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  • 1
    Electronic Resource
    Electronic Resource
    Secretion of plasminogen activator inhibitor by cultured human epidermal cells (1986)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 115 (1986), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A conditioned medium from cultured human epidermal cells was observed to inhibit the activity of exogenous urokinase. By reverse fibrin autography after SDS polyacrylamide gel electrophoresis, a plasminogen activator inhibitor was detected with a molecular weight of 46000. Using Mr- 33000 [125I]-labelled urokinase we observed the formation of an enzyme-ligand complex. The molecular weight of this complex was 79000. These results indicate that cultured human epidermal cells secrete a plasminogen activator inhibitor (urokinase inhibitor) with a molecular weight of 46000.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1986.tb05708.x
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  • 2
    Electronic Resource
    Electronic Resource
    Plasminogen activator secreted by cultured human melanocytes (1986)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 115 (1986), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A plasminogen activator (PA), Mr 72 000, was detected in conditioned medium from human melanocyte cultures by fibrin autography. The electrophoretic mobility was identical to that of tissue PA produced by Bowes melanoma cells. PA activity in human melanocyte culture medium was inhibited by anti-tissue PA IgG, but not by anti-urokinase IgG. Our results are the first to show that normal human melanocytes in culture secrete tissue plasminogen activator.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1986.tb05719.x
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  • 3
    Electronic Resource
    Electronic Resource
    The value of blood group-specific lectin and endothelial associated antibodies in the diagnosis of vascular proliferations (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 13 (1986), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Blood vascular and lymphatic tumors were evaluated immunohistochemically by studying a spectrum of endothelial associated antigens. (1) UEA-I lectin reacted with the tumor cells of one patient with malignant angioendothelioma in the non-metastatic stage. However, when metastasis occurred, the binding sites of this lectin completely disappeared from the surface of the tumor cells in both original and metastatic lesions, suggesting the loss of blood group H antigen from the tumor cells could be used as an indicator of metastasis in this tumor. (2) Reaction with anti-HLA-A, B, C, intense in normal blood vessels, remained intensely positive in pyogenic granuloma and Kaposi's sarcoma, whereas it did not react with normal lymphatics and lymphangioma. This indicates that anti-HLA-A, B, C is useful in differentiating blood vascular structures from lymphatic structures in both normal and pathological conditions. (3) OKM5 reacted intensely with benign hyperplasius in pyogenic granuloma, while barely reacting with proliferating parts in Kaposi's sarcoma, suggesting the difference in staining patterns can be used to distinguish vascular proliferation or malignancy. (4) Reaction with anti-Type IV collagen and anti-laminin was intense in normal blood vessels, pyogenic granuloma and Kaposi's sarcoma, whereas reaction with these antibodies in normal lymphatics was patchy and irregular in its thickness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1986.tb01084.x
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  • 4
    Electronic Resource
    Electronic Resource
    Variation of the distribution pattern of SH groups and SS linkages in the human epidermis (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of cutaneous pathology 13 (1986), S. 0 
    Details
    ISSN: 1600-0560
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Normal epidermis of various sites of human body was examined by N-(7-dimethyl-amino-4-methyl-3-coumarinyl)maleimidc (DACM) method for SH groups and SS linkages. The horny cells in the palm and sole showed a gradual occurrence of a SH membranous pattern, while those in other sites revealed an abrupt formation of the SH membranous pattern just above the granular layer. In the palm and sole epidermis the number of layers which exhibit membranous pattern of SH-positivity was thicker than that in the other epidermis; the low- through mid-layers of horny cells in the former sites seemed to contain not only SS-positive but also SH-positive substance at cell peripheries. The DACM staining for SS linkages showed the presence of a granular fluorescence in the cytoplasm of the granular cells of the epidermis in the human palm and sole, but not in other sites. The granular cells in human lip displayed a granular fluorescence in the cytoplasm by cither SH or SS DACM staining. All the SH- or SS-positive granular fluorescence was related to keratohyaline granules. These findings indicate that there may be some variations of the distribution pattern of the epidermal cellular proteins with SH and/or SS and a difference in chemical composition of keratohyaline granules between different anatomical sites of human body.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0560.1986.tb01524.x
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  • 5
    Electronic Resource
    Electronic Resource
    Bioactive marine metabolites. VIII. Isolation of an antimicrobial blue pigment from the bryozoanBugula dentata (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 84-84 
    Details
    ISSN: 1420-9071
    Keywords: Bugula dentata ; bryozoa ; antimicrobial ; tetrapyrrole
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antimicrobial blue pigment, which is identical with a tetrapyrrole from a bacterium, was isolated from the bryozoanBugula dentata.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01975907
