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  • 1
    Electronic Resource
    Electronic Resource
    Determination of low levels of sulfur in organics by combustion microcoulometry (1977)
    s.l. : American Chemical Society
    Analytical chemistry 49 (1977), S. 1615-1618 
    Details
    ISSN: 1520-6882
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ac50019a039
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for microfloral succession on allochthonous plant litter in Apalachicola Bay, Florida, USA (1977)
    Springer
    Marine biology 41 (1977), S. 229-240 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in hydrolytic, respiratory,catabolic and lipid biosynthetic activities depend at least in part on successional changes in the microfloral populations of allochthonous plant litter incubated in a semi-tropical estuary. Initial colonization is by populations which have a high content of muramic acid relative to the adenosine triphosphate (ATP) and which are progressively displaced by a microflora with a lower ratio of muramic acid to ATP. Scanning electron micrography of the plant-litter microflora shows a succession of forms, with an initial bacterial colonization and its progressive displacement by more complex forms. Estimates of the microbial mass and the rates of phospholipid synthesis suggest that the detrital microflora has a relatively slow growth rate compared to its growth potential.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394909
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  • 3
    Electronic Resource
    Electronic Resource
    Differential adhesion, activity, and carbohydrate: Protein ratios ofPseudomonas atlantica monocultures attaching to stainless steel in a linear shear gradient (1990)
    Springer
    Microbial ecology 19 (1990), S. 269-278 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Biofilm formation on metallic surfaces in marine and freshwater environments often precedes corrosion and other biofouling conditions. Attachment is mediated by such environmental factors as the presence of surface conditioning films, fluid dynamics, bulk-phase nutrient levels, and surface chemistry. In this study, we utilized a Fowler Cell Adhesion Measurement Module to demonstrate that the changes in cellular concentration and composition of a monoculture ofPseudomonas atlantica biofilms on stainless steel were a function of the applied shear force. At shear forces in the range of 3–10 dynes cm−2 (1.0 liter min−1), attachment as measured by acridine orange direct microscopic counts was greatest at the higher shear forces.14C-Acetate uptake activity on the stainless steel surfaces increased with shear stress. Acetate incorporation ranged from 1×10−5 to 19×10−5 μmol cm−2 between 0.15 and 30 dynes cm−2 for 30 min uptake periods. On a per cell basis, however, activity decreased with shear, indicating a shift in metabolism. Fourier transform infrared spectroscopy revealed that protein and carbohydrate concentrations also increased with the applied shear. Increased biofilm C∶N ratios and total fatty acids were associated with the higher shear stresses. Neither radius of interaction nor biofilm age appeared to significantly influence the relationship between fluid shear and attachment and cellular composition ofP. atlantica biofilms in the range of 1–10 dynes cm−2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02017171
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  • 4
    Electronic Resource
    Electronic Resource
    An algD-bioluminescent reporter plasmid to monitor alginate production in biofilms (1994)
    Springer
    Microbial ecology 27 (1994), S. 225-239 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A broad-host range algD-lux bioluminescent reporter plasmid was developed to examine the role of exopolymer production in biofilm function. The algD-lux reporter plasmid will allow rapid on-line in situ detection of environmental factors that induce alginate biosynthesis. The algD promoter was stimulated by factors previously reported to induce alginate production, including ethanol and NaCl, and differences were observed with different nitrogen sources. With growth on minimal media with either glucose or succinate as a carbon source, succinate had a greater inductive effect on the algD promoter. An increase in light output of 1.3-fold and 1.7-fold was seen with cultures amended with 50 and 150 mM NaCI, respectively, compared to cultures with succinate alone. NaCl induction of the algD promoter was confirmed by algD RNA slot blots. Light output increased 2.0-fold and 1.7-fold with 0.25% and 0.5% ethanol, respectively, compared with controls grown with succinate only. While the rate of algD promoter response was initially similar when either NH4 or NO3 was used as a nitrogen source, NH4-grown cultures maintained a higher light output during late log phase compared to NO3-grown cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00182407
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  • 5
    Electronic Resource
    Electronic Resource
    Factors influencing the abundance and metabolic capacities of microorganisms in Eastern Coastal Plain sediments (1994)
    Springer
    Microbial ecology 28 (1994), S. 351-364 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abundance and metabolic capacities of microorganisms residing in 49 sediment samples from 4 boreholes in Atlantic Coastal Plain sediments were examined. Radiolabeled time-course experiments assessing in situ mirobial capacities were initiated within 30 min of core recovery. Acetate (1-14C- and3H-) incorporation into lipids, microbial colony forming units, and nutrient limitations were examined in aliquots of subsurface sediments. Water-saturated sands exhibited activity and numbers of viable microorganisms that were orders of magnitude greater than those of the low permeability dense clays. Increased radioisotope utilization rates were observed after 6–24-h incubation times when sediments were amended with additional water and/or nutrients. Supplements of water, phosphate, nitrate, sulfate, glucose, or minerals resulted in the stimulation of microbial activities, as evidenced by the rate of acetate incorporation into microbial lipids. Additions of water or phosphate resulted in the greatest stimulation of microbial activities. Regardless of depth, sediments that contained 〉20% clay particles exhibited lower activities and biomass densities, and greater stimulation with abundant water supplementation than did sediments containing 〉66% sands and hydraulic conductivities 〉 200 μm sec.−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00662028
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  • 6
    Electronic Resource
    Electronic Resource
    Comparison between geochemical and biological estimates of subsurface microbial activities (1994)
    Springer
