobe (AL) and neurointermediate lobe (NIL) pituitary and rat hypothalamus were subjected to subcellular fractionation and density gradient centrifugation. The subcellular distribution of immunoreactive dynorophin A (ir-Dyn A) in NIL was found to be similar to that of ir-arginine vasopressin (ir-AVP). ir-Dyn A migrated as a discrete band on sucrose density gradients, which corresponded in sedimentation rate to that of ir-AVP, suggesting that these two peptides are stored within organelles of similar size and density. Two other products of prodynorphin, ir-α-neoendorphin ir-α-nEND) and ir-Dyn A-(1–8) also comigrated with ir-AVP. ir-[Leu5]-enkephalin (ir-LE), which may be a product of prodynorphin or proenkephalin, was also found to migrate in this region of the gradient. When a homogenate of rat hypothalamus was prepared using a method that has been developed for synaptosome isolation, ir-Dyn A was found to comigrate with Na+/K+-activated adenosine triphosphatase (Na/K-ATPase), a synaptosomal marker enzyme. Using a more concentrated homogenate ir-Dyn A was found to migrate to a less dense region where peptide-containing synaptic vesicles have previously been localized. When a synaptosomal preparation was lysed in hypotonic solution a shift was seen in the migration rate of (ir-Dyn A to this region of the gradient (containing putative synaptic vesicles). Thus the bulk of hypothalamic dynorphin appears to be present within synaptosotne-like structures which, upon lysis, release a less dense, smaller subcellular organelle corresponding in sedimentation characteristics to other types of peptide-containing synaptic vesicles. Using either method ir-AVP migrated to a position that was different from that of ir-Dyn A and was unaffected by lysis conditions. These results indicate that the majority of the dynorphin in the hypothalamus is not stored with vasopressin at the subcellular level. Immunoreactivities associated with Dyn A-(4–8), α-nEND, LE, and [Met5]-en-kephalin-Arg6-Phe7 (ME-RF) in hypothalamus were all found to migrate with ir-Dyn A and all are shifted to approximately the same location by lysis. Thus all of the prodynorphin and proenkephalin products examined appear to be stored within synaptic vesicle-like organelles present primarily within nerve terminals in the hypothalamus.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1111/j.1471-4159.1984.tb06086.x

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Digitale Medien

151. Langzeitergebnisse nach chirurgischen Eingriffen wegen chronischer Pankreatitis (1980)

Rosenberger, J. ; Junginger, Th. ; Mödder, U. ; [weitere]

Springer

Langenbeck's archives of surgery 352 (1980), S. 520-521

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ISSN: 1435-2451

Schlagwort(e): Pancreas follow-up ; Computer tomography ; Point system ; Pankreasnachsorge ; Computertomographie ; Punktesystem

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Beschreibung / Inhaltsverzeichnis: Zusammenfassung Von 126 Patienten wiesen 46% einen Diabetes mellitus auf, der in 27% z. Z. der Nachuntersuchung unbekannt oder schlecht eingestellt war. Eine exokrine Insuffizienz wurde bei 67% der Patienten gesehen, die in 28% der Fälle gar nicht oder unzureichend behandelt wurde. 21% waren Frührentner oder längerdauernd arbeitslos, wobei 7mal eine Frühinvalidisierung nicht gerechtfertigt war. Treten nach Pankreasoperationen Oberbauchbeschwerden auf, so lassen sich mit Hilfe der Computertomographie verläßliche Aussagen über Veränderungen machen. Zur besseren Vergleichbarkeit der OP-Ergebnisse wird ein Punktesystem vorgeschlagen.

Notizen: Summary Of 126 patients, 46% had diabetes mellitus which was unknown or badly diagnosed at the time of the check-up. We saw exocrine insufficiency in 67% who had not been treated at all or just insufficiently in 28% of all cases. Premature pensioners or patients who had been unemployed for a prolonged period made up 21%, and premature disability was unjustified in 7 cases. If ailments in the epigastrium occur, reliable statements on alterations can be made with the aid of computer tomography. A point system is proposed for better comparison of operative results.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01292114

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