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  • 6
    Electronic Resource
    Electronic Resource
    Okinonellins A and B, two novel furanosesterterpenes, which inhibit cell division of fertilized starfish eggs, from the marine spongeSpongionella sp (1986)
    Springer
    Cellular and molecular life sciences 42 (1986), S. 1299-1300 
    Details
    ISSN: 1420-9071
    Keywords: Spongionella sp. ; porifera ; cell division inhibitor ; starfish embryos ; furanosesterterpene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two novel furanosesterterpenes, okinonellins A (1) and B (2), have been isolated from the spongeSpongionella sp. Both compounds inhibit cell division of starfish embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01946432
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  • 7
    Electronic Resource
    Electronic Resource
    Synthesis of tris(β-diketonato) technetium(III) and its adsorption behaviour on silica gel (1986)
    Springer
    Journal of radioanalytical and nuclear chemistry 103 (1986), S. 19-26 
    Details
    ISSN: 1588-2780
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Tris (benzoylacetonato) technetium(III) and tris (2-thenoyltrifluoroacetonato) technetium(III) were synthesized by the direct reduction of pertechnetate with dithionite in the presence of excess ligands. The geometrical isomers of the benzoylacetonate were further separated by means of a silica gel column chromatography. In the silica gel system, the adsorption distribution coefficient of the tris (β-diketonato) technetium(III) decreases in the order of Tc(acac)3〉fac-Tc(bzac)3〉mer-Tc(bzac3)〉Tc(tta)3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02165360
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  • 8
    Electronic Resource
    Electronic Resource
    Anodic characteristics of amorphorous palladium-base alloys in sodium chloride solutions (1986)
    Springer
    Journal of applied electrochemistry 16 (1986), S. 565-574 
    Details
    ISSN: 1572-8838
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The anodic characteristics of a variety of amorphous palladium-base alloys were examined with a view to their use for the production of sodium hypochlorite by electrolysis of dilute sodium chloride solutions at ambient temperature. The corrosion resistance of palladium-metalloid alloys was obtained by alloying with platinum group metals and/or valve metals. Among these alloys, rhodium-containing alloys showed high electrocatalytic activities for chlorine evolution. Surface activation treatment was, however, necessary to obtain sufficiently high activities for chlorine evolution at low overpotentials. Surface-activated amorphous alloys possessed considerably higher current efficiency for chlorine evolution in comparison with currently used anodes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01006851
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  • 9
    Electronic Resource
    Electronic Resource
    Isolation of the structural proteins of western equine encephalitis virus by isoelectric focusing (1979)
    Springer
    Archives of virology 60 (1979), S. 299-309 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Western equine encephalitis virus was disrupted with Triton X-100 and subjected to isoelectric focusing in a sucrose or urea gradient. The two envelope proteins, E 1 and E 2 were not well separated in a sucrose gradient, while the E 1 and E 2 proteins were distinguished as two major peaks which focused in a urea gradient at about pH 7.5 and 10, respectively. Isolated E 1 protein refocused at pH 6.5 in a sucrose gradient isoelectric focusing column. When Western equine encephalitis virus was treated with Triton X-100 in 0.01 M phosphate buffer (pH 6), hemagglutinating E 1 protein was solubilized, which isoelectrofocused at pH 6.5. Purified nucleocapsids focused at pH 4 in a sucrose gradient on an isoelectric focusing column. After ribonuclease treatment of the purified nucleocapsid more than 95 per cent of the viral RNA was acid-soluble, and the nucleocapsid protein isoelectrofocused at about pH 4.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01317501
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  • 10
    Electronic Resource
    Electronic Resource
    A novel method of preparing of glass capillary columns: Low-temperature plasma etching and polymerization (1979)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 2 (1979), S. 400-404 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; Capillary, glass ; Low temperature plasma etching ; Polymer film on the inner surface from organosilicone monomer ; Flexibility of this new method discussed ; Discharge chamber described ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A novel method is described for the preparation of stable glass capillary columns (glass open tubular columns), including the etching and formation of a polymer film on the inner glass capillary surfaces. The approach used here is based on low-temperature plasma etching and polymerization. Under the influence of a field of radio frequency discharge, low pressure gases of fluoric compounds, introduced into the glass capillary tube, generate excited fluorine radicals which etch the inner surface. The plasma of organosilicone monomer in the glass capillary yields a uniform polymerized film on the inner surface. The resultant material functions as a good stationary phase for glass capillary gas chromatography (GC2). The inner surfaces treated with such a plasma, can be studied by means of a scanning electron microscope (SEM). The flexibility of this method permits the use of various stationary phases and surface modification.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jhrc.1240020706
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