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  • 1
    Electronic Resource
    Electronic Resource
    Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213 (1995)
    Springer
    Archives of microbiology 163 (1995), S. 345-351 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsRhizobium loti ; Rhizobium huakuii ; Lipopolysaccharide ; 4-Oxo-20:0 ; 6-Deoxy-l-talose ; 2 ; 3-Diamino-2 ; 3-dideoxy-d-glucose ; Lipid ADAG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phenol-water extraction of Rhizobium loti NZP2213 cells allowed a simultaneous isolation of two structurally different lipopolysaccharides from the aqueous (LPS-W) and phenol (LPS-P) phase that differed in their sodium deoxycholate-PAGE pattern and composition. LPS-W showed a profile indicating an R-type LPS; LPS-P had a cluster of poorly resolved bands in the high-molecular-weight region. LPS-P contained large amounts of 6-deoxy-l-talose (6dTal), and a small amount of 2-O-methyl-6-deoxy-talose (molar ratio ∼30:1), both of which were completely absent in LPS-W. Methylation analysis gave only one major product, 2,4-di-O-methyl-6dTal, indicating that the O-chain is composed of a homopolymer of 1,3-linked 6dTal, having the methylated 6dTal (2-O-Me-6dTal) probably localized at the non-reducing end of the O-chain. This homopolymeric O-chain was additionally O-acetylated, as evidenced by GC-MS and by 13C NMR analysis. The lipid A moieties of both LPS-W and LPS-P showed almost identical composition, with six different 3-OH fatty acids and with two, so far not described, long-chain 4-oxo-fatty acids, all being amide-linked, and with 27-OH-28:0 as the main ester-linked fatty acid. Lipid A was of the lipid ADAG-type, i.e., having a (phosphorylated) 2,3-diamino-2,3-dideoxy-d-glucose-containing lipid A backbone. Lipid ADAG is widespread among species of the α-2 group of Proteobacteria, but has so far not been encountered in any other rhizobial or agrobacterial species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404207
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  • 2
    Electronic Resource
    Electronic Resource
    The structure of the lipid A component of Sphaerotilus natans (1991)
    Springer
    Archives of microbiology 156 (1991), S. 167-175 
    Details
    ISSN: 1432-072X
    Keywords: Sphaerotilus natans ; Lipopolysaccharide ; Lipid A ; Laser desorption mass spectrometry ; DOC-PAGE ; 3-Hydroxycapric acid ; Proteobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lipopolysaccharide of Sphaerotilus natans afforded a ladder-like pattern of bands in sodium deoxycholate-polyacrylamide gel electrophoresis, indicating the presence of a S-form lipopolysaccharide. The chemical analysis showed neutral sugars (rhamnose, glucose, l-glycero-d-manno-heptose), 3-deoxy-octulosonic acid (Kdo), amino compounds (glucosamine, glucosamine phosphate, ethanolamine and ethanolamine phosphate), and phosphorus. The lipid A fraction contained saturated and unsaturated capric, lauric, and myristic acids, and 3-hydroxy capric acid (3-OH-10:0). Its chemical structure was consisting of a glucosamine disaccharide, glycosidically substituted by a phosphomonoester, and substituted at C-4′ by a pyrophosphodiester esterified with ethanolamine. The amino groups of both glucosamines are acylated by 3-hydroxy capric acids and these in turn are substituted by saturated and unsaturated capric, lauric, and myristic acids. Hydroxyl groups of the backbone disaccharide at C-3 and C-3′ were also esterified by 3-hydroxy capric acid, those at C-4 and C-6 were unsubstituted. The latter provides the attachment site for Kdo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249110
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the lipopolysaccharides from Rhizobium meliloti strain 102F51 and its nonnodulating mutant WL113 (1996)
    Springer
