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  • 1
    Electronic Resource
    Electronic Resource
    DNA fingerprinting of Ascochyta rabiei with synthetic oligodeoxynucleotides (1991)
    Springer
    Current genetics 19 (1991), S. 483-489 
    Details
    ISSN: 1432-0983
    Keywords: DNA fingerprinting ; Synthetic oligodeoxynucleotides ; Simple repetitive sequences ; Fungal pathotypes ; Ascochyta rabiei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ascomycete fungus Ascochyta rabiei, an important pathogen of the grain legume crop chickpea (Cicer arietinum L.) in the Mediterranean region, has not been adequately characterized in molecular terms. We therefore used DNA fingerprinting, with synthetic oligodeoxynucleotides complementary to simple repetitive sequences, to pathotype different isolates of the fungus. Six single-spored A. rabiei isolates were first categorized using a host differential set of nine chickpea genotypes. Seedlings were inoculated under controlled environmental conditions, and disease severity was recorded 9 days after inoculation. DNA was extracted from in vitro-grown mycelia of the six purified fungal isolates, restricted with EcoRI, HinfI, MboII and TaqI, and fingerprinted with radiolabeled (GATA)4, (GTG)5, (CA)8, and (TCC)5, respectively. High levels of polymorphism were detected with optimal enzyme/probe combinations that allow one to discriminate between the isolates. The potential of DNA fingerprinting with simple repetitive sequences can thus be expanded to the identification of fungal races and pathotypes. The characterization of the geographic distribution and genetic variability of pathotypes will facilitate the selection of suitable host cultivars to be grown in specific regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312740
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  • 2
    Electronic Resource
    Electronic Resource
    Oligonucleotide and Amplification Fingerprinting of Wild Species and Cultivars of Banana ( Musa spp.) (1992)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 10 (1992), S. 1030-1035 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] DNA oligonucleotide and amplification fingerprinting have been successfully used to detect genetic polymorphisms in 15 representative species and cultivars of the genus Musa, comprising AA, AAA, AAAA, AAB, ABB, and BB genotypes. In–gel–hybridization of Hinf I–digested genomic ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0992-1030
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  • 3
    Electronic Resource
    Electronic Resource
    Oligonucleotide fingerprinting detects genetic diversity among Ascochyta rabiei isolates from a single chickpea field in Tunisia (1994)
    Springer
    Current genetics 26 (1994), S. 191-197 
    Details
    ISSN: 1432-0983
    Keywords: Ascochyta rabiei ; DNA fingerprinting ; Simple repetitive sequences ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary to simple repetitive sequences. According to the fingerprint patterns derived from the probes (CA)8, (CAA)5, (CAT)5 and (GATA)4, 12 different fungal haplotypes were found at various frequencies within the investigated field. Seven haplotypes were confined to one location only, four occurred at two, one at three, and none at all four locations. Most of the genetic variability originated from diversity within, rather than between, locations. In some cases, more than one haplotype was isolated from the same lesion of a single host plant. Genetic distances between isolates, as calculated from band-sharing data, varied between 0.05 and 0.22. Relatedness between the different haplotypes was evaluated by cluster analysis using UPGMA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00309547
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  • 4
    Electronic Resource
    Electronic Resource
    Oligonucleotide fingerprinting and RAPD analysis of Achillea species: Characterization and long-term monitoring of micropropagated clones (1996)
    Springer
    Plant cell reports 15 (1996), S. 647-652 
    Details
    ISSN: 1432-203X
    Keywords: Achillea ; Simple repetitive sequences ; Oligonucleotide fingerprinting ; RAPD analysis ; Clone identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two different DNA fingerprinting techniques were applied to a set of Achillea samples (Asteraceae), comprising ten taxa of the medicinally important A. millefolium group and six related species. Field-grown as well as in vitro-micropropagated plants were individually screened for abundance and polymorphism of target sequences recognized by oligonucleotide fingerprinting with 13 different microsatellite-complementary probes. While most probes revealed a high level of intra- and interspecific variability, fingerprints proved to be somatically stable in vegetatively propagated plant material. Analysis of the same samples by polymerase chain reaction with arbitrary 10-mer primers yielded less polymorphic patterns. Because of its higher discriminatory ability, oligonucleotide fingerprinting offers itself as the method of choice for the identification and discrimination of A. asplenifolia and A. roseoalba clones, as well as for monitoring their stability during micropropagation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232470
