ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
The incorporation of 14C-labelled phenylalanine into proteins of mitochondria prepared from 48-h germinating seeds of Vigna sinensis (L.) Savi can be stimulated by gibberellic acid (GA3), kinetin and polyuridylic acid [poly(U)]. The incorporation is sensitive to various metabolic inhibitors which include rifampicin, actinomycin D, ethidium bromide, kanamycin, 5-azauracil, 8-azaadenine, and rotenone. They were used to study the effect of GA3 and kinetin on the sequential steps in protein synthesis (DNA -〉 RNA -〉 protein), which are variously affected by the inhibitors. The inhibitory effect of actinomycin D on the incorporation can be reversed by GA3 and kinetin, which implies that these two plant hormones act at the transcription level. With ethidium bromide the effect of hormones is not so convincing, though the trend is towards some release of inhibition. The plant hormones have apparently no effect on the incorporation sensitive to other inhibitors studied.GA3 and kinetin can also stimulate the normal and polyadeny-lic acid [poly(A)]-directed incorporation of 14C-labelled lysine into proteins by a mitochondria! preparation, lndole-3-acetic acid and 2,4-dichlorophenoxyacetic acid can only stimulate the normal synthesis, which is not further stepped up by poly (A). The polynucleotide even seems to depress the action of IAA. This anomaly has been discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1974.tb03676.x
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