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  • 1
    Electronic Resource
    Electronic Resource
    Structural analyses of lipid A from lipopolysaccharides of nodulating and non-nodulating Rhizobium trifolii (1985)
    Springer
    Archives of microbiology 141 (1985), S. 284-289 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium trifolii ; Lipopolysaccharide ; Lipid A-structure ; “Nod” plasmids ; 3-Hydroxy fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Structural studies have been performed on lipid A preparations derived from lipopolysaccharides of nodulating (nod+) Rhizobium trifolii wild type strains and non-nodulating (nod-) mutants deprived of the medium size “Nod” plasmid. All preparations contain a lipid A backbone composed of a β1,6-linked d-glucosamine disaccharide (GlcN II-β1,6-GlcN I) which is bis-phosphorylated at positions 1 and 4′. The phosphate group at position 4′ (GlcN II) is nonstoichiometrically substituted probably by a neutral glycosyl residue. Another substituent (probably also a neutral sugar) substitutes partly position 4 (GlcN I) of the disaccharide. The hydroxyl groups at positions 3 and 3′ are likely to be esterified by fatty acyl residue. In lipid A of nod+ strains, 3-hydroxyhexa- and octadecanoic acid are linked to the amino group of GlcN I, and 3-hydroxyhexa- and tetradecanoic acid to the amino group of GlcN II. In lipid A of nod- strains, mainly 3-hydroxyhexadecanoic acid is linked to the amino group of GlcN I, and 3-hydroxytetra- and hexadecanoic acid to that of GlcN II. The results show that rhizobial lipid A expresses many similarities to the lipid A of Enterobacteria. The structure shows, however, also peculiarities, especially regarding substituents of the lipid A backbone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00428838
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  • 2
    Electronic Resource
    Electronic Resource
    Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213 (1995)
    Springer
    Archives of microbiology 163 (1995), S. 345-351 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsRhizobium loti ; Rhizobium huakuii ; Lipopolysaccharide ; 4-Oxo-20:0 ; 6-Deoxy-l-talose ; 2 ; 3-Diamino-2 ; 3-dideoxy-d-glucose ; Lipid ADAG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phenol-water extraction of Rhizobium loti NZP2213 cells allowed a simultaneous isolation of two structurally different lipopolysaccharides from the aqueous (LPS-W) and phenol (LPS-P) phase that differed in their sodium deoxycholate-PAGE pattern and composition. LPS-W showed a profile indicating an R-type LPS; LPS-P had a cluster of poorly resolved bands in the high-molecular-weight region. LPS-P contained large amounts of 6-deoxy-l-talose (6dTal), and a small amount of 2-O-methyl-6-deoxy-talose (molar ratio ∼30:1), both of which were completely absent in LPS-W. Methylation analysis gave only one major product, 2,4-di-O-methyl-6dTal, indicating that the O-chain is composed of a homopolymer of 1,3-linked 6dTal, having the methylated 6dTal (2-O-Me-6dTal) probably localized at the non-reducing end of the O-chain. This homopolymeric O-chain was additionally O-acetylated, as evidenced by GC-MS and by 13C NMR analysis. The lipid A moieties of both LPS-W and LPS-P showed almost identical composition, with six different 3-OH fatty acids and with two, so far not described, long-chain 4-oxo-fatty acids, all being amide-linked, and with 27-OH-28:0 as the main ester-linked fatty acid. Lipid A was of the lipid ADAG-type, i.e., having a (phosphorylated) 2,3-diamino-2,3-dideoxy-d-glucose-containing lipid A backbone. Lipid ADAG is widespread among species of the α-2 group of Proteobacteria, but has so far not been encountered in any other rhizobial or agrobacterial species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404207
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the lipopolysaccharides from Rhizobium meliloti strain 102F51 and its nonnodulating mutant WL113 (1996)
    Springer