    Archives of microbiology 165 (1996), S. 26-33 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsRhizobium meliloti ; Lipopolysaccharide ; 3-Deoxyheptulosaric acid ; 2-Keto-3-deoxyoctonic acid ; Core oligosaccharide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lipopolysaccharides from the Rhizobium meliloti wild-type strain 102F51, which is effective in symbiosis with alfalfa, and from the nonnodulating mutant WL113, defective in root hair adhesion, derived thereof, were isolated and comparatively analyzed. Both preparations were composed of galactose, glucose, glucuronic acid, galacturonic acid, glucosamine, 3-deoxyheptulosaric acid, and 2-keto-3-deoxyoctonic acid as the major sugar constitutents. After a modified methylation analysis (consisting of the following consecutive steps: methylation, carboxyl reduction, remethylation, mild acid hydrolysis, reduction, and trideuterio-methylation), all of the 3-deoxyheptulosaric and some of the 2-keto-3-deoxyoctonic acid residues were converted into their corresponding 3-deoxyalditol derivatives, which carried trideuteriomethyl groups at positions C-2, C-4, and C-6. Another part of the permethylated 3-deoxyoctitol was also found as 2,5,6- and 2,6,8-tri-O-trideuteriomethyl derivatives. NMR data obtained with the separated oligosaccharides and the results of methylation analysis indicated that the majority of 2-keto-3-deoxyoctonate was present in the fraction of permethylated disaccharide alditols, namely as 6-O-CD3-aGlc(1→5)3-deoxyoctitol, 6-O-CD3-βGlcNMeAcyl(1→4)3-deoxyoctitol, and as the permethylated trisaccharide alditol, αGalA(1→3)-[6-O-CD3]-β-Glc(1→5)-[4-O-CD3]-3-deoxyoctitol. The presence of trideuteriomethyl groups at C-4 of both 3-deoxyalditols and at C-6 of the glucosaminyl or glucosyl residues indicated the linkage points of the released acid-labile ketosidic substituents, such as 3-deoxyheptulosarate and 2-keto-3-deoxyoctonate, in these oligosaccharides. The main differences between the preparations from the wild-type 102F51 and its mutant strain WL 113 were found in the higher content (in strain 102F51) of the following oligosaccharides: α-glucuronosyl(1→4)2-keto-3-deoxyoctonate and α-galacturonosyl-(1→3)α-glucosyl-(1→5)2-keto-3-deoxyoctonate and in the decreased content of β-glucosaminyl(1→4)2-keto-3-deoxy-octonate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050292
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  • 4
    Electronic Resource
    Electronic Resource
    Chemical studies on the lipopolysaccharide of Rhizobium meliloti 10406 and its lipid A region (1989)
    Springer
    Archives of microbiology 152 (1989), S. 527-532 
    Details
    ISSN: 1432-072X
    Keywords: Fast growing rhizobia ; Lipid A ; 3-Hydroxy-octadecenoic acid ; 27-Hydroxyoctacosanoic acid ; Rhizobium meliloti
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The structure of the lipopolysaccharide from Rhizobium meliloti 10406, a derivative of the wild-type strain MVII-1, was examined. The compositional analysis of its polysaccharide moiety demonstrated lack of heptose(s), but high contents in glucose, galacturonic acid and 2-keto-3-deoxy-octonate (dOclA) as characteristic features. The lipid A moiety consisted of a β-1,6 linked glucosamine disaccharide carrying ester (at C-4′) and glycosidically (at C-1) linked phosphate residues, both present exclusively as monoester phosphates but not as phosphodiesters. Ester- and amidelinked 3-hydroxy fatty acids were mostly present as non-3-O-acylated residues. Laser desorption mass spectrometry (LD-MS) revealed heterogeneity in the fatty acid substitution, as was also indicated by the non-stoichiometric ratios obtained by quantitative fatty acid analysis. The predominating lipid A structure contained at the reducing glucosamine residue ester-linked 3-hydroxy-tetradecanoic acid (3-OH-14:0) and amide-linked 3-OH-18:0, or 3-OH-18:1, respectively. The distal (non-reducing) glucosamine carried ester-bound the recently discovered 27-hydroxyoctacosanoic acid and 3-OH-14:0 and, as amide-linked fatty acid, mostly 3-hydroxy-stearic acid (3-OH-18:0). The isolated lipopolysaccharide exhibited a high extent of lethal toxicity in galactosamine-treated mice, comparable to that of enterobacterial lipopolysaccharide. The structural relationship of LPS and lipid A of Rhizobium meliloti to other rhizobial lipopolysaccharides and lipid A's with respect to questions of taxonomy and of phylogenetic relationships will be discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425481