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  • 5
    Electronic Resource
    Electronic Resource
    Oligonucleotide fingerprinting and RAPD analysis of Achillea species: Characterization and long-term monitoring of micropropagated clones (1996)
    Springer
    Plant cell reports 15 (1996), S. 647-652 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: BPA: N-benzyl-9-[2-tetrahydro-pyranyl]-adenine; PCR: polymerase chain reaction; RAPD: random amplified polymorphic DNA; RFLP: restriction fragment length polymorphism; TAE buffer: 40 mM Tris acetate, 20 mM sodium acetate, 1 mM EDTA, pH 7.8 ; Keywords:Achillea– Simple repetitive sequences – Oligonucleotide fingerprinting – RAPD analysis – Clone identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Two different DNA fingerprinting techniques were applied to a set of Achillea samples (Asteraceae), comprising ten taxa of the medicinally important A. millefolium group and six related species. Field-grown as well as in vitro-micropropagated plants were indivually screened for abundance and polymorphism of target sequences recognized by oligonucleotide fingerprinting with 13 different microsatellite-complementary probes. While most probes revealed a high level of intra- and interspecific variability, fingerprints proved to be somatically stable, in vegetatively propagated plant material. Analysis of the same samples by polymerase chain reaction with arbitrary 10-mer primers yielded less polymorphic patterns. Because of its higher discriminatory ability, olignonucleotide fingerprinting offers itself as the method of choice for the identification and discrimination of A. asplenifolia and A. roseoalba clones, as well as for monitoring their stability during micropropagation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050091
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  • 6
    Electronic Resource
    Electronic Resource
    Chromosomal localization and distribution of simple sequence repeats and the Arabidopsis-type telomere sequence in the genome of Cicer arietinum L. (1998)
    Springer
    Chromosome research 6 (1998), S. 97-104 
    Details
    ISSN: 1573-6849
    Keywords: Cicer arietinum ; fluorescence in situ hybridization ; microsatellites ; telomeres
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We used fluorescence in situ hybridization to probe the physical organization of five simple sequence repeat motifs and the Arabidopsis-type telomeric repeat in metaphase chromosomes and interphase nuclei of chickpea (Cicer arietinum L.). Hybridization signals were observed with the whole set of probes and on all chromosomes, but the distribution and intensity of signals varied depending on the motif. On root-tip metaphase chromosomes, CA and GATA repeats were mainly restricted to centromeric areas, with additional GATA signals along some chromosomes. TA, A and AAC repeats were organized in a more dispersed manner, with centromeric regions being largely excluded. In interphase nuclei of the inner integument, CA and GATA signals predominantly occurred in the heterochromatic endochromocentres, whereas the other motifs were found both in eu- and heterochromatin. The distribution of the Arabidopsis-type telomeric repeat (TTTAGGG)n on metaphase chromosomes was found to be quite exceptional. One major cluster of repeats was spread along the short arm of chromosome B, whereas a second, weaker signal occurred interstitially on chromosome A. Only faint and inconsistent hybridization signals were visualized with the same probe at the chromosomal termini.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009282828236
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  • 7
    Electronic Resource
    Electronic Resource
    Characterisation of microsatellites from Actinidia chinensis (1996)
    Springer
    Molecular breeding 2 (1996), S. 117-131 
    Details
    ISSN: 1572-9788
