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  • 1
    Electronic Resource
    Electronic Resource
    Metabolism of 2-hydroxyphenylglyoxylate by Moraxella sp. strain VS1 (1991)
    Springer
    Archives of microbiology 156 (1991), S. 213-217 
    Details
    ISSN: 1432-072X
    Keywords: 2-Hydroxyphenylglyoxylate ; Degradation ; Gentisate pathway ; Moraxella sp. VS1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A bacterium, designated as Moraxella sp., was enriched with 2-hydroxyphenylglyoxylate (2HPGA) as sole source of carbon and energy. Identified metabolites and enzyme activities determined with whole cells and extracts indicated that 2HPGA was degraded by an inducible sequence of enzymes via salicylaldehyde, salicylate, and gentisate; only minute amounts of salicylate were converted to catechol. Further evidence was obtained that permeases were necessary for the uptake of most aromatic compounds utilized for growth. For the direct determination of 2HPGA decarboxylase activity, an enzyme assay involving high-performance liquid chromatography for quantitation of the substrate was developped to study the initial step of the degradative pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249117
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  • 2
    Electronic Resource
    Electronic Resource
    Bacterial degradation of 3- and 4-carboxybiphenyl ether by Psuedomonas sp. NSS2 (1990)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 67 (1990), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Pseudomonas sp. strain NSS2 capable to grow with 3- and 4-carboxybiphenyl ether (CBPE) as only carbon source was isolated from soil samples, phenol and protocatechuate were trasiently excerted into the culture medium. Identification of both catabolotes gives evidence for the oxygenolytic cleavase of the ether bond.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1990.tb13854.x
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  • 3
    Electronic Resource
    Electronic Resource
    Degradation of benzene 1,3-disulfonate by a mixed bacterial culture (1996)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 136 (1996), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A benzene 1,3-disulfonate degrading mixed bacterial culture was isolated from the River Elbe downstream of Hamburg. The mixed culture was composed of five different bacterial strains. None of these strains grew in axenic culture with benzene 1,3-disulfonate as sole source of carbon and energy. In the presence of 4-nitrocatechol, resting cells of the mixed culture converted benzene 1,3-disulfonate to catechol 4-sulfonate. Experiments with cell-free extracts demonstrated that catechol 4-sulfonate was further metabolized via 3-sulfomuconate and 4-carboxymethyl-4-sulfobut-2-en-4-olide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08023.x
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  • 4
    Electronic Resource
    Electronic Resource
    Transformation of 3-chlorodibenzofuran by Pseudomonas sp. HH69 (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 81 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract: The dibenzofuran-degrading bacterial strain Pseudomonas sp. HH69 showed high oxidative activity towards 3-chlorodibenzofuran (3CDF). During the co-metabolic turnover of 3CDF large amounts of 4-chlorosalicylate and temporarily small amounts of salicylate were excreted. Simultaneously a yellow colour appeared due to the excretion of two polar products. Conversion of 3CDF by a mutant, derived from Pseudomonas sp. HH69 and defective in 2,3-dihydroxybiphenyl-1,2-dioxygenase led to the formation of equal quantities of 4′-chloro-2,2′,3-trihydroxybiphenyl (4′ CTHBP) and 4-chloro-2,2′,3-trihydroxybiphenyl (4CTHBP). Crude extracts of the wild type transformed 4′ CTHBP to 4-chlorosalicylate, whilst 4CTHBP was transformed to salicylate. Hence, we propose a non-selective initial attack on both aromatic rings of 3CDF and a degradative pathway for the resulting chlorotrihydroxybiphenyls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04706.x
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  • 5
    Electronic Resource
    Electronic Resource