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  • 5
    Electronic Resource
    Electronic Resource
    Chemical characterization of two lipopolysaccharide species isolated from Rhizobium loti NZP2213 (1995)
    Springer
    Archives of microbiology 163 (1995), S. 345-351 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium loti ; Rhizobium huakuii ; Lipopolysaccharide ; 4-Oxo-20:0 ; 6-Deoxy-l-talose ; 2,3-Diamino-2,3-dideoxy-d-glucose ; Lipid ADAG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phenol-water extraction of Rhizobium loti NZP2213 cells allowed a simultaneous isolation of two structurally different lipopolysaccharides, from the aqueous (LPS-W) and phenol (LPS-P) phase that differed in their sodium doexycholate-PAGE pattern and composition. LPS-W showed a profile indicating an R-type LPS; LPS-P had a cluster of poorly resolved bands in the high-molecular-weight region. LPS-P contained large amounts of 6-deoxy-l-talose (6dTal), and a small amount of 2-O-methyl-6-deoxy-talose (molar ratio ≈30:1), both of which were completely absent in LPS-W. Methylation analysis gave only one major product, 2,4-di-O-methyl-6dTal, indicating that the O-chain is composed of a homopolymer of 1,3-linked 6dTal, having the methylated 6dTal (2-O-me-6dTal) probably localized at the non-reducing end of the O-chain. This homopolymeric O-chain was additionally O-acetylated, as evidenced by GC-MS and by 13C NMR analysis. The lipid A moieties of both LPS-W and LPS-P showed almost identical composition, with six, different 3-OH fatty acids and with two, so far not described, long-chain 4-oxo-fatty acids, all being amide-linked, and with 27-OH-28:0 as the main ester-linked fatty acid. Lipid A was of the lipid ADAG-type, i.e., having a (phosphorylated) 2,3-diamino-2,3-dideoxy-d-glucose-containing lipid A backbone. Lipid ADAG is widespread among species of the α-2 group of Proteobacteria, but has so far not been encountered in any other rhizobial or agrobacterial species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404207
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Effect of excess Cu on the photosynthetic apparatus of runner bean leaves treated at two different growth stages (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 91 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Runner bean plants (Phaseolus coccineus L., cv Piekny Jas) were treated with excess Cu (20 mg l−1 in the form of CuSO4.5H2O) at different stages of growth to investigate, 10 days after the element treatment, the effect of Cu on acyl lipid and polypeptide composition of the thylakoid membranes and their PSII photochemistry. The plants treated with Cu in the initial stage of leaf growth showed a strong reduction in the area and fresh weight of the primary leaves. The concentration of chlorophyll and acyl lipids slightly increased when calculated on leaf area or fresh weight basis. The decrease in individual acyl lipid classes expressed on chlorophyll basis was accompanied by lower accumulation of some extrinsic polypeptides of the oxygen evolving complex and decrease in PSll activity (80% of control). Chlorophyll a fluorescence measurements suggest an inhibitory effect of Cu on the acceptor side of PSII due to induced inhibition of the Calvin cycle and down-regulation of electron transport. However, plants treated with Cu by the end of the intensive growth stage of the primary leaves showed chlorosis and almost unchanged leaf area. Moreover, significant changes in acyl lipid content as well as a distinct loss of core antenna PSII polypeptides and oxygen evolving complex subunits were observed. Changes in chlorophyll a fluorescence parameters of the thylakoid membranes suggest that low PSII activity (50% of control) may result from an alteration both in the acceptor and donor sides of PSII and its reaction centre. The growth stage of plants in which Cu was applied to plants and the duration of Cu action appears to be of great importance for the interpretation of experimental data.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb03010.x
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