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  • 1
    Electronic Resource
    Electronic Resource
    Allergens from birch pollen and pollen of the European chestnut share common epitopes (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 23 (1993), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Type I allergy to pollen of the European chestnut (Castanea sativa) represents a major cause of pollinosis in (sub) Mediterranean areas. Using sera from 14 patients with established allergy to pollen of the European chestnut, 13/14 sera (92%) showed IgE-binding to a 22 kD protein, 2/14(14%) displayed additional binding to a 14 kD protein and 1/14 (7%) bound only to the 14 kD protein of European chestnut pollen extract. Two monoclonal mouse antibodies, BIP 1 and BIP 4, directed against different epitopes of Bet v I (the major birch pollen allergen), and a rabbit antibody to recombinant birch profilin (rBet v II) were used to characterize the proteins of the European chestnut pollen. The recombinant birch pollen allergens, r Bet v I and r Bet v II (profilin) were employed to show common allergenic structures on proteins from both birch and European chestnut pollen by IgE-inhibition experiments. Despite the fact that the 22 kD protein displayed a higher molecular weight in comparison to the 17 kD major birch pollen allergen, Bet v I, we could demonstrate reactivity of both monoclonal antibodies, BIP 1 and BIP 4, with this protein. A complete inhibiton of IgE-binding to this 22 kD protein was shown by pre-incubating sera with purified recombinant Set r I. In addition, the 14kD protein could be identified by IgE-inhibition studies with recombinant Bet v II and by using a rabbit anti-profilin antibody as the profilin from pollen of the European chestnut.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1993.tb00363.x
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  • 2
    Electronic Resource
    Electronic Resource
    Human monocytes and keratinocytes in culture ingest hydroxyethylstarch (1993)
    Springer
    Archives of dermatological research 285 (1993), S. 144-150 
    Details
    ISSN: 1432-069X
    Keywords: Hydroxyethylstarch ; Phagocytosis ; Keratinocytes ; Monocytes ; Flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Severe itching for unknown reasons has been reported after administration of hydroxyethylstarch (HES) in haemodilution therapy of humans. In skin sections from patients who had received HES for otological reasons HES deposition was found by immunohistochemical methods in keratinocytes, epidermal dendritic cells, endothelial cells and macrophages. The aim of the present investigation was to study the interaction of cultured human monocytes and keratinocytes, normal (HNK) and malignant (KB, A431), with FITC-conjugated HES (200/0.5). Peripheral blood monocytes from healthy donors were isolated by Ficoll-Hypaque gradient centrifugation and adherence to plastic surfaces. Cells were incubated with HES-FITC (1 mg/ml) for 10, 20, 30, 60 and 120 min at 37
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01112917
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  • 3
    Electronic Resource
    Electronic Resource
    Hydroxyethylstarch deposits in human skin — a model for pruritus? (1993)
    Springer
    Archives of dermatological research 285 (1993), S. 13-19 
    Details
    ISSN: 1432-069X
    Keywords: Hydroxyethylstarch ; Tissue storage ; Macrophages ; Immunohistochemistry ; Itching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Severe itching for unknown reasons has been reported after administration of hydroxyethylstarch (HES) in haemodilution therapy of humans. After HES treatment, vacuoles in cells of various organs in humans have been shown, predominantly affecting the mononuclear phagocyte system. These vacuoles present indirect evidence for phagocytosis of HES particles. Since phagocytosis is also known to occur in the skin, this organ might represent a target for HES deposition, resulting in subsequent release of mediators responsible for the observed itching. The aim of the present investigation was to study skin biopsies of patients, who had received HES and suffered subsequently from itch. Skin sections were investigated for morphological impairment by means of light and electron microscopy, immunohistochemistry and immunoelectron microscopy using a polyclonal anti-HES antiserum. Storage of HES was demonstrated in the skin of all patients, mainly in dermal macrophages, endothelial cells of blood and lymph vessels, some perineural cells and endoneural macrophages of larger nerve fascicles, some keratinocytes and Langerhans cells. Treatment with antihistaminic agents proved ineffective in these patients; this fits with the observation that morphological signs of histamine release from mast cells were absent. These findings indicate that other mediators from HES-affected cells must be responsible for the development of the itching. Thus, investigation of HES storage may be a useful contribution to the elucidation of release of itch mediators and induction of pruritus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00370817
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  • 4
    Electronic Resource
    Electronic Resource
    The expression and cellular distribution of adhesion molecules CD2/LFA-3 and ICAM-1/LFA-1 on mononuclear cells in squamous cell carcinoma of the head and neck (1993)
    Springer
    European archives of oto-rhino-laryngology and head & neck 250 (1993), S. 168-172 
    Details
    ISSN: 1434-4726
    Keywords: Mononuclear cells ; Adhesion molecules ; Cellular distribution ; Squamous cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expressions and cellular distributions of two pairs of adhesion molecules CD2/LFA-3 (leukocyte function-associated antigen-3) and LFA-1/ICAM-1 (intercellular adhesion molecule-1) were examined in inflammatory cellular infiltrates of advanced squamous cell carcinomas of the head and neck by immunohistochemical techniques including double-staining methods. Thirteen patients were investigated using the following monoclonal antibodies (mAbs): CD2, LFA-3 (CD58), ICAM-1 (CD54), LFA-1 (CD11a), the alpha/beta and gamma/delta T-cell receptor, pan T cells and broadly distributed monocyte/macrophage (m/mø) [Fc gamma RII (CD32), 25F9, RM3/1]. LFA-3 staining was observed on a high number of cells (968 ± 112 cells/mm2), correlating to the number of Fe gamma RII (CD32; P 〈 0.01), 25F9 (P 〈 0.05) and RM3/1 (P 〈 0.05) positive m/mø. Its ligand CD2 was found on 365 ± 126 cells/mm2, representing about 50% of CD3+ cells (730 ± 286 cells/mm2). CD2 positivity correlated to CD3 and CD8 (P 〈 0.01) but not to CD4+ T cells. LFA-1 and ICAM-1 were expressed on lymphocytes as well as on m/mø. ICAM-1+ cells (902 ± 205 cells/mm2) correlated to CD3+, CD8+ and RM3/1+ cells (P 〈 0.01). LFA-1 positivity (803 ± 255 cells/mm2) showed correlations to nearly all investigated antigens, as well as to CD4+ T cells (P 〈 0.05). These results show that different m/mø subsets display distinct patterns of adhesion molecule expressions suggesting different pathways of regulation. The CD3+ lymphocyte population revealed a lack of CD2 expression that was more pronounced in the CD4+ subset and indicated impaired lmyphocyte function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171705
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