    Catabolism of 3,5-dichlorosalicylate by Pseudomonas species strain JWS (1991)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 84 (1991), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A Pseudomonas sp. strain JWS was isolated from an enrichment culture with 3,5-dichlorosalicylate as the sole source of carbon and energy. Additionally, 3-chloro-, 5-chloro-, and 3,5-dibromosalicylate, but not 4-chlorosalicylate were mineralized by the organism. During growth on the chlorosalicylates, stoichiometric amounts of chloride were released into the culture medium. In the presence of both salicylate and 3,5-dichlorosalicylate, high activities were induced for the turnover of non-halogenated as well as halogenated salicylates. Enzyme activities assayed in crude cell extracts which are responsible for the oxidation of catechol and its halogenated derivatives as well as those for cycloisomerization of cis,cis-muconate and its 2,4-dichloro derivative provided indications for the involvement of inducible type II catechol 1,2-dioxygenase and muconate cycloisomerase in biodegradation of halogenated salicylates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1991.tb04570.x
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  • 6
    Electronic Resource
    Electronic Resource
    Initial reactions in the mineralization of 2-sulfobenzoate by Pseudomonas sp. RW611 (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 92 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Pseudomonas sp. strain RW611 utilized the ammonium salt of 2-sulfobenzoate as sole source of carbon, sulfur, nitrogen, and energy. The xenobiotic sulfo substituent was dioxygenolytically eliminated as sulfite, which was then slowly oxidized to sulfate. 2,3-Dihydroxybenzoate, which resulted from desulfonation underwent meta-cleavage, mediated by 2,3-dihydroxybenzoate 3,4-dioxygenase activity. This enzyme was inhibited by 3-chlorocatechol and 2,3,4-trihydroxybenzoate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05231.x
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  • 7
    Electronic Resource
    Electronic Resource
    Metabolism of 3-methyldiphenyl ether by Sphingomonas sp. SS31 (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 96 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The bacterium Sphingomonas sp. SS31, which was obtained from the diphenyl ether-degrading strain Sphingomonas sp. SS3 by an adaptation process, utilized 3-methyldiphenyl ether for growth in addition to diphenyl ether. The initial enzymatic attack onto this compound proceeded by a regioselective, but non-specific dioxygenation at the carbon carrying the ether bridge and the adjacent carbon of the unsubstituted as well as the methyl-substituted aromatic nucleus. Upon spontaneous decomposition, the resulting unstable hemiacetal structure yielded 3-methylphenol and catechol, or phenol, 3-methylcatechol, and 4-methylcatechol, respectively. Phenol and 3-methylphenol methylphenol were oxidized to the corresponding catechols which, after subsequent ortho-cleavage, were channeled into the oxoadipate pathway. Minor amounts of 3-(hydroxymethyl)-diphenyl ether detected in the supernatant of the culture broth gave evidence for an unproductive oxidation of the side-chain, finally leading to the nondegradble product 3-carboxydiphenyl ether.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05426.x
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  • 8
    Electronic Resource
    Electronic Resource
    Mineralization of 4-sulfophthalate by aPseudomonas strain isolated from the River Elbe (1994)
    Springer
    Biodegradation 5 (1994), S. 63-69 
    Details
    ISSN: 1572-9729
    Keywords: decarboxylation ; desulfonation ; 4,5-dihydroxyphthalate ; meta-cleavage ; protocatechuate pathway ; Pseudomonas sp. RW31 ; 4-sulfophthalate mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The bacteriumPseudomonas sp. strain RW31 isolated from the river Elbe utilized the ammonium salt of 4-sulfophthalate (4SPA) as sole source of carbon, sulfur, nitrogen, and energy and grew also with phthalate (PA) and several other aromatic compounds as sole carbon and energy source. The xenobiotic sulfo group of 4SPA was eliminated as sulfite, which transiently accumulated in the culture supernatant up to about 10 µM and was slowly oxidized to the stoichiometrical amount of sulfate. Biodegradation routes of 4SPA as well as of PA converged into the protocatechuate pathway and from found activities for the decarboxylation of 4,5-dihydroxyphthalate we deduce this compound the first rearomaticized intermediate after initial dioxygenation. Protocatechuate then underwentmeta-cleavage mediated by a protocatechuate 4,5-dioxygenase activity which was competitively inhibited by the structurally related compound 3,4,5-trihydroxybenzoate; protocatechuate accumulated in the medium up to an about 2 mM concentration. Indications for the presence of selective transport systems are presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00695215
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