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  • 1
    Electronic Resource
    Electronic Resource
    1H NMR study of the interaction of bacteriophage λ Cro protein with the O R 3 operator (1985)
    Springer
    European biophysics journal 12 (1985), S. 87-95 
    Details
    ISSN: 1432-1017
    Keywords: O R 3 operator ; nuclear magnetic resonance ; assignment of proton resonances ; two-dimensional nuclear Overhauser effect spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract The 17 base pair operator O R 3 oligonucleotide, which is the preferential binding site for the Cro repressor of phage λ, was studied by two-dimensional NMR spectroscopy. A sequential assignment procedure based on two-dimensional Nuclear Overhauser Effect (NOESY) and scalar coupling correlated (COSY) NMR spectroscopy, together with the knowledge of the oligodesoxynucleotide sequence, made it possible to assign the non-exhangeable base protons and the H1′ and the H2′-H2″ sugar protons of the O R 3 operator DNA. The pattern of the observed NOE connectivities is consistent with a right-handed helical DNA structure. The base and sugar proton assignments provide the necessary information for further studies of the O R 3 operator — Cro repressor interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260431
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  • 2
    Electronic Resource
    Electronic Resource
    Conserved structure and organization of B hordein genes in the Hor 2 locus of barley (1992)
    Springer
    Plant molecular biology 18 (1992), S. 453-458 
    Details
    ISSN: 1573-5028
    Keywords: B hordein genes ; barley ; PCR ; Southern blot analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This work presents new information on the structure and organization of B hordein genes in the Hor 2 locus of barley. Data obtained by Southern blot analysis and cloning and sequencing of different members of this multigene family are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040661
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Expression of env sequences of the bovine leukemia virus (BLV) in the yeast Saccharomyces cerevisiae (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 4 (1988), S. 47-59 
    Details
    ISSN: 0749-503X
    Keywords: Bovine leukemia virus ; PH05 ; PGK ; tumor virus ; Saccharomyces cerevisiae ; viral antigens ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: DNA sequences of the envelop (env) gene of the bovine leukemia virus (BLV) were expressed in the yeast saccharomyces cerevisiae. Two yeast promoters, the responsible PH05 promoter and the constitutive PGK promoter, were used to construct four expression plasmids either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence.The expressed hetrologous gene products were characterized by Western blot analysis and competitive radio-immunoassay. By means of Northern blot analysis the steady-state level of env-specific mRNA was analysed.The highest expression rate was obtained from recombinant plasmid YEpSG 94 comprising a gp51 sequence - a 630 base pair fragment containing 70% of the gp51 but lacking the N terminus - as well as the PH05 promoter including PH05 signal sequence and the PH05 terminator. The recombinant gp51 was partially lycosylated but the PH05 signal peptide did not seem to be cleaved off. No immunoreactive material could be found in the periplasm or in the culture medium.By means of monoclonal antibodies directed against eight different epitopes of viral gp51, all for sequential antigenic determinants were detected in the AH 216(YEpSG 94) expression product.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320040106
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    Paper (German National Licenses)
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