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  • 1
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1238
    Keywords: Key words Adult respiratory distress syndrome ; Nitric oxide ; Sheep ; Lung lavage ; Pulmonary circulation ; Pressure-flow relationship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives: Inhalation of nitric oxide (NO) selectively dilates pulmonary vessels in well-ventilated regions. Prostaglandin F2α (PGF2α) is a vasoconstrictor and is reported to enhance hypoxic pulmonary vasoconstriction. The objective of this study was to examine whether the combination of intravenous PGF2α and inhaled NO in ARDS lungs has a beneficial effect on oxygenation. Design: We investigated the effect of intravenous PGF2α infusion (0.05–10.0 μg/kg per min) with and without NO inhalation (60 ppm) on the hemodynamics and gas exchange in an ovine ARDS model, examining the pulmonary artery pressure versus the flow plot by varying cardiac output. Measurements and results: After lung lavage, NO inhalation reduced the mean pulmonary arterial pressure (MPAP) by decreasing the zero-flow pressure intercept from 10.6±3.8 (mean±SD) to 8.5±3.8 mmHg (p〈0.05) with no significant change in slope. NO inhalation improved PaO2 from 56±12 to 84±38 mmHg (p〈0.005) and reduced pulmonary shunt from 65±5 to 53±8% (Qs/Qt) (p〈0.001). The dose-dependent effects of PGF2α infusion were: (1) increased MPAP attributed to an increased slope in pulmonary artery pressure-flow plot; (2) decreased cardiac index; (3) decreased Qs/Qt with unchanged PaO2. The dose-dependent decrease in Qs/Qt after PGF2α infusion was attributed to the decreased cardiac output. Conclusions: It is suggested that inhalation of NO reduced the critical vascular pressure near alveoli without affecting upstream vessels, while infused PGF2α constricted the larger upstream pulmonary artery vessels without appreciably affecting the critical pressure. Inhalation of NO into well-ventilated lung areas shifted perfusion to well-oxygenated areas, and there was no supplemental shift in blood flow by adding an infusion of PGF2α.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1238
    Keywords: Adult respiratory distress syndrome ; Nitric oxide ; Sheep ; Lung lavage ; Pulmonary circulation ; Pressure-flow relationship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives Inhalation of nitric oxide (NO) selectively dilates pulmonary vessels in well-ventilated regions. Prostaglandin F2α (PGF2α) is a vasoconstrictor and is reported to enhance hypoxic pulmonary vasoconstriction. The objective of this study was to examine whether the combination of intravenous PGF2α and inhaled NO in ARDS lungs has a beneficial effect on oxygenation. Design We investigated the effect of intravenous PGF2α infusion (0.05–10.0 μg/kg per min) with and without NO inhalation (60 ppm) on the hemodynamics and gas exchange in an ovine ARDS model, examining the pulmonary artery pressure versure the flow plot by varying cardiac output. Measurements and results After lung lavage, NO inhalation reduced the mean pulmonary arterial pressure (MPAP) by decreasing the zero-flow pressure intercept from 10.6±3.8 (mean±SD) to 8.5±3.8 mmHg (p〈0.05) with no significant change in slope. NO inhalation improved PaO2 from 56±12 to 84±38 mmHg (p〈0.005) and reduced pulmonary shunt from 65±5 to 53±8% ( $$\dot Qs/\dot Qt$$ ) (p〈0.001). The dose-dependent effects of PGF2α infusion were: (1) increased MPAP attributed to an increased slope in pulmonary artery pressure-flow plot; (2) decreased cardiac index; (3) decreased $$\dot Qs/\dot Qt$$ with unchanged PaO2. The dose-dependent decrease in $$\dot Qs/\dot Qt$$ after PGF2α infusion was attributed to the decreased cardiac output. Conclusions It is suggested that inhalation of NO reduced the critical vascular pressure near alveoli without affecting upstream vessels, while infused PGF2α constricted the larger upstream pulmonary artery vessels without appreciably affecting the critical pressure. Inhalation of NO into well-ventilated lung areas shifted perfusion to well-oxygenated areas, and there was no supplemental shift in blood flow by adding an infusion of PGF2α.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-1238
    Keywords: Key words Cyclosporine ; Liver transplantation ; Polymorphonuclear neutrophils ; Respiratory burst ; GM-CSF ; Multiparameter flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective: Superoxide production by polymorphonuclear neutrophils (PMNs) under cyclosporin A (CsA) therapy following kidney transplantation is impaired. We investigated if the respiratory burst of PMNs is similarly depressed in patients undergoing CsA treatment following orthotopic liver transplantation (OLTx). Additionally, the in vitro influence of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the superoxide anion production was examined during the respiratory burst. Patients: 10 patients after OLTx and 10 healthy blood donors (control group). Measurements and results: PMNs were stimulated with bacteria (Escherichia coli) or a combination of tumour necrosis factor alpha (TNFα) and N-formyl-methionyl-leucyl-phenylalanine (FMLP). The respiratory burst was measured by oxidation of non-fluorescent dihydrorhodamine to the fluorescent rhodamine by means of flow cytometry. No differences in respiratory bursts from OLTx patients compared to those from healthy blood donors could be seen. Under TNFα/FMLP stimulation, the respiratory burst was significantly increased after in vitro incubation with GM-CSF (500 U ml–1) in patients following OLTx (from 58.2 to 74.5 %) as well as in the control group (from 47.4 to 61.9 %). Conclusions: Our results demonstrate that superoxide production is not impaired under CsA treatment following OLTx. The respiratory burst of these patients' PMNs can even be augmented by GM-CSF in vitro.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1076