    Archives of microbiology 165 (1996), S. 26-33 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsRhizobium meliloti ; Lipopolysaccharide ; 3-Deoxyheptulosaric acid ; 2-Keto-3-deoxyoctonic acid ; Core oligosaccharide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lipopolysaccharides from the Rhizobium meliloti wild-type strain 102F51, which is effective in symbiosis with alfalfa, and from the nonnodulating mutant WL113, defective in root hair adhesion, derived thereof, were isolated and comparatively analyzed. Both preparations were composed of galactose, glucose, glucuronic acid, galacturonic acid, glucosamine, 3-deoxyheptulosaric acid, and 2-keto-3-deoxyoctonic acid as the major sugar constitutents. After a modified methylation analysis (consisting of the following consecutive steps: methylation, carboxyl reduction, remethylation, mild acid hydrolysis, reduction, and trideuterio-methylation), all of the 3-deoxyheptulosaric and some of the 2-keto-3-deoxyoctonic acid residues were converted into their corresponding 3-deoxyalditol derivatives, which carried trideuteriomethyl groups at positions C-2, C-4, and C-6. Another part of the permethylated 3-deoxyoctitol was also found as 2,5,6- and 2,6,8-tri-O-trideuteriomethyl derivatives. NMR data obtained with the separated oligosaccharides and the results of methylation analysis indicated that the majority of 2-keto-3-deoxyoctonate was present in the fraction of permethylated disaccharide alditols, namely as 6-O-CD3-aGlc(1→5)3-deoxyoctitol, 6-O-CD3-βGlcNMeAcyl(1→4)3-deoxyoctitol, and as the permethylated trisaccharide alditol, αGalA(1→3)-[6-O-CD3]-β-Glc(1→5)-[4-O-CD3]-3-deoxyoctitol. The presence of trideuteriomethyl groups at C-4 of both 3-deoxyalditols and at C-6 of the glucosaminyl or glucosyl residues indicated the linkage points of the released acid-labile ketosidic substituents, such as 3-deoxyheptulosarate and 2-keto-3-deoxyoctonate, in these oligosaccharides. The main differences between the preparations from the wild-type 102F51 and its mutant strain WL 113 were found in the higher content (in strain 102F51) of the following oligosaccharides: α-glucuronosyl(1→4)2-keto-3-deoxyoctonate and α-galacturonosyl-(1→3)α-glucosyl-(1→5)2-keto-3-deoxyoctonate and in the decreased content of β-glucosaminyl(1→4)2-keto-3-deoxy-octonate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050292
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  • 4
    Electronic Resource
    Electronic Resource
    Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213 (1995)
    Springer
    Archives of microbiology 163 (1995), S. 345-351 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium loti ; Rhizobium huakuii ; Lipopolysaccharide ; 4-Oxo-20:0 ; 6-Deoxy-l-talose ; 2,3-Diamino-2,3-dideoxy-d-glucose ; Lipid ADAG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phenol-water extraction of Rhizobium loti NZP2213 cells allowed a simultaneous isolation of two structurally different lipopolysaccharides, from the aqueous (LPS-W) and phenol (LPS-P) phase that differed in their sodium doexycholate-PAGE pattern and composition. LPS-W showed a profile indicating an R-type LPS; LPS-P had a cluster of poorly resolved bands in the high-molecular-weight region. LPS-P contained large amounts of 6-deoxy-l-talose (6dTal), and a small amount of 2-O-methyl-6-deoxy-talose (molar ratio ≈30:1), both of which were completely absent in LPS-W. Methylation analysis gave only one major product, 2,4-di-O-methyl-6dTal, indicating that the O-chain is composed of a homopolymer of 1,3-linked 6dTal, having the methylated 6dTal (2-O-me-6dTal) probably localized at the non-reducing end of the O-chain. This homopolymeric O-chain was additionally O-acetylated, as evidenced by GC-MS and by 13C NMR analysis. The lipid A moieties of both LPS-W and LPS-P showed almost identical composition, with six, different 3-OH fatty acids and with two, so far not described, long-chain 4-oxo-fatty acids, all being amide-linked, and with 27-OH-28:0 as the main ester-linked fatty acid. Lipid A was of the lipid ADAG-type, i.e., having a (phosphorylated) 2,3-diamino-2,3-dideoxy-d-glucose-containing lipid A backbone. Lipid ADAG is widespread among species of the α-2 group of Proteobacteria, but has so far not been encountered in any other rhizobial or agrobacterial species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404207
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  • 5
