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  • 1
    Electronic Resource
    Electronic Resource
    Contents of aprotinin, heparin and histamine in mast cells from bovine liver capsule (1986)
    Springer
    Inflammation research 18 (1986), S. 325-328 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Mast cells were isolated from the bovine liver capsule by treatment with collagenase. The extract from the mast cells contained aprotinin. The concentrations of aprotinin, heparin and histamine in these mast cells were (196±42) pg/cell, (1.6±0.2)×10−4 IU/cell and (32±3) pg/cell (mean±S.E.M. of 8–11 experiments), respectively, determined by an enzyme immunoassay, spectrophotometrically and fluorometrically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964992
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  • 2
    Electronic Resource
    Electronic Resource
    Immunogenetic heterogeneity in Type 1 (insulin-dependent) diabetes among Japanese — HLA antigens and organ-specific autoantibodies (1989)
    Springer
    Diabetologia 32 (1989), S. 34-39 
    Details
    ISSN: 1432-0428
    Keywords: Type 1 (insulin-dependent) diabetes ; HLA ; auto-antibodies ; islet cell antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary HLA phenotypes and haplotypes in relation to organ-specific autoantibody responses were studied in 82 Japanese patients with Type 1 (insulin-dependent) diabetes. HLA-DRw9 antigen and HLA phenotype of DRw9/X (X: not DR4) were increased in patients with organ-specific autoantibodies other than islet cell antibody (CP〈0.02, RR=4.02 and p〈0.05, RR=2.30, respectively); whereas HLA-DR4 antigen and HLA phenotype of DR4/X (X: not DRw9) were increased in those without the autoantibodies (CP〈0.001, RR=3.95 and p〈0.01, RR=2.46, respectively). HLA haplotype of Bw61-DRw9 was increased in patients with the autoantibodies (p〈0.005, RR=4.94), and HLA haplotype of Bw54-DR4 was increased in those without the autoantibodies (p〈0.001, RR=5.52). The relative risk of HLA-DR4/DRw9 was the highest among all HLA-DR phenotypes or genotypes in patients either with or without the autoantibodies. No association was, however, found between the incidence of islet cell antibody and HLA-DR phenotypes. These findings suggest that Type 1 diabetes among Japanese is immunogenetically heterogeneous as is Type 1 diabetes among Caucasians; and the differences in HLA-association of Type 1 diabetes among ethnic groups might give a clue to understanding of a role of HLA-antigens in the development of Type 1 diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265401
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  • 3
    Electronic Resource
    Electronic Resource
    Intrahepatic biliary calculi: correlation of unusual MR findings with pathologic findings (2000)
    Springer
    Abdominal imaging 25 (2000), S. 266-268 
    Details
    ISSN: 1432-0509
    Keywords: Key words: Liver—Biliary—Calculi—Magnetic resonance imaging.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case of intrahepatic biliary calculi. A localized dilated intrahepatic duct of the left lateral segment of the liver was filled with material that showed marked hyperintensity on T1- and T2-weighted magnetic resonance (MR) images. These MR findings are unusual for intrahepatic stones. Pathologically, the stones were muddy bilirubin calculi, the chemical and physical characteristics of which are surmised to have been the cause of the unusual MR findings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002610000030
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  • 4
    Electronic Resource
    Electronic Resource
    A new cultivation method of fungi or mycelia (1988)
    Springer
    Bioprocess and biosystems engineering 3 (1988), S. 63-68 
    Details
    ISSN: 1432-0797
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The conventional penicillin submerged culture has several drawbacks: filamentous fungi solidify into pellets or become pulpy, which has so far had bad effects on the separation of penicillin from the broth, on the monitoring and controlling of the culture system as well as on the productivity of penicillin. By using polyurethane foams as a carrier for the fungi Penicillium chrysogenum, the authors have studied a novel cultivation method for this microorganism. The following results were obtained: 1. The effect of urethane foams on the growth characteristics of Penicillium chrysogenum was studied. It was found that the microorganism crept into the carrier, then formed a biofilm of 0.45 mm in thickness on the surface layer of the carrier. 2. The use of urethane foams had the effect of improving not only the mass transfer rates of various nutrients, but also that of oxygen in the culture system. Six times as much penicillin could be produced as is obtainable by traditional cultivation methods. 3. An operational strategy for the addition of polyurethane Toams to the culture medium could be developed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00369329
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  • 5
    Electronic Resource
    Electronic Resource
    Prolonged ventilation of the premature newborn rabbit after treatment with natural or apoprotein-based artificial surfactant (1988)
    Springer
    European journal of pediatrics 147 (1988), S. 168-173 
    Details
    ISSN: 1432-1076
    Keywords: Surfactant ; Hydrophobic apoproteins ; Premature newborn rabbits ; Lung compliance ; Image analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Premature rabbit neonates (gestational age 27 days) were treated at birth with natural surfactant purified from chloroform extracts of porcine lung lipids either by acetone precipitation (Surfactant CK, n=10) or liquid gel chromatography (Curosurf, n=22). Another group of animals received artificial surfactant “reconstituted” from isolated low molecular weight (≤15 K) apoproteins and synthetic dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) (Aposurf, n=10). The phospholipid concentrations of the preparations were adjusted to provide the same individual dose of DPPC for each group of treated animals (3 or 4 mg). In comparison with untreated controls from the same litters, there was a 4–7-fold enhancement of lungthorax compliance in all groups of surfactant-treated animals during a 3-h period of artificial ventilation. The average initial (20 min) compliance value was lower in the Aposurf-treated group than in animals receiving natural surfactant preparations, but the difference between the groups gradually diminished and was no longer statistically significant during the 2nd and 3rd h of artificial ventilation. Judged from the fall in tidal volume during ventilation with a short expiration phase (0.17 instead of 0.75s), the apoprotein-based artificial surfactant was also less effective in stabilizing the lungs. A similar conclusion could be drawn from data on alveolar expansion in histological sections, evaluated by automated image analysis. Alveolar volume density was improved only moderately in the Aposurf-treated group (0.24 vs. 0.14; P=0.05), whereas the expected, prominent increase in this parameter was observed in both groups of natural surfactant-treated animals (0.48–0.62 vs. 0.14; P〈0.001). We conclude that a physiologically active artificial surfactant can be prepared from the smaller (≤15 K) apoproteins, DPPC and DPPG; the in vivo effects of this preparation were clearly beneficial, yet inferior to those obtained with the same dose of natural surfactant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442216
