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  • 1
    Electronic Resource
    Electronic Resource
    Molecular cytogenetic analysis of a durum wheat ×Thinopyrum distichum hybrid used as a new source of resistance to Fusarium head blight in the greenhouse (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 120 (2001), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Fusarium head blight (FHB, scab), caused by Fusarium graminearum Schwabe, is a serious and damaging disease of wheat. Although some hexaploid wheat lines express a good level of resistance to FHB, the resistance available in hexaploid wheat has not yet been transferred to durum wheat. A germplasm collection of Triticum durum× alien hybrid lines was tested as a potential source of resistance to FHB under controlled conditions. Their FHB reaction was evaluated in three tests against conidial suspensions of three strains of F. graminearum at the flowering stage. Two T. durum×Thinopyrum distichum hybrid lines, ‘AFR4’ and ‘AFR5′, expressed a significantly higher level of resistance to the spread of FHB than other durum-alien hybrid lines and a resistant common wheat line ‘Nyu-Bay’. Genomic in situ hybridization using total genomic DNA from alien grass species demonstrated that ‘AFR5’ had 13 or 14 alien genome chromosomes plus 27 or 28 wheat chromosomes, while ‘AFR4’ had 22 alien genome and 28 wheat chromosomes. All of the alien chromosomes present in these two lines belonged to the J genome. ‘AFR5’ is likely to be more useful as a source of FHB resistance than ‘AFR4’ because of its relatively normal meiotic behaviour, high fertility and fewer number of alien chromosomes. ‘AFR5’ shows good potential as a source for transferring FHB resistance gene into wheat. The development of T. durum addition lines carrying resistance genes from ‘AFR5’ is underway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2001.00630.x
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  • 2
    Electronic Resource
    Electronic Resource
    Improving male fertility restoration of common wheat for Triticum timopheevii cytoplasm (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The fertile pure line R3-37 of common wheat with cytoplasm of Triticum timopheevii Zhuk. is an R-line (restorer) that can restore the male fertility of A-lines (male sterile lines) with T. timopheevii cytoplasm. In breeding hybrid wheat, the hybrid of the cross R3-37/ Baimian3 was found to be completely male sterile, indicating that Baimian3 has some genes that are epistatic to the Rf genes in R3-37. In order to elucidate the essence of this phenomenon, the male fertilities of the hybrids of 27 crosses including R3-37 and/or Baimian3 were studied. The results show that inheritance of male fertility of the hybrid R3-37/Baimian3 involves interactions among Rf alleles, male fertility-inhibiting genes and genetic background. Although more than 70 different kinds of male sterile cytoplasm to common wheat have been discovered, the systems of hybrid wheat production based on male sterile cytoplasm are all the A-line/R-line type and all have similar problems of hybrid fertility restoration. This study confirmed that there is a new model (A-line/R*-line//R-line) for producing hybrid wheat with high fertility restoration. In the new model, the completely male sterile hybrids of A-line/R*-line can act as common A-line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00875.x
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation and structure analysis of titanium oxide nanotubes (2001)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 79 (2001), S. 3702-3704 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Well crystallized nanoscale tubular materials have been synthesized via the reaction of TiO2 crystals of either anatase or rutile phase and NaOH aqueous solution. The atomic structure of the synthesized tubular material is imaged by high-resolution transmission electron microscopy (HRTEM), and the composition of individual tubular structures is determined using selected area energy dispersive X-ray spectroscopy (EDX). Our results show that the tubular materials are well crystallized tubes with an average diameter of about 9 nm and little dispersion, and are composed of mainly titanium and oxygen. The atomic ratio of O/Ti is found, however, to vary from tube to tube. Detailed electron and x-ray diffraction studies show that the structure of our titanium oxide nanotubes do not agree with those made of TiO2 crystals with either anatase or rutile phase. HRTEM observations revealed that the titanium oxide nanotubes usually have multiple shells, in analogy with multiwalled carbon nanotubes, but the shell spacing is about 0.75 nm which is much larger than that of the carbon nanotube, and the atomic structures of different shells are well correlated. © 2001 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1423403