    Keywords: Sudden infant death ; Infant mortality ; Epidemiology ; Sleep ; Sweating
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A prospective study was carried out to assess the frequency of sudden infant death (SID) in the Brussels area. The study was conducted between January and December 1987, on infants presented to the health clinics of the Brussels “Office de la Naissance et de l'Enfance” (ONE). The infants entered the study if: (a) they were born between January 1 and December 31, 1987; (b) they attended an ONE consultation in the Brussels area between January 1987 and December 1988; (c) visits were repeated monthly during the first 12 months of life, or until death occurred; and (d) standard questionnaires were completed for each visit. For 4,064 infants these four criteria were fulfilled. During the study period 7 infants died of SID. Their deaths were unexpected and remained unexplained despite post-mortem investigations. For each SID case, 10 control infants were selected from the same health clinics. Controls matched the SID cases in the following respects: (a) dates of visits; (b) sex; (c) gestational age; (d) legal age; and (e) the profession of both parents. For most of the 65 items studied no significant difference was seen between the 7 SID infants and their 70 matched controls. Only two variables significantly differentiated the two groups. Repeated fatigue during feeding was seen in 4 of the 7 SID cases, but only in 10 of the 70 control infants (FisherP=0.017). Profuse sweating during sleep was reported in 2 of the 7 SID infants, and in none of the 70 controls (FischerP=0.007). These two infants' bedclothes were repeatedly wet with sweat. Fatigue during feeding and sweating during sleep found in the 70 control infants could not be statistically differentiated from the frequency of symptoms reported for the general population of infants studied. It is concluded that the prevalence of SID in our local population was 1.72 SID cases per 1,000 infants studied. Similar figures are reported in other European and North American studies. An estimated loss of 505 potential years of life can be computed from our survey. The symptoms associated with SID in this selected population need to be validated by the prospective investigation of a larger population.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food biochemistry 15 (1991), S. 0 
    ISSN: 1745-4514
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Stereospecificity of oxygen addition distinguishes lipoxygenase initiated lipid peroxidation from nonenzymatic autoxidation that produces a racemic mixture. Similarly, the positional and stereoconfiguration of lipoxygenase metabolites of un saturated fatty acids determine their precise physiological bioactivity. Teleost fish gills produce predominantly the 12 hydroxy derivative of arachidonic and eicosapentaenoic acids. Following reaction of arachidonic acid with fish gill lipoxygenase, 12-hydroxyeicosatetraenoic acid (12-HETE) was isolated using column chromatography and HPLC. Chiral phase HPLC revealed that the single 12(S) HETE isomer was produced by the enzyme. Proton NMR further confirmed the structure as identical to the 12(S) HETE produced in mammalian platelets and lungs.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    BBA - Biochimica et Biophysica Acta 54 (1961), S. 448-454 
    ISSN: 0006-3002
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    s.l. : American Chemical Society
    Journal of the American Chemical Society 106 (1984), S. 86-92 
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of neurochemistry 39 (1982), S. 0 
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Several properties of soluble spiroperidol binding factors separated from bovine caudate nucleus have been investigated by a previously unreported procedure. Data consistent with high particle weight and rapid binding equilibration are reported for high-affinity (+)butaclamol-sensitive components of a digitonin extract. A slower sedimenting component is found that also exhibits high affinity for spiroperidol but is not sensitive to (+)butaclamol. Centrifugation of a caudate nucleus homogenate yields a supernatant that appears to contain a component that exhibits spiroperidol binding that is more sensitive to displacement by (-) than by (+)butaclamol. The procedure used effects rapid separation of bound from unbound tritiated ligand on short columns of Sephadex G-15 followed by extrusion and sectioning of the Sephadex. The radioactivity remaining with each section is determined. The procedure is very rapid; the addition of active phases or the changing of the ionic environment, which may disturb the equilibrium, is avoided; and recovery of the protein free of bound ligand is easily affected.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 74 (1993), S. 5960-5963 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Amplified spontaneous emission on the second xenon excimer continuum at 172 nm wavelength was observed in dense gas evaporated from a solid xenon surface and pumped by heavy ions. A pulsed 3.78 MeV Kr+ beam from a radio-frequency quadrupole accelerator was used for the experiment. A series of 800 μJ particle pulses each 10 ns long was focused to a small, elliptical beam spot leading to a pump power density of the order of 1 GW/g. Optical gain in the target was deduced from the angular distribution of light emitted along the target axis, and from a variation of optical intensity with gain length. A maximum gain of 26%/cm was determined using a model calculation which reproduced the experimental data.
    Type of Medium: Electronic Resource
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