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  • 1
    Electronic Resource
    Electronic Resource
    IgG, IgA and IgE autoantibodies against the ectodomain of desmoglein 3 in active pemphigus vulgaris (2001)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 144 (2001), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background IgG autoantibodies against desmoglein (Dsg) 3 play a key part in the pathogenesis of pemphigus vulgaris (PV), the most severe autoimmune bullous disorder. Objectives To determine whether immunoglobulin isotypes other than IgG are detectable in the sera of patients with PV and whether a particular immunoglobulin subtype is associated with a distinct clinical phenotype of PV. Methods Sera from 41 patients with acute-onset, chronic active, and remittent PV disease with mucosal and cutaneous lesions were assayed against a baculovirus-expressed Dsg3 protein by immunoblot analysis. Results In acute-onset PV, Dsg3-reactive IgG1 was detected in nine of 15 (60%), IgG4 in 14 of 15 (93%), IgA in nine of 15 (60%) and IgE in two of 15 (13%) sera. In chronic active PV, Dsg3-reactive IgG1 was detected in 11 of 18 (61%), IgG4 in 16 of 18 (89%), IgA in 13 of 18 (72%) and IgE in two of 18 (11%) sera. In contrast, sera from patients with remittent PV disease contained only Dsg3-reactive IgG1 in six of eight (75%) and IgG4 in four of eight (50%) cases, but not Dsg3-reactive IgA or IgE. Conclusions In extension of previous findings, our study demonstrates that, in addition to IgG autoantibodies, IgA and occasionally IgE autoantibodies reactive with Dsg3 are present in acute and chronic active PV. The detection of Dsg3-reactive autoantibodies of the IgG4, IgA and IgE subclasses in active PV provides additional evidence that PV is a T-helper 2-regulated autoimmune disorder.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2001.04228.x
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  • 2
    Electronic Resource
    Electronic Resource
    Regionale zerebrale Gewebesauerstoffmessung und Hirndurchblutung bei tiefer Hypothermie und Kreislaufstillstand (2000)
    Springer
    Intensivmedizin und Notfallmedizin 37 (2000), S. 113-120 
    Details
    ISSN: 1435-1420
    Keywords: Key words Deep hypothermic Curculatory arrest – ischemia-reperfusion – rabbits – brain tissue oxygen pressure –cerebral blood flow ; Schlüsselwörter Tiefe Hypothermie und Kreislaufstillstand – Ischämie-Reperfusion – Kaninchen – Hirngewebesauerstoffpartialdruck – zerebraler Blutfluß
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der vorliegenden Studie wurde ein Kaninchenmodell verwendet, um den Einfluß eines 60minütigen Kreislaufstillstandes in tiefer Hypothermie auf die intrakraniellen Veränderungen in der Verfügbarkeit von Sauerstoff im Hirngewebe sowie die Hindurchblutung untersuchen zu können. Mit der Dauer des totalen Kreislaufstillstandes nahm der Hirngewebe-Sauerstoffpartialdruck (ptiO2) kontinuierlich ab. In der Reperfusion und Erwärmung war die zerebrale Reoxygenierung mit subnormalen ptiO2-Werten stark verzögert. Am OP-Ende (nach Reperfusion und Entwöhnung von der EKZ) ergaben sich signifikant verminderte Werte für den ptiO2: 25±4 mmHg vs. Ausgangswert: 38±5 mmHg (p〈0,05) und den zerebralen Blutfluß: 43±8 ml/100 g/min vs. Ausgangswert: 68±11 ml/100 g/min (p〈0,05). Aufgrund der Ergebnisse kann angenommen werden, daß die temporär beeinträchtigte zerebrale Sauerstoffversorgungs- bzw. Utilisationsmöglichkeit mit einhergehender reduzierter Hirndurchblutung an der Pathogenese von neurologischen Störungen nach Operationen in tiefer Hypothermie und Kreislaufstillstand entscheidend beteiligt sein könnte.