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  • 4
    Electronic Resource
    Electronic Resource
    Weight functions and stress intensity factors for quarter-elliptical corner cracks in fastener holes (2004)
    PO Box 1354, 9600 Garsington Road, Oxford OX4 2XG, UK. : Blackwell Science Ltd
    Fatigue & fracture of engineering materials & structures 27 (2004), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Approximate weight functions for a quarter-elliptical crack in a fastener hole were derived from a general weight function form and two reference stress intensity factors. Closed-form expressions were obtained for the coefficients of the weight functions. The derived weight functions were validated against numerical data by comparison of stress intensity factors calculated for several nonlinear stress fields. Good agreements were achieved. These derived weight functions are valid for the geometric range of 0.5 ≤a/c≤ 1.5 and 0 ≤a/t≤ 0.8 and R/t= 0.5; and are given in forms suitable for computer numerical integration. The weight functions appear to be particularly suitable for fatigue crack growth prediction of corner cracks in fastener holes and fracture analysis of such cracks in complex stress fields.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1460-2695.2004.00796.x
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  • 5
    Electronic Resource
    Electronic Resource
    Microstructures of modified RR2072 single crystal superalloys and their effects on LCF response at 950 8°C (2003)
    Oxford, UK : Blackwell Science, Ltd
    Fatigue & fracture of engineering materials & structures 26 (2003), S. 0 
    Details
    ISSN: 1460-2695
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Effects of microstructural modifications, that are a consequence of adding minor grain boundary strengthening elements (C, Hf), on the fatigue response of an experimental single crystal superalloy have been studied. Investigations show that in the modified alloys, as a consequence of the casting process, the population of pores is reduced, but the average pore size increases and larger pores occur close to the fatigue sample surface. Such porosity changes in the modified alloys are an important contributor to their low cycle fatigue (LCF) properties. At the high stress range of LCF tests undertaken, the growth of cracks initiates from pores and their location in the modified alloys are responsible for decreased fatigue lives. At lower stress ranges, crack initiation consumes a considerable proportion of fatigue life. Since crack initiation involves strain localisation at or near the specimen surface where there is interaction with the environment, the population of pores near the specimen surface and the increased length of the tests plays a significant role in governing fatigue lives. The modified alloys benefit from their lower density of pores in the vicinity of the free surface, and tend to have comparable fatigue lives to that of the base alloy at the lower stress level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1460-2695.2003.00541.x
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  • 6
    Electronic Resource
    Electronic Resource
    Air flow and particle control with different ventilation systems in a classroom (2003)
    Oxford, UK : Munksgaard International Publishers
    Indoor air 13 (2003), S. 0 
    Details
    ISSN: 1600-0668
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Architecture, Civil Engineering, Surveying , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1600-0668.2003.00186.x
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  • 7
    Electronic Resource
    Electronic Resource
    Preparation of Bioactive PDMS-Modified CaO–SiO2–TiO2 Hybrids by the Sol-Gel Method (2000)
    Springer
    Journal of sol gel science and technology 19 (2000), S. 101-105 
    Details
    ISSN: 1573-4846
    Keywords: hybrid ; bioactivity ; mechanical property ; PDMS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Much attention has been focused on the development of a new type of bioactive material with mechanical properties analogous to those of natural bone. The present authors previously showed that some polydimethylsiloxane (PDMS)-modified CaO–SiO2–TiO2 hybrids prepared by sol-gel method show apatite-forming ability in a simulated body fluid (SBF), which is indicative of bioactivity. In the present study, effects of composition of PDMS-modified CaO–SiO2–TiO2 hybrids on their bioactivity and mechanical properties were investigated. The bioactivity of the hybrids increased with decreasing PDMS content and increasing TiO2 content. Their strain at failure increased with increasing PDMS content and decreasing TiO2 content. Some samples showed high bioactivity, as well as analogous mechanical properties to those of human cancellous bones. This type of hybrids might be useful as a bone-repairing material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008774524468
