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  • 1985-1989  (3)
  • 1987  (3)
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  • 1985-1989  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Epitaxial growth of LiNbO3-LiTaO3 thin films on Al2O3 (1987)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 62 (1987), S. 2989-2993 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Epitaxial thin films of LiNbO3 and LiTaO3 were grown on sapphire substrates by the rf-magnetron sputtering method. Structural characterization was made by several spectroscopic measurements: x-ray diffraction, Raman scattering, and optical absorption. We obtained epitaxial thin films with lattice-mismatched strained layers, without misfit defects which are usually created at the interface. Impurity-ion (Nd3+ and Cr3+) doped films were also grown; these films had larger refractive indices than the pure films. Furthermore, the heteroepitaxial thin films of up to 10 alternating LiNbO3/LiTaO3 layers were successfully grown, and their periodic structures were studied by optical second-harmonic generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.339385
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    An immobilized cell culture system for berberine production by Thalictrum minus cells (1987)
    Springer
    Plant cell reports 6 (1987), S. 185-186 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thalictrum minus cells immobilized in calcium-alginate beads were cultured in a newly devised bioreactor for the purpose of producing berberine. This system could prevent cells from leaking out of the beads, allowing them to release most of the berberine synthesized into the liquid medium. Furthermore, the biosynthetic capability of the immobilized cells was found to be comparable to that of freely suspended cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00268474
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Antigenicity of Japanese encephalitis virus envelope glycoprotein V 3 (E) and its cyanogen bromide cleaved fragments examined by monoclonal antibodies and Western blotting (1987)
    Springer
    Archives of virology 96 (1987), S. 97-107 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Purified Japanese encephalitis (JE) virus was solubilized under reducing condition by using 2-merceptoethanol (2 ME) or nonreducing condition without 2 ME and its structural proteins were separated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS-PAGE), followed by the Western blotting using polyclonal and monoclonal antibodies against JE. The mobilities and reactivities against polyclonal anti-serum of V 3 (E) and V 2 (C) were reduced when virion was disrupted under reducing condition. The 54 K band corresponding to V 3 was treated with cyanogen bromide (CNBr) and analyzed by the second SDS-PAGE and Western blotting. By Coomassie Blue staining multiple bands of molecular weight ranging from 54 K to 8 K daltons were revealed for CNBr-treated V 3. For the specimens disrupted under reducing condition, uncleaved 54 K and cleaved 8 K, 14 K, 45 K, and 48 K bands were reactive by one of the JE and Murray Valley encephalitis (MVE) crossreactive monoclonal antibodies (NARMA 16), while other monoclones did not show any reactivity. The uncleaved V 3 prepared under nonreducing condition was reactive with several monoclones to almost similar levels. After CNBr treatment, the antigenic epitope(s) for a flavivirus-common monoclone (NARMA 24) and those for NARMA 16 appeared to locate on different fragments, while the epitopes for other monoclones lost their antigenicities. These results indicate the importance of disulfide bond and highly organized structure to maintain some of the epitopes on V 3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310993
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    Paper (German National Licenses)
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