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  • 2005-2009  (2)
  • 1980-1984  (2)
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  • 1
    Electronic Resource
    Electronic Resource
    'Top Down' Approaches for the Study of Single-Cells: Micro-Engineering and Electrical Phenotype (2006)
    s.l. ; Stafa-Zurich, Switzerland
    Advances in science and technology Vol. 53 (Oct. 2006), p. 97-106 
    Details
    ISSN: 1662-0356
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Natural Sciences in General , Technology
    Notes: Single-cell analysis is a very important field of research and is currently at the frontier ofphysical and biological sciences. Understanding how the phenotype of a single-cell arises from itsgenotype is a complex topic. Currently, the prevailing paradigm to analyze cellular functions is thestudy of biochemical interactions using fluorescence based imaging systems. However, theelimination of the labelling process is highly desirable to improve the accuracy of the analysis.Living cells are electromagnetic units; in as much they use electric mechanisms to control andregulate dynamic processes involved in inter alia signal transduction, metabolism, proliferation anddifferentiation. Recent developments in micro- and nanofabrication technologies are offering greatopportunities for the analysis of single cells; the combination of micro fluidic environments, nanoelectrodes/wires and ultra wide band electromagnetic engineering will soon make possible theinvestigation of local (submicrometer scale) dynamic processes integrating several events atdifferent time scales. In the paper, we present recent approaches which aim at investigating singlecellswith the help of MEMS and NEMS (Micro and Nano Electro Mechanical Systems) and ultrawide band (DC-THz) electromagnetic characterization techniques
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/01/42/transtech_doi~10.4028%252Fwww.scientific.net%252FAST.53.97.pdf
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A new SiC polytype, 45 Rb (1982)
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 38 (1982), S. 477-482 
    Details
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The stacking sequence of a new SiC polytype 45Rb, which was synthesized at room temperature is (433212)3. This implies that the Zhdanov symbol 1 in the stacking sequence, except for the 2H polytype, might be a special characteristic of the structure of SiC grown at low temperatures. If this is true, the recently reported 5H polytype SiC found in limestone, dolomite and alluvial deposit, which can be deduced as (41) stacking, may be authigenic and might have grown at a low temperature. This idea may suggest the general distribution of SiC in limestone and in other low- temperature deposits. Unmetamorphosed limestones of about 20 localities were examined, and SiC was found in seven of those localities, although the 45Rb and 5H polytypes have not been found yet in these samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S056773948200103X
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Human mesenchymal stem cells successfully improve skin-substitute wound healing (2005)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 153 (2005), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  Large or deteriorated skin defects are sometimes life threatening. There is increasing evidence that adult stem cells are useful for tissue regeneration. Human mesenchymal stem cells (hMSCs) are self-renewing and are potent in differentiating into multiple cells and tissues.Objectives  To investigate the effects of hMSCs in cutaneous wound healing.Methods  Wound healing was studied in an hMSC-populated porcine skin substitute, using a nude rat model to minimize immune reactions. Full-thickness skin and soft tissue defects of 1·5 × 1·5 cm in size, including the panniculus carnosus, were excised and covered with hMSCs and basic fibroblast growth factor (bFGF)-soaked skin substitutes and an evaluation was made of wound size, histology and protein expression at 3, 7 and 42 days after injury.Results  The wound size was significantly smaller in the hMSC-treated groups (P 〈 0·01) and any dose of bFGF (1, 10, 100 μg) enhanced the healing (P 〈 0·01). The re-epithelialization markers integrin α3 and skin-derived antileucoproteinase were remarkably increased with the presence of bFGF in a dose-dependent manner, while the mesenchymal cell surface markers CD29 and CD44 were downregulated in a time-dependent manner. Human pancytokeratin, which does not cross-react with rat antigens, was observed by Western blotting at 38 kDa and 42 kDa from the hMSC-treated tissues on day 7. The expression levels were elevated by 10 μg bFGF (P 〈 0·01). The immunohistochemical expression of human pancytokeratin was only observed in the hMSC-treated groups.Conclusions  These data suggest that hMSCs together with bFGF in a skin defect model accelerate cutaneous wound healing as the hMSCs transdifferentiate into the epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2005.06554.x
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Simultaneous measurement of pyridine nucleotide fluorescence and field potentials from the olfactory cortical slice of the guinea-pig (1981)
    Springer
    Cellular and molecular life sciences 37 (1981), S. 206-208 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new probe (tip diameter=700 μm) consisting of a micro-light guide and a silver wire electrode was constructed to measure pyridine nucleotide fluorescence and field potentials from nerve tissues. Good kinetic correlations were found between the changes in field potentials and tissue pyridine nucleotide fluorescence caused by stoppage or by resumption of superfusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01963236
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    Paper (German National Licenses)
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