ISSN:
1600-0714
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The effects of recombinant human interleukin-1β (rhIL-1β) on alveolar bone resorptive activity in rats were examined. Continuous administration of rhIL-1β or phosphate-buffered saline (PBS) was given via osmotic pumps for 3, 7 and 14 days to rats with silk ligatures around second maxillary molars. Other animals without ligatures received insertion of pumps containing rhIL-lp or remained untreated. Sections were subject to three different stains:–hematoxylin and eosin (H-E) for histology, acid phosphatase (ACPase) activity for osteoclast detection, and immunohistochemistry using anti-rat monocyte/macrophage monoclonal antibody (ED 1). In addition, body weight, plasma calcium and phosphorus levels were monitored. The mean body weight of rats receiving rhIL-lp was significantly lower (P 〈 0.05 to P 〈 0.01) compared with untreated rats throughout the experimental period. On Day 7, plasma calcium and phosphorus levels were significantly lower in rats receiving rhIL-1β than in rats receiving PBS only (P 〈 0.05). Sections revealed a moderate inflammatory cell infiltrate reaching near the alveolar crest in both groups with ligatures on Day 3. Only rats receiving rhIL-lp exhibited enhancement of inflammatory cell invasion on Days 7 and 14. In rats receiving rhIL-lp with ligatures, numerous resorption lacunae containing ACPase-positive multinucleated giant cells (MNGCs), coinciding with ED1-positive cells, were located on the mesial side of the septum where extensive bone resorption had occurred throughout the experimental period. In animals receiving rhIL-β without ligatures, compared with untreated rats, increased ACPase-positive cells were observed on the mesial side of the septum on Day 3. In animals receiving PBS only, a few ACPase-positive cells were observed confined to the mesial regions where slight bone resorption occurred on Days 7 and 14. These results indicate that the administration of rhIL-1β accelerated alveolar bone destruction in ligature-induced periodontal tissue inflammation over a two-week period.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0714.1995.tb01212.x
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