ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Since most brain glycoproteins from β-1,4-galactosyltransferase (β-1,4-GalT) I knockout mice were galactosylated without apparent reduction the gene expression of novel β-1,4-GalTs II and V which are involved in N-linked oligosaccharide biosynthesis in addition to β-1,4-GalT I was studied during mouse brain development. Isolation and characterization of β-1,4-GalT II and V cDNAs from mouse brains indicates that they are also functioning in the brain. Northern blot analysis revealed that the β-1,4-GalT I gene is expressed mainly in mid-embryonic stages, while the expression level of β-1,4-GalT II transcript remains constant and of β-1,4-GalT V transcript increases during mouse brain development after birth. In situ hybridization revealed that β-1,4-GalT II and V signals are present in most neural cells, with a marked difference between them in the hippocampus of adult mouse brain tissue. The differential gene expression of β-1,4-GalTs I, II and V during mouse brain development could affect the differential galactosylation of brain glycoproteins, as revealed by lectin blot analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1471-4159.2001.00004.x
Permalink