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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of a new Japanese cedar pollen allergen homologous to plant isoflavone reductase family (2002)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 32 (2002), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Japanese cedar (Cryptomeria japonica) pollen is a major cause of seasonal pollinosis, and more than 10% of Japanese people suffer from this allergic disorder. However, only two major pollen allergens, Cry j 1 and Cry j 2, have been identified and exclusively characterized.Objective The aim of this study was to explore and identify important Japanese cedar pollen allergens other than Cry j 1 or Cry j 2.Methods C. japonica cDNA library was immunoscreened by rabbit antiserum raised against a partially purified cedar pollen allergen fraction. An isolated cDNA clone was inserted into a glutathione S-transferase (GST)-tagged Escherichia coli expression vector to obtain recombinant GST fusion protein. Non-fusion recombinant protein was purified by glutathione Sepharose affinity chromatography in conjunction with factor Xa cleavage of the GST moiety. IgE-binding ability of the recombinant protein was then evaluated by western blot analysis and enzyme-linked immunosorbent assay (ELISA).Results The cDNA encodes 306 amino acids with significant sequence similarity to those of plant isoflavone reductase-like proteins, which include a recently identified birch pollen allergen Bet v 5. Western blot analysis demonstrated that recombinant protein was recognized by cedar pollinosis patient IgE. In contrast to Bet v 5 being reported as a minor allergen, the recombinant protein exhibited 76% IgE binding frequency (19/25) against pollinosis patients.Conclusion Here we identified the third member of Japanese cedar pollen allergen homologous to isoflavone reductase. Its high IgE-binding frequency implicates that the isoflavone reductase homologue might be an additional major pollen allergen in C. japonica.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2002.01405.x
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  • 2
    Electronic Resource
    Electronic Resource
    Association between interleukin-18 gene polymorphism 105A/C and asthma (2003)
    Oxford, UK : Blackwell Science Ltd
    Clinical & experimental allergy 33 (2003), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background IL-18 has been shown to exert anti-allergic or allergy-promoting activities, but the existence of genetic polymorphisms in the coding regions of IL-18 gene has not been demonstrated.Objective The aim of this study was to investigate whether polymorphism is present in the coding regions of the IL-18 gene and, if so, to further analyse the association between polymorphism and asthma in a case–control study.Methods We screened the coding regions of the IL-18 gene for polymorphisms by using PCRsingle-stranded conformation polymorphism and direct sequencing of PCR products, followed by analysis of the association between polymorphism and asthma.Results We identified one polymorphism (105A/C) in the coding regions. The frequency of the 105A allele was significantly higher in asthmatic patients than in controls (P〈0.01; odds ratio (OR)=1.83 (1.37–2.26)). Significant linkage disequilibrium was observed between the 105A/C and −137G/C polymorphisms in the 5′ flanking region of the IL-18 gene (D=0.58, P〈0.0001). However, in asthmatic patients the 105A allele was not associated with either total serum IgE or IL-18 levels.Conclusion The 105A/C polymorphism of the IL-18 gene may be associated with the pathogenesis of asthma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2003.01739.x
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  • 3
    Electronic Resource
    Electronic Resource
    Shear modulus and thermal properties of gutta percha for root canal filling (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 31 (2004), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  The purpose of this investigation was to investigate the rheological properties of four commercially available gutta perchas for root canal filling. The relaxation modulus [Gr(0): instantaneous shear modulus] and specific volume of their materials were examined. In addition, the quantity of heat was also studied by differential scanning calorimeter. In a lower temperature range than the first-order transition temperature (melting point), the Gr(0) values of each material were almost identical. A marked decrease of Gr(0) was observed at the melting point, and the range of the first-order transition temperature at heating was from 42·0 to 60·0 °C. At higher temperatures than the first-order transition temperature of each material, a considerable difference in Gr(0) values was observed. The transition temperatures obtained by the results of the Gr(0), specific volume and quantity of heat agreed with one another. A marked specific volume change was observed at the first-order transition temperature. The technique using melted gutta percha may not be favourable compared with the conventional lateral condensation technique because melted gutta percha undergoes a large amount of shrinkage during setting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2842.2004.01349.x