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  • 8
    Electronic Resource
    Electronic Resource
    Ineffective control of murine cytomegalovirus by IE1-specific cytotoxic T lymphocytes during protracted infection in the lung (2000)
    Springer
    Archives of virology 145 (2000), S. 1291-1304 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary.  Interstitial pneumonia caused by cytomegalovirus (CMV) is a fatal disease in immunocompromised patients. In order to examine the defense mechanism against the virus in the lung, we employed an intratracheal infection model in susceptible mice. In mice infected intratracheally with murine CMV, a protracted infection was observed where infectious virus was detected up to 21 days of infection. During this prolonged infection, massive accumulation in the lung of CD8+ T cells with activated phenotypes occurred and these CD8+ T cells showed direct ex vivo cytolytic activity against target cells pulsed with the nonamer peptide derived from IE1 protein of the virus, which has been shown to be the dominant epitope recognized by most of virus-specific CTL. Moreover, adoptive transfer of in vitro induced IE1 peptide-specific CTL line showed no anti-virus effect in the lung, although they were effective in the spleen. Hence, there is reason to assume the IE1-specific CTL induced in vivo or in vitro plays limited roles during the prolonged infection in the lung.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050070091
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  • 9
    Electronic Resource
    Electronic Resource
    Determination of proteins at nanogram levels by their quenching effect on large particle scattering of colloidal silver chloride (2000)
    Springer
    Fresenius' journal of analytical chemistry 366 (2000), S. 863-868 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A novel quantitative method for the determination of proteins in aqueous solutions has been based on the quenching of the resonance scattering light of colloidal silver chloride in the presence of proteins. The detection limits for eight kinds of proteins (BSA, HSA, egg albumin, human γ-IgG,α-chymotrypsin, E. Coli. alpsase, myoglobin, α-casein) were at about 8 ng/mL; the linear ranges of the calibration curves were 10–400 ng/mL ¶under optimal conditions,except for human γ-IgG (20–¶400 ng/mL), myoglobin (10–300 ng/mL), and α-casein (10–300 ng/mL). Three wavelengths (398 nm, 475 nm, 499 nm) were all suitable for the determination and any acidity from pH 3.0 to pH 9.0 could be chosen. A few non-protein substances at high concentration levels interfered with this method, but this problem could simply be overcome by diluting the samples before the assay. Mechanism studies showed that the quenching effect of proteins on the scattering light of colloidal silver chloride was mainly due to the coagulation of AgCl particles retarded by protein. The method was employed for the determination of total protein in human serum with sactifactory results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051586
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  • 10
    Electronic Resource
    Electronic Resource
    Application of magdala red as a fluorescence probe in the determination of nucleic acids (2000)
    Springer
    Fresenius' journal of analytical chemistry 366 (2000), S. 303-306 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A fluorescence quenching method was developed for the rapid determination of DNA and RNA using magdala red as fluorescence probe. In weakly acidic ¶medium, the fluorescence of magdala red (λex〉lem = 54055 nm) can be largely quenched by DNA or RNA. The calibration graphs are linear over the range 0.01–¶1.2 μg/mL for both calf thymus DNA (CT DNA) and salmon DNA (SM DNA), and 0.015–1.0 μg/mL for yeast RNA, respectively. The corresponding detection limits are ¶6.0 ng/mL for CT DNA, 7.0 ng/mL for SM DNA and ¶15.0 ng/mL for yeast RNA, respectively. CT DNA could be determined in the presence of 20% (w/w) yeast RNA, and the relative standard deviation of six replicate measurements is 3.18% for 400 ng/mL of CT DNA. Interference from coexisting substances in the determination of DNA was also examined. Real samples were determined with satisfactory results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050057
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