    Notes: Summary We evaluated changes in brain tissue oxygen pressure (ptiO2) and global cerebral blood flow (CBF) during cardiopulmonary bypass (CPB) with deep hypothermic circulatory arrest (DHCA) in ten anesthetized New Zealand White rabbits (body weight 2.5±0.5 kg). CBF [hydrogen desaturation (clearance)-technique) and ptiO2 (Licox®-microsensor) right frontoparietal cortex] were determined. After median sternotomy, the animals were placed on CPB (standard roller pump, nonpulsatile, α-stat acid-base management, perfusion flow of 180–200 mL/kg/min, right atrial and aortic cannulation) for induction of DHCA by cooling to 15°C rectal temperature (1°C per minute). Rewarming period was started after 60 minutes of circulatory arrest and the rabbits were weaned from CPB without the use of any inotropic or vasoactive support. Under baseline conditions, ptiO2 was 38±5 mmHg and did not change significantly before DHCA. During DHCA the ptiO2 decreased within 15 minutes to 14±5 mmHg, after 30 minutes to 5±1 mmHg and after 40 minutes to zero. Neither the ptiO2 nor CBF recovered fully following restoration of bypass flow and reperfusion: ptiO2 (25±4 mmHg vs. baseline: 38±5 mmHg, p〈0.05) and CBF (43±8 vs. baseline: 68±11 ml/100 g/min, p〈0.05) were significantly reduced. Arterial and jugular-venous lactate levels increased after rewarming (p〈0.05) and corresponded to the appearance of a anaerobic metabolism. These data demonstrate significantly decreased levels of ptiO2 (brain tissue oxygenation) and CBF (oxygen supply) during and after rewarming from DHCA. The induced impairment of recovery persists after rewarming and generates a temporary low-flow situation with delayed brain tissue reoxygenation on the cellular/mitochondrial level. Thus, a direct influence on brain functional recovery by the active cooling and rewarming process about the bloodstream during CPB, resulting in disturbed tissue blood redistribution, mitochondrial dysfunction and/or excessive cerebral oxygen consumption (compensation of an oxygen debt) is a possible explanation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003900050307
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  • 3
    Electronic Resource
    Electronic Resource
    Determination of orotic acid in aqueous solutions by micellar electrokinetic capillary chromatography using sensitized lanthanide-ion luminescence detection (1996)
    Springer
    Chromatographia 42 (1996), S. 12-16 
    Details
    ISSN: 1612-1112
    Keywords: Capillary electrophoresis ; Micellar electrokinetic capillary chromatography ; Sensitized lanthanide ion luminescence ; Orotic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A sensitive and selective laser-induced luminescence detection scheme for orotic acid in urine, separated by micellar electrokinetic capillary chromatography (MEKC) has been developed. The 325 nm line from a helium cadmium laser is used to excite orotic acid, which transfers its energy to terbium. Resultant luminescence of terbium is linear with orotic acid concentration over more than 2.5 orders of magnitude. This novel and practical system enables the detection of 50 nm orotic acid in urine in less than 1.5 minutes while using only nanoliters of sample. The significant decrease in analysis time over traditional methods (spectrophotometric and chromatographic) comes from the high efficiency of MEKC. A dramatic improvement in sensitivity and selectivity over UV detection in capillary electrophoresis is achieved through the use of laser-induced lanthanide ion energy transfer luminescence detection. Finally, no sample pretreatment is needed and the method is free from any known interferences in urine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02271048
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  • 4
    Electronic Resource
    Electronic Resource
    Association of isozyme markers with quantitative trait loci in random single seed descent derived lines of lentil (Lens culinaris Medik.) (1994)
    Springer
    Euphytica 75 (1994), S. 111-119 
    Details
    ISSN: 1573-5060
    Keywords: Lens culinaris ; lentil ; gene markers ; isozymes ; quantitative traits
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Polymorphism at isozyme loci was used to locate factors responsible for variation in quantitative traits of lentil. Eight sets of random single seed descent (RSSD) derived lines were developed by advancing individual F3 plants of interspecific (L. culinaris Medik. × L. orientalis Boiss.) hybrids to the F6. The RSSD lines in each of the eight sets differed for alleles at 2–8 isozyme loci. In each set, association of isozyme loci with variation in seven quantitative traits (days to flower, days to mature, plant height, biomass, seed yield, harvest index, seed weight) was determined for each pairwise combination of a quantitative trait with a marker locus. Loci affecting variation in all seven quantitative traits were detected by their association with 14 isozyme markers (Aat-c, Aat-m, Aat-p, Adh-1, Fk, Gal-1, Gal-2, Lap-1, Lap-2, Pgd-p, Pgi, Pgm-c, Pgm-p, Skdh). The known position of 10 the 14 isozyme loci on the lentil genetic map was used to mark the genomic regions for possible location of associated quantitative trait loci (QTL). Detected QTL were found to be located in six of the seven linkage groups on lentil genetic map. Regions of the genome represented by linkage groups, 1, 5 and 7 appeared to affect a greater number of traits than other genomic regions represented by linkage groups 2, 3 and 4. Results indicated that the mean expression of quantitative traits at segregating marker locus classes can be used to locate the genetic factors in lentil which influence the behavior of economically important traits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024538
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