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  • 4
    Electronic Resource
    Electronic Resource
    Masticatory movement in two cases with unusual alignment of the maxillary canine (2000)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 27 (2000), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Masticatory function was analysed before and after orthodontic treatment in two cases where tooth alignment remained unusual after treatment. The Sirognathograph Analyzing System® was used to analyse the masticatory function. In both cases, the right maxillary first premolar was located where the maxillary canine is normally positioned. The results of orthodontic treatment were satisfactory both morphologically and aesthetically, and masticatory function was greatly improved. However, some problems remained in the mandibular movement due to the abnormal contact between the mandibular teeth and the maxillary first premolar. These findings support the need for examination of masticatory function when treating patients with an unusual tooth alignment even if the results of orthodontic treatment are both morphologically and aesthetically successful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2000.00565.x
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  • 5
    Electronic Resource
    Electronic Resource
    Bonding abutments to cast metal post/cores: comparison of pre-treatment effects (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 30 (2003), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  Bond strengths were evaluated for (1) metal primer systems when the metal was contaminated by a dentin conditioner and (2) a dentin adhesive system when dentin was contaminated by metal primers. Disc specimens were cast in a silver–palladium–copper–gold (Ag–Pd–Cu–Au) alloy and dentin specimens were prepared by grinding the labial surface of bovine teeth. Specimens were treated with (1) metal primer alone, (2) dentin conditioner alone, (3) metal primer followed by dentin conditioner and (4) dentin conditioner followed by metal primer. A resin cement was poured into a mould over a restricted bonding area and allowed to set. Metal specimens were shear stressed to failure after thermocycling (4–60 °C; 20 000 cycles). Dentin specimens were stressed in the same manner after 24 h of immersion in 37 °C water. The results were compiled and analysed by anova. Data for dentin specimens treated with dentin conditioner only or with the combination of dentin conditioner and metal primer were not significantly different, statistically. Post-thermocycled groups indicated that bond strengths to the alloy significantly decreased (P 〈 0·05) when the primed metal surface was contaminated with dentin conditioner regardless of the timing of its application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2003.01016.x
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  • 6
    Electronic Resource
    Electronic Resource
    Epidermolysis bullosa acquisita sera react with distinct epitopes on the NC1 and NC2 domains of type VII collagen: study using immunoblotting of domain-specific recombinant proteins and postembedding immunoelectron microscopy (2004)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 150 (2004), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  The sera of epidermolysis bullosa acquisita (EBA) react with type VII collagen, a major component of anchoring fibrils, in which the major epitopes have been considered to be present in the N-terminal noncollagenous (NC) 1 domain.Objectives  To determine whether there are also epitopes in the C-terminal NC2 domain, and to determine their ultrastructural localization.Methods  Immunoblotting using recombinant proteins of the NC1 and NC2 domains of type VII collagen, and postembedding immunoelectron microscopy.Results  Twenty of 28 EBA sera tested reacted with the NC1 domain and eight sera reacted with the NC2 domain. The sera that reacted with the NC1 domain showed immunoreactivity within the lamina densa and the sera that reacted with the NC2 domain showed immunoreactivity in the dermis 300–360 nm below the lamina densa.Conclusions  This study clearly identified the presence of epitopes in the NC2 domain, and showed that the epitope in the NC1 domain is present in the lamina densa and that the epitope in the NC2 domain is in the dermis below the lamina densa.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.2004.05933.x
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  • 7
    Electronic Resource
    Electronic Resource