    Electronic Resource
    Electronic Resource
    Effect of excess Cu on the photosynthetic apparatus of runner bean leaves treated at two different growth stages (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 91 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Runner bean plants (Phaseolus coccineus L., cv Piekny Jas) were treated with excess Cu (20 mg l−1 in the form of CuSO4.5H2O) at different stages of growth to investigate, 10 days after the element treatment, the effect of Cu on acyl lipid and polypeptide composition of the thylakoid membranes and their PSII photochemistry. The plants treated with Cu in the initial stage of leaf growth showed a strong reduction in the area and fresh weight of the primary leaves. The concentration of chlorophyll and acyl lipids slightly increased when calculated on leaf area or fresh weight basis. The decrease in individual acyl lipid classes expressed on chlorophyll basis was accompanied by lower accumulation of some extrinsic polypeptides of the oxygen evolving complex and decrease in PSll activity (80% of control). Chlorophyll a fluorescence measurements suggest an inhibitory effect of Cu on the acceptor side of PSII due to induced inhibition of the Calvin cycle and down-regulation of electron transport. However, plants treated with Cu by the end of the intensive growth stage of the primary leaves showed chlorosis and almost unchanged leaf area. Moreover, significant changes in acyl lipid content as well as a distinct loss of core antenna PSII polypeptides and oxygen evolving complex subunits were observed. Changes in chlorophyll a fluorescence parameters of the thylakoid membranes suggest that low PSII activity (50% of control) may result from an alteration both in the acceptor and donor sides of PSII and its reaction centre. The growth stage of plants in which Cu was applied to plants and the duration of Cu action appears to be of great importance for the interpretation of experimental data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb03010.x
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  • 6
    Electronic Resource
    Electronic Resource
    Plasmid-determined alterations of Salmonella typhimurium lipopolysaccharides (1975)
    Springer
    Molecular genetics and genomics 140 (1975), S. 175-181 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Salmonella typhimurium Rc902 infected with derepressed ColIb mutants gave rise to changes in the composition of bacterial lipopolysaccharides (LPS). Bacteria carrying ColIbdrd7, derepressed in transfer, exhibited a marked decrease in the content of all 0-side-chain sugars of LPS. Similar effects were found upon the introduction of R64-11, also derepressed in transfer. In LPS of S. typhimurium containing ColIbdrd2, derepressed in colicin synthesis, a decrease of abequose content associated with an increase of glucose level was observed. Bacteria carrying the wild-type ColIb, the revertant of a drd mutant to the wild type, or the non colicinogenic strain resulting from the elimination of ColIbdrd2, showed no changes in the sugar composition of LPS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00329785
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  • 7
    Electronic Resource
    Electronic Resource
    A New Mesorhizobium loti HAMBI 1129 Phage Isolated from Polish Soil (2000)
    Springer
    Current microbiology 40 (2000), S. 341-343 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Phage A1 isolated from the rhizosphere of Lotus corniculatus was studied. It had a very narrow host range, as it was active only against Mesorhizobium loti HAMBI 1129. Phage A1 was classified as belonging to C Bradley's group bacteriophages. The latent period of A1 was 120–130 min and a burst size 13–17 particles per cell. The nature of the phage receptor was examined. Lipopolysaccharide from the phage-sensitive strain inactivated phage A1 in contrast to LPS from the phage-resistant bacteria. Purified LPS obtained from M. loti HAMBI 1129 had a high receptor activity with PhI50 value of 0.025 μg/ml.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002849910067
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