    Keywords: Actinidia ; dinucleotide repeats ; genetic markers ; kiwifruit ; simple sequence repeats polyploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have identified a set of informative microsatellite markers for genome analysis in kiwifruit and related Actinidia species. A small-insert genomic library was constructed from Actinidia chinensis DNA, and screened for microsatellites. About 1.2% of the total colonies hybridised to a (GA)8 probe, 0.4% to (GT)8, and 0.1% to a mixture of three different trinucleotide repeat probes, (CAA)5, (GAA)5 and (CTA)5. From the DNA sequences of 35 hybridising clones, 18 primer pairs were designed, and used to amplify genomic DNA from 38 individual plants, representing 30 different accessions of ten Actinidia species. The banding patterns for most of the dinucleotide repeats showed a high degree of polymorphism in the diploid and tetraploid A. chinensis, and in the hexaploid A. deliciosa (kiwifruit). Heterozygosity levels of up to 100% were found among eight diploid accessions of A. chinensis examined, and the number of different-sized bands among all the species varied from 3 to 36 for each microsatellite. One simple CT microsatellite gave 21 bands with sizes suggesting that the number of repeats ranged from 9 to 37. The highest number of bands (36) and the largest size variation (〉100 bp) were observed with a complex microsatellite harbouring four different repeat motifs. The majority of primer pairs amplified bands from most of the ten Actinidia species tested. The most polymorphic primer pairs were used successfully to fingerprint a range of closely related varieties of kiwifruit (A. deliciosa).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441427
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  • 8
    Electronic Resource
    Electronic Resource
    Characterisation of microsatellites from Actinidia chinensis (1997)
    Springer
    Molecular breeding 3 (1997), S. 159-160 
    Details
    ISSN: 1572-9788
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009656631297
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  • 9
    Electronic Resource
    Electronic Resource
    Plant DNA fingerprinting with radioactive and digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences (1991)
    Weinheim : Wiley-Blackwell
    Electrophoresis 12 (1991), S. 159-169 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The existence of hypervariable DNA sequences in nuclear genomes, and the use of appropriate “fingerprinting” probes to detect them, has gained widespread scientific interest, and also led to multiple applications in diverse areas. Two years ago, the new technique of “DNA fingerprinting” was also introduced into the analysis and characterization of plant genomes, initially by using human or M 13 minisatellites as probes. In the present article, we demonstrate the applicability for plant DNA fingerprinting of oligonucleotide probes specific for simple repetitive DNA sequences. We show that various levels of intra- and interspecific polymorphisms can be detected; the information to be gained depends on the optimal combination of probe and species. Variety-specific patterns were obtained in several cases. Some probes revealed variability between individuals. Somatic variability was not observed. Different DNA isolation and purification procedures were tested in order to introduce a fast and easy-to-perform isolation method suitable for a large variety of plant species. Nonradioactive fingerprinting was performed using digoxigenated oligonucleotides as probes. Banding patterns obtained with radioactive and digoxigenin-based labeling techniques proved to be of similar quality.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150120211
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  • 10
    Electronic Resource
    Electronic Resource
    Oligonucleotide fingerprinting of plant and fungal genomes: A comparison of radioactive, colorigenic and chemiluminescent detection methods (1992)
    Weinheim : Wiley-Blackwell
    Electrophoresis 13 (1992), S. 115-122 
    Details
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Digoxigenated oligonucleotide probes complementary to simple repetitive DNA sequences were introduced into nonradioactive fingerprint analysis of plant and fungal DNA. The fragment patterns, obtained by blot hybridization of TaqI-restricted DNA from chickpea (Cicer arietinum) and its fungal pathogen Ascochyta rabiei with digoxigenated probes and either a colorigenic or a chemiluminescent detection method, were compared to those obtained with 32P-labeled probes. In combination with alkaline phosphatase and its chemiluminescent substrate 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane (AMPPD) digoxigenated oligonucleotides yielded clear-cut fingerprints with high signal-to-background ratios within several minutes of exposure to X-ray films. The chemiluminescence reaction remained stable for at least two weeks. A comparison of banding patterns obtained by radioactive versus digoxigenin-based hybridization and detection techniques revealed substantial differences in the relative signal intensities of bands. Both nonradioactive techniques show a tendency to “equalize” band intensity differences. Whereas 32P-labeled oligonucleotides are also applicable to in situ hybridization with DNA immobilized in dried agarose gels, gel hybridization did not work efficiently with digoxigenated probes and either substrate.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150130125
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