    Administration of anti-interleukin 18 antibody fails to inhibit development of dermatitis in atopic dermatitis-model mice NC/Nga (2003)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 149 (2003), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Summary Background Interleukin (IL)-18 has been shown to activate basophils to produce histamine and IL-4 and to induce naive T cells to differentiate into T-helper (Th) 2 cells. However, when expressed together with IL-12, IL-18 induces Th1 cell development and inhibits IgE synthesis. Previously we reported that serum IL-18 levels were elevated in the sera from atopic dermatitis-model mice NC/Nga, prior to the onset and during the development of dermatitis. Objectives We studied whether neutralization of IL-18 activity might affect dermatitis in NC/Nga mice, to investigate the role of IL-18 on dermatitis. Methods NC/Nga mice were given weekly anti-IL-18 antibody starting at 5 weeks of age to 13 weeks and development of dermatitis, scratching behaviour and serum IgE concentrations were evaluated. Results Continuous injections of anti-IL-18 antibody failed to inhibit the onset and development of dermatitis and IgE elevation. The treatment, rather, tended to lead to an exacerbation of dermatitis and scratching behaviour. In addition, the administration of anti-IL-18 antibody did not ameliorate the responsiveness of lymphocytes to IL-4, which was previously demonstrated as an immunological abnormality in the mouse. Conclusion This study demonstrates that, at least in NC/Nga mice, IL-18, although excessively expressed before the onset of dermatitis, shows antiallergic actions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2003.05406.x
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  • 8
    Electronic Resource
    Electronic Resource
    Compound heterozygous group A xeroderma pigmentosum patient with a novel mutation and an inherited reciprocal translocation (2000)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 143 (2000), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The severity of neurological abnormalities in Japanese group A xeroderma pigmentosum (XP-A) patients correlates with the sites of non-sense mutation in the XP-A gene. We describe a patient who presented with a more severe photosensitivity and neurological abnormality than those in typical Japanese XP-A patients with a splicing mutation in intron 3. The patient was compound heterozygous for the splicing mutation in intron 3, which resulted in formation of a non-sense codon in exon 4, and a novel non-sense mutation at codon 208 in exon 5, a C to T transition creating a stop codon TAG. Although the combination of these mutations might have been thought to cause only mild neurological signs, the longer truncated XP-A proteins than those of typical XP-A patients may have resulted in severe neurological symptoms. This phenomenon may be explained by a translocation of chromosome (1;10)(q25·3;q22·3) inherited from his father.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2000.03611.x
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  • 9
    Electronic Resource
    Electronic Resource
    Consideration on an undulator magnetic structure for polarization control (2002)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 73 (2002), S. 1724-1727 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A magnetic structure composed of electromagnets and permanent magnets is proposed as an undulator to control the polarization state of synchrotron radiation. The horizontal field is produced by the permanent magnets and tuned by moving them along the undulator axis, while the vertical field is produced by the electromagnets and tuned by changing the coil current. The proposed device has two important degrees of freedom. One is that of the periodic length of the electromagnets and the other is that of the relative phase between the vertical and horizontal fields. Thanks to them, the proposed device works as various types of undulator to realize polarization control. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1453503
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  • 10
    Electronic Resource
    Electronic Resource
    Green emission from B2N2CO thin films doped with Tb (2002)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 81 (2002), S. 34-36 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Visible light emission has been obtained at room temperature by photoluminescence (PL) and cathodoluminescence (CL) from Tb-doped B2N2CO thin films prepared by radio-frequency magnetron sputtering. The films were deposited by sputtering TbBn (n=4, 6, 12, 25, and 50) targets in an atmosphere of Ar and N2 gas mixture. The films are uniformly composed of very small particles with particle size of about 20–40 nm, which consist of turbostratic BN phase and amorphous phase. The characteristic PL peaks are observed in the spectral range from 300 to 800 nm, and correspond to the two groups of transitions of Tb3+ ions: 5D3→7FJ (J=6, 5, 3) and 5D4→7FJ (J=6, 5, 4, 3). The strongest PL peak at ∼547.2 nm is about four times greater than the other signals in the investigated range, indicating that the films have strong green light emission. CL intensity is very weakly dependent on the temperature. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1490143
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