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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 16 (1993), S. 63-65 
    ISSN: 1432-0789
    Keywords: Activation energy ; Continuous fertilizer application ; Temperature ; Undisturbed soils ; Urea hydrolysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Few published studies have examined the effects of a continuous fertilizer application on urea hydrolysis. In the present study we investigated the effects of 9 years of continuous application of urea and P fertilizers on urea hydrolysis in undisturbed soil samples as affected by temperature (5–45°C). Undisturbed soil samples of surface horizons (0–7 cm) were obtained with cutting rings (50 cm3) from different fertilizer-treatment plots and inserted in polyethylene bottles (with cutting rings). Each soil sample (in the cutting ring) was treated with 10 ml urea solution (0.5 mmol urea N g-1 soil) and then broght to 90% field capacity. The samples were left to equilibrate for 30 min at a temperature of 4°C, then placed in an incubator at 37°C for 6 h. The results indicated that 9 years of continuous application of urea but not P had a significant effect on urea hydrolysis in soil. There was a good correlation between temperature and urea hydrolysis in soil. Q 10 was between 1.97 and 2.08 in the temperature range 5–45°C.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytica Chimica Acta 276 (1993), S. 151-160 
    ISSN: 0003-2670
    Keywords: Flow injection ; Fluorimetry ; Pharmaceuticals ; Photochemical derivatization ; Thiamine ; Vitamin B"1
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 68 (1990), S. 1520-1525 
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The electrical and optical properties of radio frequency (rf) air plasma process-induced defect states in 50 Ω cm n/n+ epitaxial silicon are investigated by junction capacitance techniques. Capacitance-voltage measurements reveal the presence of a thin oxide layer of about 180 A(ring) on the 30-min plasma treated silicon sample. Deep level transient spectroscopy shows the existence of various defects in the sample. These consists of a dominant bulk electron trap labelled as E(0.46) at 0.46 eV below the conduction band, as well as continuously distributed interface states. The spectral dependence of the optical cross section for the defect levels were measured by deep level optical spectroscopy. A simple analysis indicates that a phonon mode ((h-dash-bar)ωp=28 meV) couples to the defect E(0.46). Its electron-phonon coupling strength is rather weak with a Franck–Condon shift of 0.04 eV. Defect E(0.46) anneals out at a fairly low temperature of about 120 °C. Etching off the oxide layer in a diluted HF solution was found to eliminate the E(0.46) defect level. This is tentatively explained as due to passivation of the defect E(0.46) by hydrogen from the HF solution.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Solid State Communications 66 (1988), S. 751-753 
    ISSN: 0038-1098
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel quantitative method for the determination of proteins in aqueous solutions has been based on the quenching of the resonance scattering light of colloidal silver chloride in the presence of proteins. The detection limits for eight kinds of proteins (BSA, HSA, egg albumin, human γ-IgG,α-chymotrypsin, E. Coli. alpsase, myoglobin, α-casein) were at about 8 ng/mL; the linear ranges of the calibration curves were 10–400 ng/mL ¶under optimal conditions,except for human γ-IgG (20–¶400 ng/mL), myoglobin (10–300 ng/mL), and α-casein (10–300 ng/mL). Three wavelengths (398 nm, 475 nm, 499 nm) were all suitable for the determination and any acidity from pH 3.0 to pH 9.0 could be chosen. A few non-protein substances at high concentration levels interfered with this method, but this problem could simply be overcome by diluting the samples before the assay. Mechanism studies showed that the quenching effect of proteins on the scattering light of colloidal silver chloride was mainly due to the coagulation of AgCl particles retarded by protein. The method was employed for the determination of total protein in human serum with sactifactory results.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 366 (2000), S. 303-306 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A fluorescence quenching method was developed for the rapid determination of DNA and RNA using magdala red as fluorescence probe. In weakly acidic ¶medium, the fluorescence of magdala red (λex〉lem = 54055 nm) can be largely quenched by DNA or RNA. The calibration graphs are linear over the range 0.01–¶1.2 μg/mL for both calf thymus DNA (CT DNA) and salmon DNA (SM DNA), and 0.015–1.0 μg/mL for yeast RNA, respectively. The corresponding detection limits are ¶6.0 ng/mL for CT DNA, 7.0 ng/mL for SM DNA and ¶15.0 ng/mL for yeast RNA, respectively. CT DNA could be determined in the presence of 20% (w/w) yeast RNA, and the relative standard deviation of six replicate measurements is 3.18% for 400 ng/mL of CT DNA. Interference from coexisting substances in the determination of DNA was also examined. Real samples were determined with satisfactory results.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' journal of analytical chemistry 362 (1998), S. 537-540 
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel mimetic enzyme immunoassay to determine α-1-fetoprotein (AFP) in solution was developed. Hemin, a horseradish peroxidase substitute, was used as a labelling reagent to catalyze the reaction of p-hydroxyphenylacetic (HPA) and hydrogen peroxide in alkaline media. In the competitive immunoassay, monoclonal anti-AFP antibody was coated on a 96-well plate (polystyrene) and a constant amount of hemin-labelled AFP and a known volume of test solution were added. Non-labelled and hemin-labelled AFP compete for binding to the plate-bound antibody. After the immunoreaction, the immunochemically adsorbed hemin-AFP conjugate moiety was determined by measuring the fluorescence produced in a solution containing HPA and hydrogen peroxide. The calibration graph for AFP was linear over the range 0 ∼ 380 ng/ well with a detection limit of 1.0 ng/well. The method has been applied to determine the AFP in human blood serum with satisfactory results.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-5036
    Keywords: microbial activity ; oil-contamination ; phosphatase activity ; plant growth ; reclamation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Biological properties of soil are not only essential for the maintenance of soil fertility and the sustainability of the plant-soil ecosystems, but also indicators of land reclamation of contaminated or disturbed soils. This experiment involves two plants (barley and field pea) growing in four soils with different hydrocarbon contents. The objective was to study the effect of hydrocarbons on plant growth and microbial activity, and to evaluate the acid phosphatase activity as an indicator of reclamation of hydrocarbon-contaminated soils. Barley root mass decreased with the increase of the hydrocarbon content but field pea roots were not sensitive to the hydrocarbon content in this experiment. The hydrocarbon contamination reduced the plant growth but increased the microbial activity. The acid phosphatase activity was controlled by both plant root production and microbial activity, therefore it was not a good indicator of the reclamation of oil-contaminated soils.
    Type of Medium: Electronic Resource
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