ISSN:
1573-7276
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The production of protease by malignant cells has been held responsible for invasion. The effect of aprotinin, a broad-spectrum protease inhibitor, on malignant invasion was examined histologically in organotypical cocultures of precultured embryonic chick heart fragments with two human melanoma cell lines and one virus-transformed fetal mouse carcass cell line. In the presence of 400 KIU/ml aprotinin, invasion was still permissive, while in the comparison with 0·1Μg/ml vincristine, a microtubule and directional motility inhibitor and a well-documented anti-invasive agent, invasion was completely stopped. Aprotinin remained stable in the culture medium so that the presence of invasion cannot be explained by low drug concentration. It is concluded that aprotinin-sensitive proteases are not implicated in invasionin vitro in the cell types investigated, and that thisin vitro technique deserves further interest for the study of protease inhibitors in the mechanism of invasion.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01784841
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