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  • 6
    Electronic Resource
    Electronic Resource
    Enhancement of human fibroblast growth and other dermatological effects induced by cell extract from heat-shocked blue green alga (Cyanobacteria) (2004)
    Oxford, UK : Blackwell Science Ltd
    International journal of cosmetic science 26 (2004), S. 0 
    Details
    ISSN: 1468-2494
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: It has been known that high amounts of ‘heat shock proteins (HSPs)’ are produced under heat and other environmental stresses in cells. Recently, it was reported that HSP could also be accumulated in some dermal cells to protect the cellular proteins from damage caused by ultraviolet radiation. Here, the authors have focused on thermophilic cyanobacteria and the heat shock treatment. Thermophilic cyanobacteria are known to thrive under extreme environmental conditions, and produce distinctively high amounts of HSP when exposed to higher temperatures. We tested cell extract from heat-shocked cyanobacterial cells on a number of skin-related biological activities and found it significantly effective. The cyanobacterial cells, originally collected from a spa as hot as 55 °C, were cultivated in the laboratory at 50 °C and broken by a freeze-fracture method. The extract thus obtained had a superoxide dismutase (SOD)-like activity and inhibited lipid peroxide formation. We found that the extract obtained from the heat-shocked (at 63 °C) cells showed a much stronger SOD-like activity, while the inhibitory effect on lipid peroxide formation remained almost the same. Furthermore, the SOD-like activity could withstand heat up to 70 °C for as long as 1 h. Most remarkably, supplementing this heat-shocked extract strongly promoted the proliferation of human dermal fibroblast culture. Extracts from the cells grown at a regular temperature (50 °C) did not promote the proliferation of the fibroblasts. The heat-shocked extract was fractionated by ultrafiltration for further analysis. A fraction (molecular weight: 10 000–30 000) has shown the strongest activity so far. These results indicate that the heat-shocked cyanobacterial extract with its increased amounts of HSPs is a promising potent ingredient for anti-aging cosmetic materials.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1467-2494.2004.00224_5.x
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  • 7
    Electronic Resource
    Electronic Resource
    Sensory Modeling of Coffee with a Fuzzy Neural Network (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 67 (2002), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Models were constructed to predict sensory evaluation scores from the blending ratio of coffee beans. Twenty-two blended coffees were prepared from 3 representative beans and were evaluated with respect to 10 sensory attributes by 5 coffee cup-tasters and by models constructed using the response surface method (RSM), multiple regression analysis (MRA), and a fuzzy neural network (FNN). The RSM and MRA models showed good correlations for some sensory attributes, but lacked sufficient overall accuracy. The FNN model exhibited high correlations for all attributes, clearly demonstrated the relationships between blending ratio and flavor characteristics, and was accurate enough for practical use. FNN, thus, constitutes a powerful tool for accelerating product development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2002.tb11411.x
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  • 8
    Electronic Resource
    Electronic Resource
    Simple and inexpensive interferometry of shock wave developments from pulsed high-current discharge (2000)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 71 (2000), S. 1548-1551 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Interferometer measurements are extremely informative in shock wave studies allowing direct evaluations of the gas density. The primary goal of the work presented is to build a laser interferometer that meets the requirements of the highest possible simplicity, economy, convenience, and ease of construction. In our experiments, we used a low average power (10 mW) He–Ne laser without complication, expense, and environmental section. The He–Ne laser interferometer with the Michelson arrangement was used to measure the line-averaged gas densities of shock waves. Temporal and spatial measurements of the density were performed for shock wave developments from high-current pulse discharges. The shock wave propagates in the radial direction of the discharge channel with supersonic speed. The shock velocity, however, decreased from 2–0.5 km/s with the distance of the shock propagation. The width of the high density region, that is in the vicinity of the shock front was compressed from 3.5 to 1.8 mm according to the time variation of the discharge current. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1150492
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  • 9
    Electronic Resource
    Electronic Resource
    Formation of electronically excited nitric oxide and energy partitioning in the 193-nm photolysis of the nitric oxide dimer (1985)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 89 (1985), S. 2725-2727 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100259a004
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  • 10
    Electronic Resource
    Electronic Resource
    Excited-state absorption spectroscopy of ortho-metalated iridium(III) complexes (1987)
    s.l. : American Chemical Society
    The @journal of physical chemistry 〈Washington, DC〉 91 (1987), S. 6104-6106 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/j100308a012
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