    Microbial ecology 28 (1994), S. 335-349 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Geochemical and biological estimates of in situ microbial activities were compared from the aerobic and microaerophilic sediments of the Atlantic Coastal Plain. Radioisotope time-course experiments suggested oxidation rates greater than millimolar quantities per year for acetate and glucose. Geochemical analyses assessing oxygen consumption, soluble organic carbon utilization, sulfate reduction, and carbon dioxide production suggested organic oxidation rates of nano- to micromolar quantities per year. Radiotracer timecourse experiments appeared to overestimate rates of organic carbon oxidation, sulfate reduction, and biomass production by a factor of 103–106 greater than estimates calculated from groundwater analyses. Based on the geochemical evidence, in situ microbial metabolism was estimated to be in the nano- to micromolar range per year, and the average doubling time for the microbial community was estimated to be centuries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00662027
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The budding bacteria, Pirellula and Planctomyces, with atypical 16S rRNA and absence of peptidoglycan, show eubacterial phospholipids and uniquely high proportions of long chain beta-hydroxy fatty acids in the lipopolysaccharide lipid A (1988)
    Springer
    Archives of microbiology 149 (1988), S. 255-260 
    Details
    ISSN: 1432-072X
    Keywords: Fatty acid composition ; Pirellula ; Planctomyces ; Non-prosthecate, budding bacteria ; Phylogeny of eubacteria ; Lipids ; Fatty acids ; Lipopolysaccharides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fatty acids of twelve strains of budding bacteria (Planctomyces and Pirellula spp.), which have atypical 16S rRNA and do not contain peptidoglycan cell walls, were shown to contain typical diacyl polar lipids with no indication of isoprenoid ether lipids suggestive of a relationship with the archaebacteria. The major ester-linked fatty acids of the phospholipids were palmitic, palmitoleic and oleic acids, which are more typical of microeukaryotes than of eubacteria. Lipopolysaccharide lipid A (LPS) was detected; it contained major proportions of long chain normal 3-OH fatty acids (3-OH eicosanoic at 23% and 17% of the total in two strains of Planctomyces, and 3-OH octadecanoic at 18%, and 3-OH palmitic at 11% of the total in one strain of Pirellula). Major portions of long chain 3-OH fatty acids in the LPS are extremely unusual and provide another atypical property of these organisms. Each strain investigated showed a specific total fatty acid composition, reflecting the diversity in 16S rRNA nucleotide catalogues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422014
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  • 8
    Electronic Resource
    Electronic Resource
    Microscopic examination and fatty acid characterization of filamentous bacteria colonizing substrata around subtidal hydrothermal vents (1989)
    Springer
    Archives of microbiology 152 (1989), S. 64-71 
    Details
    ISSN: 1432-072X
    Keywords: Thiothrix sp. ; Beggiatoa sp. ; Sulfideoxidizing ; Polyunsaturated ; Fatty acids ; Inclusions ; Sheath ; Southern California ; Ultrastructure ; Sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microscopic examination of the whitish mat that covered the substrata around subtidal hydrothermal vents at White Point in southern California revealed a “Thiothrix-like” bacterium containing sulfur inclusions as the dominant filamentous form in this microbial community. The matlike appearance developed as a result of the closely-packed manner inwhich the basal ends of the filaments were anchored to the substrate. The dominant phospholipid fatty acids of these filaments (16:0, 16:1w7c, 18:0, 18:1w7c) were similar to those recovered from a sample of Beggiatoa isolated from a spring in Florida. Filaments from both sources contained small quantities of C18 and C20 polyunsaturated fatty acids, as well. A larger but less abundant sheathless, filamentous form, which also contained sulfur inclusions and displayed a cell wall structure similar to a previously described Thioploca strain, also colonized the substrata around the subtidal mat. The preservation methods used in the preparation of thin-sections of the subtidal mat material were found to be inadequate for defining some key cellular structures of the large filaments. Nevertheless, the results demonstrate that the filamentous bacteria comprising the microbial mat in the vicinity of the subtidal vents exhibit some of the features of the free-living filamentous microorganisms found in deep-water hydrothermal areas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00447013
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  • 9
    Electronic Resource
    Electronic Resource
    Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals (1993)
    Springer
    Archives of microbiology 159 (1993), S. 336-344 
    Details
    ISSN: 1432-072X
    Keywords: Iron ; Uranium ; Manganese ; Nitrate ; Anaerobic sediments ; Delta proteobacteria ; Aromatics ; Heavy metals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gram-negative metal-reducing microorganism, previously known as strain GS-15, was further characterized. This strict anaerobe oxidizes several short-chain fatty acids, alcohols, and monoaromatic compounds with Fe(III) as the sole electron acceptor. Furthermore, acetate is also oxidized with the reduction of Mn (IV), U (VI), and nitrate. In whole cell suspensions, the c-type cytochrome(s) of this organism was oxidized by physiological electron acceptors and also by gold, silver, mercury, and chromate. Menaquinone was recovered in concentrations comparable to those previously found in gram-negative sulfate reducers. Profiles of the phospholipid ester-linked fatty acids indicated that both the anaerobic desaturase and the branched pathways for fatty acid biosynthesis were operative. The organism contained three lipopolysaccharide hydroxy fatty acids which have not been previously reported in microorganisms, but have been observed in anaerobic freshwater sediments. The 16S rRNA sequence indicated that this organism belongs in the delta proteobacteria. Its closest known relative is Desulfuromonas acetoxidans. The name Geobacter metallireducens is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290916
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  • 10
    Electronic Resource
    Electronic Resource
    Adjustment to Cold of the Isolated Rat Heart (1967)
    [s.l.] : Nature Publishing Group
    Nature 214 (1967), S. 913-914 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The heart of the non-hibernating homeotherm is normally at a constant temperature, but here we report experiments which indicate that, when isolated, such a heart adjusts to a cold environment in a manner somewhat similar to that of the intact heart of the poikilotherm2. Fig. 1. Heart ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/214913a0
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    Paper (German National Licenses)
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